{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T05:04:28Z","timestamp":1780031068965,"version":"3.53.1"},"reference-count":31,"publisher":"The Korean Society for Reproductive Medicine","issue":"2","license":[{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["ecerm.org"],"crossmark-restriction":true},"short-container-title":["Clin Exp Reprod Med"],"published-print":{"date-parts":[[2026,6,1]]},"abstract":"<jats:p>Objective: Hydroxyethyl piperazine ethane sulfonic acid (HEPES)-buffered media have long been employed in assisted reproductive technology (ART) procedures; however, concerns persist regarding the impact of zwitterionic buffers on intrinsic cellular mechanisms. Limited studies have compared ART outcomes using bicarbonate media versus HEPES-buffered media during intracytoplasmic sperm injection (ICSI). This study compared the efficacy of two media used in ICSI: bicarbonate medium (one-step) and HEPES-buffered medium (SynVitro Flush).Methods: This retrospective study analyzed data from 200 ICSI cycles performed in 2023. Metaphase II oocytes were allocated to a bicarbonate medium (SAGE 1-STEP; Origio) or a HEPES-buffered medium (SynVitro Flush) during the ICSI procedure. Outcomes including fertilization rate, embryo quality and development, implantation rate, clinical pregnancy rate, miscarriage rate, and live birth rate were compared between the two groups.Results: The fertilization rate was significantly higher in the one-step medium (89.04%) than in the SynVitro medium (82.30%; &lt;i&gt;p&lt;\/i&gt;&lt;0.001). The one-step medium also yielded more cleaved embryos and higher-quality blastocysts (class A\/B; &lt;i&gt;p&lt;\/i&gt;&lt;0.05). Consequently, more embryos were frozen in the one-step group (264 vs. 151), and higher embryo utilization rates were observed. Clinical pregnancy and live birth rates were significantly higher in the one-step group (&lt;i&gt;p&lt;\/i&gt;&lt;0.05). No significant differences were found in chemical pregnancy rates or live birth rates among cycles utilizing frozen embryos (&lt;i&gt;p&lt;\/i&gt;&gt;0.05). Additionally, there were no differences observed in miscarriage rates or multiple pregnancy rates.Conclusion: The one-step bicarbonate medium improved cycle efficiency during the ICSI procedure compared to the SynVitro Flush medium. This finding underscores the importance of selecting an appropriate medium.<\/jats:p>","DOI":"10.5653\/cerm.2025.07871","type":"journal-article","created":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T23:37:02Z","timestamp":1759275422000},"page":"106-114","update-policy":"https:\/\/doi.org\/10.5653\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A comparison between the assisted reproductive technology outcomes of using two media during sperm microinjection: A bicarbonate medium (one-step) vs. a HEPES-buffered medium (SynVitro Flush)"],"prefix":"10.5653","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0133-9842","authenticated-orcid":true,"given":"Akram","family":"Hosseini","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zahra","family":"Borzouei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elham","family":"Naghshineh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hatav Ghasemi","family":"Tehrani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1133-5004","authenticated-orcid":true,"given":"Mahboubeh","family":"Vatanparast","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3624","reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eclinm.2023.102343"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.2019.10.003"},{"key":"ref3","volume-title":"Identifying the factors that influence the stability of the embryo culture system and the sensivity of the Mouse Embryo Assay: the critical role of oil in the IVF lab [dissertation]","year":"2022"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.2021.01.005"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1093\/hropen\/hoab021"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016\/j.tjog.2020.08.001"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.5935\/1518-0557.20210098"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.5935\/1518-0557.20200099"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dms061"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.21873\/invivo.11261"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.2010.03.012"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1016\/s1472-6483(10)60029-6"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1007\/s10815-024-03064-2"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dead093.520"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2005.10.050"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1016\/s0093-691x(99)00020-5"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2017.10.004"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1016\/s0015-0282(00)00518-5"},{"key":"ref19","first-page":"13","volume-title":"Early embryo development in V-ONESTEP, an innovative single step culture medium used for human embryo culture: a study","author":"Uhde","year":"2021"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.2023.04.018"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod34.2.349"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1016\/0093-691x(89)90468-8"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.3109\/10715769309056520"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2008.06.092"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1996.0406"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1006\/abio.2000.4848"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.5935\/1518-0557.20180024"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1007\/s10815-019-01621-8"},{"key":"ref29","doi-asserted-by":"publisher","DOI":"10.1186\/1477-7827-8-83"},{"key":"ref30","doi-asserted-by":"publisher","DOI":"10.1007\/s10815-011-9582-0"},{"key":"ref31","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmu040"}],"container-title":["Clinical and Experimental Reproductive Medicine"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/ecerm.org\/upload\/pdf\/cerm-2025-07871.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/ecerm.org\/journal\/view.php?doi=10.5653\/cerm.2025.07871","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/ecerm.org\/upload\/pdf\/cerm-2025-07871.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T04:39:56Z","timestamp":1780029596000},"score":1,"resource":{"primary":{"URL":"http:\/\/ecerm.org\/journal\/view.php?doi=10.5653\/cerm.2025.07871"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,1]]},"references-count":31,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,6]]}},"URL":"https:\/\/doi.org\/10.5653\/cerm.2025.07871","relation":{},"ISSN":["2233-8233","2233-8241"],"issn-type":[{"value":"2233-8233","type":"print"},{"value":"2233-8241","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,1]]},"assertion":[{"value":"2025-02-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-01","order":2,"name":"published","label":"Published online","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-01","order":3,"name":"published","label":"Print publication","group":{"name":"publication_history","label":"Publication History"}}]}}