{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T01:38:29Z","timestamp":1792633109107,"version":"4.5.0"},"reference-count":0,"publisher":"Asian Australasian Association of Animal Production Societies","issue":"11","license":[{"start":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T00:00:00Z","timestamp":1785110400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["animbiosci.org"],"crossmark-restriction":true},"short-container-title":["Anim Biosci"],"published-print":{"date-parts":[[2026,11,1]]},"abstract":"<jats:p>Objective: The current study aimed to investigate whether miR-27b-3p regulates oxidative stress and inflammatory responses through the Nrf2\/HO-1 signaling pathway during the progression of fatty liver. Liver tissues from dairy cows suffering from fatty liver and highfat diet (HFD)-induced fatty liver mice were gathered to assess lipid accumulation, oxidative stress, inflammation, and the expression of miR-27b-3p and components of the Nrf2\/HO-1 pathway.Methods: Liver tissues from dairy cows with fatty liver and HFD-induced fatty liver mice were collected to evaluate lipid accumulation, oxidative stress, inflammation, and the expression of miR-27b-3p and components of the Nrf2\/HO-1 pathway. An in vitro steatosis model was established in AML-12 hepatocytes using palmitic acid (PA). Oxidative stress markers (reactive oxygen species [ROS], superoxide dismutase [SOD], and malondialdehyde [MDA]), inflammatory cytokines (IL-1\u03b2, IL-6, and TNF-\u03b1), and proteins associated with apoptosis were assessed. Bioinformatic analysis and dual-luciferase reporter assays were used to determine the interaction between miR-27b-3p and Nrf2. Functional assays were performed using miR-27b-3p mimetics and antagonists to evaluate its regulatory effects on the Nrf2\/HO-1 signaling pathway and downstream cellular responses.Results: Fatty liver tissues from dairy cows and HFD-fed mice exhibited significant lipid deposition, enhanced oxidative stress, and elevated inflammatory responses, accompanied by upregulation of miR-27b-3p expression and reduced Nrf2\/HO-1 signaling activity. Comparable alterations were observed in PA-treated AML-12 cells. Bioinformatic analysis and dual-luciferase experiments validated that miR-27b-3p directly binds to the 3\u00b4 untranslated region of Nrf2. Inhibition of miR-27b-3p restored Nrf2 and HO-1 expression, reduced ROS and MDA levels, enhanced SOD activity, suppressed NF-\u03baB activation, decreased pro-inflammatory cytokine production, and alleviated apoptosis. Conversely, the overexpression of miR-27b-3p further inhibited Nrf2\/HO-1 signaling and exacerbated oxidative stress, inflammation, and cellular damage.Conclusion: miR-27b-3p accelerates the progression of fatty liver toward steatohepatitis by directly inhibiting Nrf2 and downregulating the Nrf2\/HO-1 antioxidant pathway, which increase oxidative stress, inflammatory responses, and hepatocyte apoptosis. These findings identify miR-27b-3p as a key upstream regulator of redox imbalance in fatty liver and suggest that targeting miR-27b-3p could be a promising strategy for the prevention and treatment of bovine fatty liver disease.<\/jats:p>","DOI":"10.5713\/ab.260476","type":"journal-article","created":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T05:10:24Z","timestamp":1785129024000},"page":"260476","update-policy":"https:\/\/doi.org\/10.5713\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["miR-27b-3p aggravates steatohepatitis progression by targeting the Nrf2\/HO-1 pathway"],"prefix":"10.5713","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-4844-7258","authenticated-orcid":true,"given":"Kui","family":"Wang","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Yunnan Agricultural University, Kunming, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-0081-0921","authenticated-orcid":true,"given":"Hao","family":"Pan","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Yunnan Agricultural University, Kunming, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-5205-1149","authenticated-orcid":true,"given":"Yan","family":"Tian","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Yunnan Agricultural University, Kunming, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9248-0723","authenticated-orcid":true,"given":"Kangfeng","family":"Jiang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Yunnan Agricultural University, Kunming, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9105-6365","authenticated-orcid":true,"given":"Xiaobing","family":"Li","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Yunnan Agricultural University, Kunming, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3683","container-title":["Animal Bioscience"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/animbiosci.org\/upload\/pdf\/ab-260476.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/animbiosci.org\/journal\/view.php?doi=10.5713\/ab.260476","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/animbiosci.org\/upload\/pdf\/ab-260476.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T00:47:18Z","timestamp":1792630038000},"score":1,"resource":{"primary":{"URL":"http:\/\/animbiosci.org\/journal\/view.php?doi=10.5713\/ab.260476"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,11,1]]},"references-count":0,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2026,11]]}},"URL":"https:\/\/doi.org\/10.5713\/ab.260476","relation":{},"ISSN":["2765-0189","2765-0235"],"issn-type":[{"value":"2765-0189","type":"print"},{"value":"2765-0235","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,11,1]]},"assertion":[{"value":"2026-06-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-27","order":2,"name":"published","label":"Published online","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-11-01","order":3,"name":"published","label":"Print publication","group":{"name":"publication_history","label":"Publication History"}}]}}