{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T03:22:35Z","timestamp":1785813755130,"version":"3.56.0"},"reference-count":21,"publisher":"The Korean Society for Reproductive Medicine","issue":"1","license":[{"start":{"date-parts":[[2022,2,28]],"date-time":"2022-02-28T00:00:00Z","timestamp":1646006400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["ecerm.org"],"crossmark-restriction":true},"short-container-title":["Clin Exp Reprod Med"],"published-print":{"date-parts":[[2022,3,1]]},"abstract":"<jats:p>Objective: We investigated the effect of supplementing fertilization medium and\/or culture medium with astaxanthin (AST) on the two phases of in vitro fertilization: gamete fertilization and embryo development.  Methods: Mouse cumulus-oocyte complexes were divided into four groups with 5 \u00b5M AST added to the fertilization medium (group 3, n=300), culture medium (group 2, n=300), or both media (group 4, n=290). No AST was added to the control group (group 1, n=300). Results: The fertilization rate was significantly higher (p&lt;0.001) in the groups using AST supplemented fertilization medium (group 3 [79.0%] and group 4 [81.4%]) than those without AST (group 1 [56.3%] and group 2 [52.3%]). The blastocyst rate calculated from the two-cell stage was significantly lower (p&lt;0.001) in the groups using AST-supplemented embryo culture medium (groups 2 [58.0%] and 4 [62.3%]) than in those without AST (groups 1 [82.8%] and 3 [79.8%]). The blastocyst rate calculated from the number of inseminated oocytes was highest in group 3 (189\/300, 63.0%) and lowest in group 2 (91\/300, 30.3%) with statistical significance compared to other groups (p&lt;0.001). There were significantly higher numbers of cells in the inner cell mass and trophectoderm, as well as significantly higher total blastocyst cell counts, in group 3 than in the control group.Conclusion: An increased blastocyst formation rate and high-quality blastocysts were found only in the fertilization medium that had been supplemented with AST. In contrast, AST supplementation of the embryo culture medium was found to impair embryo development.<\/jats:p>","DOI":"10.5653\/cerm.2021.04770","type":"journal-article","created":{"date-parts":[[2022,2,28]],"date-time":"2022-02-28T08:24:23Z","timestamp":1646036663000},"page":"26-32","update-policy":"https:\/\/doi.org\/10.5653\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Effects of astaxanthin supplementation in fertilization medium and\/or culture medium on the fertilization and development of mouse oocytes"],"prefix":"10.5653","volume":"49","author":[{"given":"Chonthicha","family":"Tana","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pareeya","family":"Somsak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Waraporn","family":"Piromlertamorn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Usanee","family":"Sanmee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3624","reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1186\/s12958-018-0391-5"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2004.02.121"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dew098"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dex330"},{"key":"ref5","first-page":"621","volume-title":"Selenium and vitamin E improve the <italic>in vitro<\/italic> maturation, fertilization and culture to blastocyst of porcine oocytes","author":"Tareq","year":"2012"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1111\/jog.12933"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.14715\/cmb\/2018.64.4.16"},{"key":"ref8","first-page":"128","volume-title":"Astaxanthin: sources, extraction, stability, biological activities and its commercial application. A review","author":"Ambati","year":"2014"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1021\/jf991106k"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016\/s0015-0282(00)00518-5"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.39.4.471"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1586\/17474108.3.4.539"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1186\/1477-7827-12-112"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1186\/1477-7827-3-28"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1080\/10408690590957188"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1016\/s0005-2736(01)00326-1"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1071\/rd17271"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1016\/j.repbio.2015.01.002"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-61779-776-7_16"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2008.04.041"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.20471\/acc.2019.58.04.20"}],"container-title":["Clinical and Experimental Reproductive Medicine"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/ecerm.org\/upload\/pdf\/cerm-2021-04770.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/ecerm.org\/journal\/view.php?doi=10.5653\/cerm.2021.04770","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/ecerm.org\/upload\/pdf\/cerm-2021-04770.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,28]],"date-time":"2022-02-28T08:24:51Z","timestamp":1646036691000},"score":1,"resource":{"primary":{"URL":"http:\/\/ecerm.org\/journal\/view.php?doi=10.5653\/cerm.2021.04770"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3,1]]},"references-count":21,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2022,3]]}},"URL":"https:\/\/doi.org\/10.5653\/cerm.2021.04770","relation":{},"ISSN":["2233-8233","2233-8241"],"issn-type":[{"value":"2233-8233","type":"print"},{"value":"2233-8241","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,3,1]]},"assertion":[{"value":"2021-06-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-09-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-02-28","order":2,"name":"published","label":"Published online","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-03-01","order":3,"name":"published","label":"Print publication","group":{"name":"publication_history","label":"Publication History"}}]}}