{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T05:04:28Z","timestamp":1780031068997,"version":"3.53.1"},"reference-count":27,"publisher":"The Korean Society for Reproductive Medicine","issue":"2","license":[{"start":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T00:00:00Z","timestamp":1765843200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["ecerm.org"],"crossmark-restriction":true},"short-container-title":["Clin Exp Reprod Med"],"published-print":{"date-parts":[[2026,6,1]]},"abstract":"<jats:p>Objective: This study aimed to compare aneuploidy rates and clinical outcomes between trophectoderm biopsy at fresh blastocyst (biopsy-fresh) followed by vitrification and biopsy at vitrified-warmed blastocyst (biopsy-vitri) followed by next day transfer (without re-vitrification).Methods: This retrospective study included 844 patients undergoing 844 cycles conducted from August 2019 to December 2023. Preimplantation genetic testing for aneuploidy (PGT-A) was performed via trophectoderm biopsy using array comparative genomic hybridization or next-generation sequencing for comprehensive 24-chromosome screening. Patients were divided into two groups based on the blastocyst status at the time of biopsy: the biopsy-fresh group (531 patients) and the biopsy-vitri group (313 patients).Results: The clinical pregnancy rate was significantly higher in the biopsy-fresh group compared to the biopsy-vitri group (58.7% vs. 45.6%; odds ratio [OR], 1.695; 95% confidence interval [CI], 1.215 to 2.364; &lt;i&gt;p&lt;\/i&gt;=0.002). Furthermore, the biopsy-fresh group showed higher implantation rates (45.6% vs. 32.1%; OR, 1.767; 95% CI, 1.274 to 2.451; &lt;i&gt;p&lt;\/i&gt;=0.002), ongoing pregnancy or live birth rates per cycle (48.0% vs. 35.8%; OR, 1.652; 95% CI, 1.177 to 2.319; &lt;i&gt;p&lt;\/i&gt;=0.004), and rates of good-quality blastocysts (57.1% vs. 32.1%, &lt;i&gt;p&lt;\/i&gt;&lt;0.001) compared with the biopsy-vitri group. Miscarriage rates did not differ significantly between the groups (18.2% vs. 21.4%; OR, 0.818; 95% CI, 0.457 to 1.465; &lt;i&gt;p&lt;\/i&gt;=0.501).Conclusion: Biopsy at fresh blastocyst demonstrated superior clinical outcomes compared with biopsy at vitrified-warmed blastocyst, likely due to better embryo quality. Both biopsy at fresh blastocyst and vitrified-warmed blastocyst remain viable options for PGT-A, with biopsy at vitrified-warmed blastocyst serving as a practical alternative. Embryo quality and euploid status continue to be critical considerations for embryo transfer selection.<\/jats:p>","DOI":"10.5653\/cerm.2025.07850","type":"journal-article","created":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T08:59:48Z","timestamp":1765875588000},"page":"121-127","update-policy":"https:\/\/doi.org\/10.5653\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Aneuploidy rates and clinical outcomes in vitrified-warmed blastocyst transfer cycles: comparison of biopsy at fresh blastocyst versus vitrified-warmed blastocyst"],"prefix":"10.5653","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5285-5076","authenticated-orcid":true,"given":"Jun 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