{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T01:38:43Z","timestamp":1792633123162,"version":"4.5.0"},"reference-count":0,"publisher":"Asian Australasian Association of Animal Production Societies","issue":"11","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["animbiosci.org"],"crossmark-restriction":true},"short-container-title":["Anim Biosci"],"published-print":{"date-parts":[[2026,11,1]]},"abstract":"Objective: The conventional <i>in vitro<\/i> nutrient digestibility procedure was developed for growing pigs whereas the procedure for weanling pigs is not available. The present study aimed to develop an <i>in vitro<\/i> procedure for assessing standardized ileal digestibility (SID) of crude protein (CP) in feed ingredients for weanling pigs by modifying the conventional approach used for growing pigs.Methods: In the conventional <i>in vitro<\/i> ileal disappearance (IVID) procedure, sample solutions added with 1 mL of pepsin (10.0 g\/L) are incubated for 6.0 h in step 1 to simulate digestion in the stomach followed by adding 1 mL of pancreatin (50.0 g\/L) and incubating for 18.0 h in step 2 to simulate digestion in the small intestine. To develop an IVID procedure for weanling pigs, 3 incubation periods (6.0, 4.5, and 3.0 h in step 1 and 18.0, 13.5, and 9.0 h in step 2) and 3 enzyme concentrations (pepsin 10.0, 7.5, and 5.0 g\/L in step 1 and pancreatin 50.0, 37.5, and 25.0 g\/L in step 2) were tested in a 3\u00d73 factorial arrangement. Test ingredients were rice, corn, wheat, rapeseed meal, soybean meal, fermented soybean meal, and soy protein concentrate.Results: The IVID of CP, measured using the conventional procedure, exhibited an average of 5.0% deviation from the <i>in vivo<\/i> SID of CP in weanling pigs. Based on the quadratic response surface analysis, the use of 1 mL of pepsin at 7.6 g\/L with the incubation period of 4.4 h in step 1 followed by adding 1 mL of pancreatin at 38.1 g\/L with the incubation period of 13.3 h in step 2 minimized the absolute difference between IVID of CP and <i>in vivo<\/i> SID of CP to 3.6% on average for weanling pigs.Conclusion: The <i>in vitro<\/i> procedure for weanling pigs was refined by reducing the incubation periods and enzyme concentrations to more accurately assess the ileal digestibility of protein in feed ingredients compared with the conventional <i>in vitro<\/i> procedure.<\/jats:p>","DOI":"10.5713\/ab.260517","type":"journal-article","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T09:17:46Z","timestamp":1788254266000},"page":"260517","update-policy":"https:\/\/doi.org\/10.5713\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["An in vitro ileal disappearance procedure for estimating ileal digestibility of protein in weanling pigs"],"prefix":"10.5713","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-3624-8846","authenticated-orcid":true,"given":"Yujeong","family":"Hwang","sequence":"first","affiliation":[{"name":"Department of Animal Science, Konkuk University, Seoul, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6471-799X","authenticated-orcid":true,"given":"Yoon Soo","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Konkuk University, Seoul, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2097-717X","authenticated-orcid":true,"given":"Beob Gyun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Konkuk University, Seoul, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3683","container-title":["Animal Bioscience"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/animbiosci.org\/upload\/pdf\/ab-260517.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/animbiosci.org\/journal\/view.php?doi=10.5713\/ab.260517","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/animbiosci.org\/upload\/pdf\/ab-260517.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T00:47:19Z","timestamp":1792630039000},"score":1,"resource":{"primary":{"URL":"http:\/\/animbiosci.org\/journal\/view.php?doi=10.5713\/ab.260517"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,11,1]]},"references-count":0,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2026,11]]}},"URL":"https:\/\/doi.org\/10.5713\/ab.260517","relation":{},"ISSN":["2765-0189","2765-0235"],"issn-type":[{"value":"2765-0189","type":"print"},{"value":"2765-0235","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,11,1]]},"assertion":[{"value":"2026-06-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-07","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-01","order":2,"name":"published","label":"Published online","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-11-01","order":3,"name":"published","label":"Print publication","group":{"name":"publication_history","label":"Publication History"}}]}}