{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T03:17:05Z","timestamp":1781493425007,"version":"3.54.1"},"reference-count":35,"publisher":"Asian Australasian Association of Animal Production Societies","issue":"12","license":[{"start":{"date-parts":[[2022,4,29]],"date-time":"2022-04-29T00:00:00Z","timestamp":1651190400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2021M691607"],"award-info":[{"award-number":["2021M691607"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Research and demonstration on the key technology of crop straw and agricultural and sideline products feeding","award":["2019C10010"],"award-info":[{"award-number":["2019C10010"]}]}],"content-domain":{"domain":["animbiosci.org"],"crossmark-restriction":true},"short-container-title":["Anim Biosci"],"published-print":{"date-parts":[[2022,12,1]]},"abstract":"<jats:p>Objective: The observation that temperate C&lt;sub&gt;3&lt;\/sub&gt; and tropical C&lt;sub&gt;4&lt;\/sub&gt; forage silages easily produce large amounts of ethanol or acetic acid has puzzled researchers for many years. Hence, this study aimed to assess the effects of epiphytic microbiota from C&lt;sub&gt;3&lt;\/sub&gt; forages (Italian ryegrass and oat) on fermentative products and bacterial community structure in C&lt;sub&gt;4&lt;\/sub&gt; forage (sorghum) silage.Methods: Through microbiota transplantation and \u03b3-ray irradiation sterilization, the irradiated sorghum was treated: i) sterile distilled water (STSG); ii) epiphytic microbiota from sorghum (SGSG); iii) epiphytic microbiota from Italian ryegrass (SGIR); iv) epiphytic microbiota from oat (SGOT).Results: After 60 days, all the treated groups had high lactic acid (&gt;63.0 g\/kg dry matter [DM]) contents and low pH values (&lt;3.70), acetic acid (&lt;14.0 g\/kg DM) and ammonia nitrogen (&lt;80.0 g\/kg total nitrogen) contents. Notably, SGIR (59.8 g\/kg DM) and SGOT (77.6 g\/kg DM) had significantly (p&lt;0.05) higher ethanol concentrations than SGSG (14.2 g\/kg DM) on day 60. After 60 days, &lt;i&gt;Lactobacillus&lt;\/i&gt; were predominant genus in three treated groups. Higher proportions of &lt;i&gt;Chishuiella&lt;\/i&gt; (12.9%) and &lt;i&gt;Chryseobacterium&lt;\/i&gt; (7.33%) were first found in silages. The ethanol contents had a positive correlation (p&lt;0.05) with the abundances of &lt;i&gt;Chishuiella&lt;\/i&gt;, &lt;i&gt;Acinetobacter&lt;\/i&gt;, &lt;i&gt;Stenotrophomonas&lt;\/i&gt;, &lt;i&gt;Chryseobacterium&lt;\/i&gt;, and &lt;i&gt;Sphingobacterium&lt;\/i&gt;.Conclusion: The epiphytic bacteria on raw materials played important roles in influencing the silage fermentation products between temperate C&lt;sub&gt;3&lt;\/sub&gt; and tropical C&lt;sub&gt;4&lt;\/sub&gt; forages. The quantity and activity of hetero-fermentative &lt;i&gt;Lactobacillus&lt;\/i&gt;, &lt;i&gt;Chishuiella&lt;\/i&gt;, &lt;i&gt;Acinetobacter&lt;\/i&gt;, &lt;i&gt;Stenotrophomonas&lt;\/i&gt;, &lt;i&gt;Chryseobacterium&lt;\/i&gt;, and &lt;i&gt;Sphingobacterium&lt;\/i&gt; may be the key factors for the higher ethanol contents and DM loss in silages.<\/jats:p>","DOI":"10.5713\/ab.21.0543","type":"journal-article","created":{"date-parts":[[2022,4,29]],"date-time":"2022-04-29T07:02:47Z","timestamp":1651215767000},"page":"1860-1870","update-policy":"https:\/\/doi.org\/10.5713\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Fermentative products and bacterial community structure of C4 forage silage in response to epiphytic microbiota from C3 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