{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,21]],"date-time":"2026-06-21T02:53:39Z","timestamp":1782010419561,"version":"3.54.5"},"reference-count":28,"publisher":"Asian Australasian Association of Animal Production Societies","issue":"5","license":[{"start":{"date-parts":[[2022,11,14]],"date-time":"2022-11-14T00:00:00Z","timestamp":1668384000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009088","name":"University of Veterinary Medicine Vienna","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100009088","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["animbiosci.org"],"crossmark-restriction":true},"short-container-title":["Anim Biosci"],"published-print":{"date-parts":[[2023,5,1]]},"abstract":"Objective: Dietary phytase increases bioavailability of phytate-bound phosphorus (P) in pig nutrition affecting dietary calcium (Ca) to P ratio, intestinal uptake, and systemic utilization of both minerals, which may contribute to improper bone mineralization. We used phytase to assess long-term effects of two dietary available P (aP) levels using a one-phase feeding system on gene expression related to Ca and P homeostasis along the intestinal tract and in the kidney, short-chain fatty acids in stomach, cecum, and colon, serum, and bone parameters in growing gilts and barrows.Methods: Growing pigs (37.9\u00b16.2 kg) had either free access to a diet without (Con; 75 gilts and 69 barrows) or with phytase (650 phytase units; n = 72\/diet) for 56 days. Samples of blood, duodenal, jejunal, ileal, cecal, and colonic mucosa and digesta, kidney, and metacarpal bones were collected from 24 pigs (6 gilts and 6 barrows per diet).Results: Phytase decreased daily feed intake and average daily gain, whereas aP intake increased with phytase versus Con diet (p<0.05). Gilts had higher colonic expression of <i>TRPV5<\/i>, <i>CDH1<\/i>, <i>CLDN4<\/i>, <i>ZO1<\/i>, and <i>OCLN<\/i> and renal expression of <i>TRPV5<\/i> and <i>SLC34A3<\/i> compared to barrows (p<0.05). Phytase increased duodenal expression of <i>TRPV5<\/i>, <i>TRPV6<\/i>, <i>CALB1<\/i>, <i>PMCA1b<\/i>, <i>CDH1<\/i>, <i>CLDN4<\/i>, <i>ZO1<\/i>, and <i>OCLN<\/i> compared to Con diet (p<0.05). Furthermore, phytase increased expression of <i>SCL34A2<\/i> in cecum and of <i>FGF23<\/i> and <i>CLDN4<\/i> in colon compared to Con diet (p<0.05). Alongside, phytase decreased gastric propionate, cecal valerate, and colonic caproate versus Con diet (p<0.05). Phytase reduced cortical wall thickness and index of metacarpal bones (p<0.05).Conclusion: Gene expression results suggested an intestinal adaptation to increased dietary aP amount by increasing duodenal trans- and paracellular Ca absorption to balance the systemically available Ca and P levels, whereas no adaption of relevant gene expression in kidney occurred. Greater average daily gain in barrows related to higher feed intake.<\/jats:p>","DOI":"10.5713\/ab.22.0251","type":"journal-article","created":{"date-parts":[[2022,11,13]],"date-time":"2022-11-13T20:47:42Z","timestamp":1668372462000},"page":"740-752","update-policy":"https:\/\/doi.org\/10.5713\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Available phosphorus levels modulate gene expression related to intestinal calcium and phosphorus absorption and bone parameters differently in gilts and barrows"],"prefix":"10.5713","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5079-0440","authenticated-orcid":true,"given":"Julia 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