{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T15:04:01Z","timestamp":1788879841720,"version":"build-2803163510"},"reference-count":48,"publisher":"Asian Australasian Association of Animal Production Societies","issue":"1","license":[{"start":{"date-parts":[[2021,3,2]],"date-time":"2021-03-02T00:00:00Z","timestamp":1614643200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Unique Biotech Ltd","award":["CR\/CARI-1\/NFT\/2019"],"award-info":[{"award-number":["CR\/CARI-1\/NFT\/2019"]}]}],"content-domain":{"domain":["animbiosci.org"],"crossmark-restriction":true},"short-container-title":["Anim Biosci"],"published-print":{"date-parts":[[2022,1,1]]},"abstract":"<jats:p>Objective: This study was conducted to investigate the effects of dietary multi-strain probiotic (MSP) (Bacillus coagulans Unique IS2 + Bacillus subtillis UBBS14 + Saccharomyces boulardii Unique 28) on performance, gut morphology and expression of nutrient transporter related genes in broiler chickens.Methods: A total of 256 (4\u00d78\u00d78) day-old CARIBRO Vishal commercial broiler chicks of uniform body weight were randomly distributed into four treatments with 8 replicates each and having eight chicks in each replicate. Four dietary treatments were T&lt;sub&gt;1&lt;\/sub&gt; (negative control-basal diet), T&lt;sub&gt;2&lt;\/sub&gt; (positive control-antibiotic bacitracin methylene disalicylate at 20 mg\/kg diet), T&lt;sub&gt;3&lt;\/sub&gt; (MSP at 10&lt;sup&gt;7&lt;\/sup&gt; colony-forming unit [CFU]\/g feed), and T&lt;sub&gt;4&lt;\/sub&gt; (MSP at 10&lt;sup&gt;8&lt;\/sup&gt; CFU\/g feed).Results: During 3 to 6 weeks and 0 to 6 weeks, the body weight gain increased significantly (p&lt;0.05) in T&lt;sub&gt;3&lt;\/sub&gt; and T&lt;sub&gt;4&lt;\/sub&gt; groups. The feed intake significantly (p&lt;0.05) reduced from T1 to T&lt;sub&gt;3&lt;\/sub&gt; during 0 to 3 weeks and the feed conversion ratio also significantly (p&lt;0.05) improved in T&lt;sub&gt;3&lt;\/sub&gt; and T&lt;sub&gt;4&lt;\/sub&gt; during 0 to 6 weeks. The humoral and cell mediated immune response and the weight of immune organs were also significantly (p&lt;0.05) improved in T&lt;sub&gt;3&lt;\/sub&gt; and T&lt;sub&gt;4&lt;\/sub&gt;. However, significant (p&lt;0.05) dietary effects were observed on intestinal histo-morphometry of ileum in T&lt;sub&gt;3&lt;\/sub&gt; followed by T&lt;sub&gt;4&lt;\/sub&gt; and T&lt;sub&gt;2&lt;\/sub&gt;. At 14 d post hatch, the relative gene expression of glucose transporter (GLUT5), sodium-dependent glucose transporter (SGLT1) and peptide transporter (PepT1) showed a significant (p&lt;0.05) up-regulating pattern in T&lt;sub&gt;2&lt;\/sub&gt;, T&lt;sub&gt;3&lt;\/sub&gt;, and T&lt;sub&gt;4&lt;\/sub&gt;. Whereas, at 21 d post hatch, the gene expression of SGLT1 and PepT1 was significantly (p&lt;0.05) downregulated in MSP supplemented treatments T&lt;sub&gt;3&lt;\/sub&gt; and T&lt;sub&gt;4&lt;\/sub&gt;.Conclusion: The supplementation of MSP at 10&lt;sup&gt;7&lt;\/sup&gt; CFU\/g diet showed significant effects with improved performance, immune response, gut morphology and expression of nutrient transporter genes. Thus, the MSP could be a suitable alternative to antibiotic growth promoters in chicken diets.<\/jats:p>","DOI":"10.5713\/ab.20.0749","type":"journal-article","created":{"date-parts":[[2021,3,2]],"date-time":"2021-03-02T00:36:10Z","timestamp":1614645370000},"page":"64-74","update-policy":"https:\/\/doi.org\/10.5713\/crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["The effect of multi-strain probiotics as feed additives on performance, immunity, expression of nutrient transporter genes and gut morphometry in broiler chickens"],"prefix":"10.5713","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1439-2477","authenticated-orcid":true,"given":"Avishek","family":"Biswas","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7648-4043","authenticated-orcid":true,"given":"Kapil","family":"Dev","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8083-8177","authenticated-orcid":true,"given":"Pramod K","family":"Tyagi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7607-6955","authenticated-orcid":true,"given":"Asitbaran","family":"Mandal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3683","reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1080\/09712119.2019.1618311"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1093\/japr\/14.4.750"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.4314\/sajas.v47i5.9"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2006.12.013"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.3920\/BM2014.0062"},{"key":"ref6","first-page":"135","volume-title":"The effect of probiotic and prebiotic feed supplementation on chicken health and gut microflora: a review","author":"Ohimain","year":"2012"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.18016\/ksujns.40137"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1038\/srep01163"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1016\/j.csbj.2018.03.002"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1016\/j.aninu.2020.03.002"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.5897\/JCAB.9000059"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.3382\/ps.0590001"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.3382\/ps.0620205"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.3382\/ps.0690403"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.3382\/ps.0670989"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/30.9.e36"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.3382\/ps.2008-00244"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.18805\/ijar.B-3420"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.3382\/ps.2008-00224"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1080\/00071668.2011.636029"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1093\/ps\/82.4.627"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2007-3007"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2011.09.133"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1080\/17450390500216928"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1016\/j.aninu.2018.08.002"},{"key":"ref26","first-page":"1","volume-title":"Applications of probiotics in poultry: Enhancing immunity and beneficial effects on production performances and health - 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