{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T01:53:56Z","timestamp":1790906036193,"version":"4.1.0"},"reference-count":43,"publisher":"Asian Australasian Association of Animal Production Societies","issue":"10","license":[{"start":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T00:00:00Z","timestamp":1776297600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"National Key Research and Development Project of China","award":["2023YFD1300301"],"award-info":[{"award-number":["2023YFD1300301"]}]},{"name":"Open Project of State Key Laboratory of Animal Biotech Breeding","award":["2024SKLAB6-103"],"award-info":[{"award-number":["2024SKLAB6-103"]}]},{"name":"Promotion Plan of Young Teachers\u2019 Scientific Research and Innovation Ability in Beijing University of Agriculture","award":["QJKC-2023"],"award-info":[{"award-number":["QJKC-2023"]}],"id":[{"id":"https:\/\/ror.org\/03t9adt98","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Beijing Rural Landscape Planning and Design Engineering Technology Research Center","award":["NY2502250125"],"award-info":[{"award-number":["NY2502250125"]}]}],"content-domain":{"domain":["animbiosci.org"],"crossmark-restriction":true},"short-container-title":["Anim Biosci"],"published-print":{"date-parts":[[2026,10,1]]},"abstract":"<jats:p>Objective: Cloacal inflammation in laying ducks, often accompanied by prolapse, is a major threat to animal welfare, productivity, and intestinal health. However, the underlying molecular and metabolic mechanisms remain largely unclear.Methods: Cloacal tissues and contents from inflamed and healthy laying Pekin ducks were examined histologically, transcriptomically, and metabolomically. Cross-omics integration was used to identify coordinated molecular and metabolic changes.Results: Hematoxylin and eosin staining showed a loose and disorganized structure of the cloacal sphincter in inflamed ducks. Transcriptomic analysis identified differentially expressed genes that are enriched in immune signaling, inflammatory response, and epithelial repair pathways. Metabolomic profiling revealed significant alterations in taurine- and lipid-related metabolic pathways. Integrated analysis highlighted coordinated changes between immune-related genes (COL6A3, SPARC, PPEF1, HSD17B2, and KCNG2) and key metabolites including Hydroxytyrosol-Ac, Phytol, GG-alcohol, MeCys-SO, Thiazoline, and PEA-glu.Conclusion: The findings provide a multi-omics perspective on cloacal inflammation in laying Pekin ducks, highlighting host\u2013metabolite interactions that may play a role in immune activation and epithelial barrier dysfunction.<\/jats:p>","DOI":"10.5713\/ab.260208","type":"journal-article","created":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T01:22:31Z","timestamp":1776302551000},"page":"260208","update-policy":"https:\/\/doi.org\/10.5713\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated transcriptomic and metabolomic analyses reveal host\u2013metabolite interactions underlying cloacal inflammation in laying Pekin ducks"],"prefix":"10.5713","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9879-6147","authenticated-orcid":true,"given":"Yaxi","family":"Xu","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Beijing University of Agriculture, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5639-1755","authenticated-orcid":true,"given":"Wentao","family":"Cai","sequence":"additional","affiliation":[{"name":"Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-4515-3965","authenticated-orcid":true,"given":"Qinglei","family":"Yang","sequence":"additional","affiliation":[{"name":"Shandong Zhongxin Food Group Co., Ltd., Binzhou, 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