{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T18:48:39Z","timestamp":1778698119564,"version":"3.51.4"},"reference-count":28,"publisher":"Asian Australasian Association of Animal Production Societies","issue":"1","license":[{"start":{"date-parts":[[2024,10,24]],"date-time":"2024-10-24T00:00:00Z","timestamp":1729728000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["animbiosci.org"],"crossmark-restriction":true},"short-container-title":["Anim Biosci"],"published-print":{"date-parts":[[2025,1,1]]},"abstract":"<jats:p>Objective: This study evaluates the collaborative effect of exogenous enzyme blend and dietary nutrient density on the performance of broiler chicken.Methods: A total of 600 Ross 308 broiler chickens with same average initial body weight were randomly assigned to 5 treatments. Each treatment contained 8 replicates, and 15 birds per replicate. The diets included a control (CON) starter\/finisher (S\/F) diet with metabolizable energy (ME) 3,100\/3,200 in Kcal\/kg and crude protein (CP) content 22.0.0\/20.00 in % as (S\/F 3,100\/3,200 Kcal\/kg + CP [22.00\/20.00]%), S\/F with ME 3,060\/3,150 Kcal\/kg + CP (21.50\/19.50)% with and without the exogenous enzyme blend as (S\/F 3,060\/3,150 Kcal\/kg + [21.50\/19.50]% with, and without the exogenous enzyme blend), and lastly, S\/F with ME 3,010\/3,100 Kcal\/kg + CP (21.50\/19.50)% with, and without the exogenous enzyme blend as (S\/F 3,010\/3,100 Kcal\/kg + [21.50\/19.50]% with, and without the exogenous enzyme blend). The impact of the treatments was tested on growth performance, nutrient digestibility, blood metabolites, intestinal microflora, and morphology of broiler chicken.Results: The inclusion of exogenous enzyme blend in the nutrient-deficient diet S\/F 3,060\/3,150 + 21.50\/19.50 increased (p&lt;0.05) broilers body weight, feed conversion ratio, nutrient digestibility of CP, gross energy, phosphorus, and blood phosphorus, with tendency (p&lt;0.10) of higher dry matter. The treatment also showed lower (p&lt;0.05) total anaerobic bacteria, coliform, and higher (p&lt;0.05) villus height (VH) in the jejunum, with tendencies (p&lt;0.10) of higher lactobacillus in the ileum and caecum, and higher tendency (p&lt;0.10) of VH in duodenum and ileum.Conclusion: We concluded that the improved performance could be attributed to the potency of S\/F 3,060\/3,150 + 21.50\/19.50 supplemented with 0.05% of the multienzyme to reduce the level of potential pathogenic bacteria with an increased level of positive bacteria, which in turn creates an enabling intestinal villi structure in broiler chicken.<\/jats:p>","DOI":"10.5713\/ab.24.0233","type":"journal-article","created":{"date-parts":[[2024,10,24]],"date-time":"2024-10-24T07:13:55Z","timestamp":1729754035000},"page":"131-141","update-policy":"https:\/\/doi.org\/10.5713\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Nutrient density and the collaborative impact of exogenous enzyme blend on 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