{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T09:16:39Z","timestamp":1758273399808,"version":"3.44.0"},"reference-count":40,"publisher":"Asian Australasian Association of Animal Production Societies","issue":"10","license":[{"start":{"date-parts":[[2025,4,28]],"date-time":"2025-04-28T00:00:00Z","timestamp":1745798400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100018555","name":"Science and Technology Program of Guizhou Province","doi-asserted-by":"crossref","award":["QKHZC[2020]1Y031"],"award-info":[{"award-number":["QKHZC[2020]1Y031"]}],"id":[{"id":"10.13039\/501100018555","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Guizhou Erbiaoge Ecological Agriculture Co., Ltd."},{"name":"Guizhou University Industry-University-Research Technical Cooperation Agreement","award":["701338201207"],"award-info":[{"award-number":["701338201207"]}]},{"name":"Guizhou Liuzhi Running Mountain Pig Technology Backyard","award":["701338232107"],"award-info":[{"award-number":["701338232107"]}]}],"content-domain":{"domain":["animbiosci.org"],"crossmark-restriction":true},"short-container-title":["Anim Biosci"],"published-print":{"date-parts":[[2025,10,1]]},"abstract":"<jats:p>Objective: This study was conducted to identify lncRNAs associated with fat deposition in Kele pigs and investigate their molecular regulatory mechanisms.Methods: Six healthy 10-month-old male Kele pigs were selected as the experimental subjects and divided into high backfat thickness (&gt;5%, H) and low backfat thickness group (&lt;5%, L). Subcutaneous adipose tissue of each group was collected for whole-transcriptome RNA sequencing (RNA-seq). Subsequently, the biological functions of identified lncRNAs were investigated to explore their regulatory mechanisms on fat deposition in Kele pigs.Results: RNA-seq revealed 493 differentially expressed lncRNAs (p&lt;0.05), with &lt;i&gt;TCONS_00185937&lt;\/i&gt; and &lt;i&gt;TCONS_00161198&lt;\/i&gt; implicated in fat deposition. Correlation analysis showed that the expression of &lt;i&gt;TCONS_00185937&lt;\/i&gt; was significantly negatively correlated with backfat thickness, and the expression of &lt;i&gt;TCONS_00161198&lt;\/i&gt; was significantly positively correlated with backfat thickness, and negatively correlated with eye muscle area. The pEGFP-&lt;i&gt;TCONS_00185937&lt;\/i&gt; was transfected into subcutaneous fat precursor cells, it was found that the expression levels of &lt;i&gt;TCONS_00185937&lt;\/i&gt; and its target genes &lt;i&gt;MOGAT2&lt;\/i&gt; and &lt;i&gt;ATGL&lt;\/i&gt; were significantly higher than control group (p&lt;0.05). Similarly, pEGFP-&lt;i&gt;TCONS_00161198&lt;\/i&gt; transfected cells found that the expression levels of &lt;i&gt;TCONS_00161198&lt;\/i&gt; and its target genes &lt;i&gt;CSF3R&lt;\/i&gt; and &lt;i&gt;ACC&lt;\/i&gt; were significantly higher than control group (p&lt;0.05). The induced differentiation of transfected cells found that the OD510 absorption value of pEGFP-&lt;i&gt;TCONS_00161198&lt;\/i&gt; group was higher than that of the control group. Triglyceride content was significantly higher than that of control group (p&lt;0.01), while OD510 absorption value and triglyceride content of pEGFP-TCONS_00185937 group were lower than that of control group.Conclusion: The above results indicate that &lt;i&gt;TCONS_00161198&lt;\/i&gt; and &lt;i&gt;TCONS_00185937&lt;\/i&gt; may have regulatory effects on their target genes, thereby affecting fat deposition in pig subcutaneous fat precursor cells. This work provides a theoretical basis for the regulation of fat deposition in Kele pigs.<\/jats:p>","DOI":"10.5713\/ab.24.0900","type":"journal-article","created":{"date-parts":[[2025,4,28]],"date-time":"2025-04-28T05:21:17Z","timestamp":1745817677000},"page":"2079-2093","update-policy":"https:\/\/doi.org\/10.5713\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Discovery and functional study of lncRNAs associated with fat deposition in Kele pigs based on whole-transcriptome RNA 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