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However, identifying the causal variant remains a huge challenge. This study aimed to detect single nucleotide polymorphisms (SNPs) and candidate genes for the number of mummified (NM) piglets using the imputed whole-genome sequence (WGS) and validate the potential candidate genes.Methods: The imputed WGS was introduced from genotyping-by-sequencing (GBS) using a multi-breed reference population. We performed genome-wide association studies (GWAS) for NM piglets at birth from a Landrace pig populatiGWAS peak located on SSC11: 0.10 to 7.11 Mbp (Top SNP, SSC11:1,889,658 bp; p = 9.98E-13) was identified in cyclin dependent kinase on. A total of 300 Landrace pigs were genotyped by GBS. The whole-genome variants were imputed, and 4,252,858 SNPs were obtained. Various molecular experiments were conducted to determine how the genes affected NM in pigs.Results: A strong GWAS peak located on SSC11: 0.10 to 7.11 Mbp (Top SNP, SSC11:1,889,658 bp; p = 9.98E-13) was identified in cyclin dependent kinase 8 (&lt;i&gt;CDK8&lt;\/i&gt;) gene, which plays a crucial role in embryonic retardation and lethality. Based on the molecular experiments, we found that Y-box binding protein 1 (&lt;i&gt;YBX1&lt;\/i&gt;) was a crucial transcription factor for &lt;i&gt;CDK8&lt;\/i&gt;, which mediated the effect of &lt;i&gt;CDK8&lt;\/i&gt; in the proliferation of porcine ovarian granulosa cells via transforming growth factor beta\/small mother against decapentaplegic signaling pathway, and, as a consequence, affected embryo quality, indicating that this pathway may be contributing to mummified fetal in pigs.Conclusion: A powerful imputation-based association study was performed to identify genes associated with NM in pigs. &lt;i&gt;CDK8&lt;\/i&gt; was suggested as a functional gene for the proliferation of porcine ovarian granulosa cells, but further studies are required to determine causative mutations and the effect of loci on NM in pigs.<\/jats:p>","DOI":"10.5713\/ab.22.0115","type":"journal-article","created":{"date-parts":[[2022,9,6]],"date-time":"2022-09-06T20:58:25Z","timestamp":1662497905000},"page":"29-42","update-policy":"https:\/\/doi.org\/10.5713\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Whole-genome sequence association study identifies cyclin dependent kinase 8 as a key gene for the number of mummified 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