{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T17:56:45Z","timestamp":1784915805456,"version":"3.55.0"},"reference-count":34,"publisher":"The Korean Society for Reproductive Medicine","issue":"2","license":[{"start":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T00:00:00Z","timestamp":1767571200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005851","name":"Shahid Beheshti University of Medical Sciences","doi-asserted-by":"publisher","award":["43006300"],"award-info":[{"award-number":["43006300"]}],"id":[{"id":"10.13039\/501100005851","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["ecerm.org"],"crossmark-restriction":true},"short-container-title":["Clin Exp Reprod Med"],"published-print":{"date-parts":[[2026,6,1]]},"abstract":"<jats:p>Objective: Cyclophosphamide (CP), a chemotherapeutic agent, has been shown to inhibit spermatogenesis. Accordingly, the primary objective of this study was to evaluate the potential therapeutic benefits of quercetin\u2011loaded nanoselenium (quercetin\u2011loaded selenium nanoparticles [SeNPs]) in mice treated with CP.Methods: Thirty\u2011five adult male mice were randomly assigned to five groups (n=7 per group): control, quercetin\u2011loaded SeNPs (20 mg\/kg, daily for 5 weeks), CP (200 mg\/kg, single dose), treatment A (CP+quercetin\u2011loaded SeNPs), and treatment B (CP+quercetin, 20 mg\/kg daily for 5 weeks). Sperm parameters, DNA fragmentation index, catalase activity, levels of glutathione (GSH), glutathione disulfide (GSSG), malondialdehyde (MDA), and reactive oxygen species (ROS) were evaluated in all groups, along with histological assessments of testicular tissue.Results: In CP\u2011treated mice, administration of quercetin\u2011loaded SeNPs (treatment A) significantly improved sperm parameters, including total count, motility, morphology, and DNA integrity. Treatment also markedly increased the numbers of spermatogonia, primary spermatocytes, spermatids, Sertoli cells, and Leydig cells in testicular tissue. Furthermore, treatment with quercetin\u2011loaded SeNPs resulted in a significant increase in catalase activity and GSH levels while significantly reducing GSSG, MDA, and ROS levels in CP\u2011induced testicular damage.Conclusion: These findings suggest that quercetin\u2011loaded SeNPs enhance spermatogenesis in a CP\u2011induced mouse model by improving the antioxidant profile and testicular stereological parameters.<\/jats:p>","DOI":"10.5653\/cerm.2025.07801","type":"journal-article","created":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T04:53:36Z","timestamp":1768366416000},"page":"162-172","update-policy":"https:\/\/doi.org\/10.5653\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Effects of quercetin-loaded nanoselenium on spermatogenesis in a mouse model of cyclophosphamide-induced testicular damage"],"prefix":"10.5653","volume":"53","author":[{"given":"Mahsa 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