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The trial lasted 35 d.Results: Results showed that dietary Api-PR19 addition increased (p&lt;0.05) the average daily feed intake, average daily gain and decreased (p&lt;0.05) feed conversion ratio (FCR) during 1 to 21 d compared with the CON group. The digestibility of dry matter and crude protein were higher in AGP and ABP groups (p&lt;0.05) where greater trypsin activity was detected in duodenum (p&lt;0.05). The ratio of villus height to crypt depth (V\/C) in duodenum and jejunum was increased at 35 d when broilers were given diets with ABP or AGP (p&lt;0.05). Besides, ABP treatments up-regulated (p&lt;0.05) the mRNA expression of &lt;i&gt;EAAT3, GLUT2, ZO-1&lt;\/i&gt;, and &lt;i&gt;Claudin-1&lt;\/i&gt; in duodenum of broilers at 35 d of age. The results of immunohistochemistry showed that ABP treatment significantly increased (p&lt;0.05) duodenal secretory immunoglobulin A (sIgA) content. In addition, 16S rRNA gene sequencing revealed that there were differences in the intestinal microbiota diversity and composition among three groups. Notably, the linear discriminant analysis effect size showed that &lt;i&gt;p_Firmicutes, g_Enterococcus, g_Carnobacterium, g_Kitasatospora&lt;\/i&gt;, and &lt;i&gt;g_Acidaminococcus&lt;\/i&gt; were dominant in ABP group. Redundancy analysis showed that these changes in gut microbiota in ABP group had correlation with growth performance, intestinal morphology, and content of sIgA.Conclusion: In general, these results indicated that dietary 330 mg\/kg Apidaecin Api-PR19 supplementation promoted growth performance of broilers by improving intestinal development, nutrients absorption, immune function and modulating intestinal microbiota.<\/jats:p>","DOI":"10.5713\/ab.23.0357","type":"journal-article","created":{"date-parts":[[2024,4,1]],"date-time":"2024-04-01T10:08:37Z","timestamp":1711966117000},"page":"1622-1634","update-policy":"https:\/\/doi.org\/10.5713\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Dietary apidaecin Api-PR19 addition enhances growth performance by regulating gut health and microbiota in 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