{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T07:38:35Z","timestamp":1790149115504,"version":"4.0.1"},"reference-count":36,"publisher":"Asian Australasian Association of Animal Production Societies","issue":"5","license":[{"start":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T00:00:00Z","timestamp":1766016000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Shandong Provincial Natural Science Foundation of China","award":["ZR2024MC099"],"award-info":[{"award-number":["ZR2024MC099"]}]}],"content-domain":{"domain":["animbiosci.org"],"crossmark-restriction":true},"short-container-title":["Anim Biosci"],"published-print":{"date-parts":[[2026,5,1]]},"abstract":"<jats:p>Objective: Weaning stress impairs calf health by causing growth retardation and immune dysfunction, underscoring the need for targeted interventions. Maternal bovine appeasing substance (MBAS) has been proposed as a novel therapy for stress in cattle, but its efficacy against weaning stress remains unclear. This study assessed the health impacts of weaning and evaluated MBAS as a potential intervention.Methods: Health impacts were first evaluated in 60 weaned Holstein calves. Then, another 60 healthy calves of similar age were randomly divided into two groups (n = 30\/group). Control calves received a single 2.5 mL saline spray one day pre-weaning, while the MBAS group received a single 2.5 mL spray of MBAS (100 mg\/mL).Results: Weaning stress increased diarrhea (56.52% to 78.26%) and respiratory disease incidence (10.17% to 15.88%), elevated exercise steps (32,196\u00b11,267 vs 38,226\u00b11,132, p = 0.0043), and reduced dry matter intake (2.78\u00b10.03 vs 2.59\u00b10.06 kg\/d at D4, p = 0.0041) and average daily gain (ADG, 0.89\u00b10.05 vs 0.71\u00b10.04 kg\/d, p = 0.0230) within 15 days post-weaning. MBAS improved ADG (0.92\u00b10.15 vs 1.25\u00b10.14 kg\/d at D7, p = 0.0120), lowered respiratory disease (23.07% to 17.42%) and diarrhea incidence (83.70% to 52.70%), and shortened diarrhea duration (4.32\u00b10.47 d vs 3.15\u00b10.32 d, p = 0.0090). It also reduced serum interleukin-1 (49.39\u00b12.40 vs 43.51\u00b11.64 pg\/mL, p = 0.0350), haptoglobin (58.29\u00b13.3 vs 49.40\u00b13.65 \u03bcg\/mL, p = 0.0050), and cortisol (37.42\u00b11.67 vs 33.05\u00b12.24 nmol\/L, p = 0.0310), while increasing immunoglobulin G (10.30\u00b11.46 vs 13.50\u00b11.58 \u03bcg\/mL, p = 0.0131) and interferon-\u03b3 (6.89\u00b10.47 vs 7.94\u00b10.63 ng\/L, p = 0.0330) on D5.Conclusion: MBAS alleviates weaning stress by inhibiting inflammation, enhancing immunity, and reducing anxiety, providing an effective strategy to improve calf health.<\/jats:p>","DOI":"10.5713\/ab.250567","type":"journal-article","created":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T08:14:56Z","timestamp":1766132096000},"page":"250567","update-policy":"https:\/\/doi.org\/10.5713\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Alleviating calf weaning stress: the important role of maternal bovine appeasing substance in calf husbandry management"],"prefix":"10.5713","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-0721-1138","authenticated-orcid":true,"given":"Tian-Lin","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-7110-7600","authenticated-orcid":true,"given":"Jie","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-5676-2690","authenticated-orcid":true,"given":"Jie","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-7910-1378","authenticated-orcid":true,"given":"Bo-Shi","family":"Yan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6531-1748","authenticated-orcid":true,"given":"Lin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3683","reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2018-15830"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2015-10198"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.15232\/pas.2016-01573"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2024-24521"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1017\/S1751731120001615"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2014-9062"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2013-6772"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0166926"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1093\/jas\/skae221"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.3390\/ani10091468"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0210203"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1017\/S1751731119002490"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.3390\/ani12182432"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.3390\/ani10111961"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1093\/jas\/skaa269"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1093\/jas\/skae151"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0230708"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.5187\/jast.2021.e63"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2023-23630"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1017\/S1466252314000164"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2018-15474"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.3390\/ani12213013"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2023.1249628"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2023.1181545"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1016\/j.applanim.2011.04.020"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1016\/j.applanim.2010.01.007"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1093\/jas\/skab322"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1093\/jas\/skaa339"},{"key":"ref29","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2019-18088"},{"key":"ref30","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2023-23850"},{"key":"ref31","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2020-18539"},{"key":"ref32","doi-asserted-by":"publisher","DOI":"10.3390\/molecules28030980"},{"key":"ref33","doi-asserted-by":"publisher","DOI":"10.1186\/s40104-017-0197-x"},{"key":"ref34","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2020-18166"},{"key":"ref35","doi-asserted-by":"publisher","DOI":"10.2527\/1997.7551244x"},{"key":"ref36","doi-asserted-by":"publisher","DOI":"10.3390\/ani11061545"}],"container-title":["Animal 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