{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T08:07:43Z","timestamp":1777968463259,"version":"3.51.4"},"reference-count":41,"publisher":"Asian Australasian Association of Animal Production Societies","issue":"6","license":[{"start":{"date-parts":[[2020,4,12]],"date-time":"2020-04-12T00:00:00Z","timestamp":1586649600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"AB Vista"}],"content-domain":{"domain":["animbiosci.org"],"crossmark-restriction":true},"short-container-title":["Anim Biosci"],"published-print":{"date-parts":[[2021,6,1]]},"abstract":"<jats:p>Objective: This study assessed the effect of different levels of xylanase, \u03b2-glucanase and phytase on intestinal enzyme activities and tibia bone development in broiler chickens fed wheat-based diets.Methods: Twelve experimental diets were formulated using a 3\u00d72\u00d72 factorial design (three doses of phytase and two doses of both xylanase and \u03b2-glucanase) and offered to 648 dayold Ross 308 male chicks having 6 replicates groups with 9 birds per replicate and lasted for 35 days.Results: An interaction between the enzymes products improved (p&lt;0.01) the activity of chymotrypsin. Protein content at d 10 was highest (p&lt;0.001) with addition of phytase while general proteolytic activity (GPA) (p&lt;0.02) and lipase activity (p&lt;0.001) were decreased. At d 24, there were improvements in protein content (p&lt;0.01) and lipase (p&lt;0.04) with supplementation of superdose phytase. Addition of superdose phytase decreased in chymotrypsin (p&lt;0.02), trypsin (p&lt;0.01) and GPA (p&lt;0.001). The optimum dose of xylanase decreased the chymotrypsin activity (p = 0.05), while the GPA (p&lt;0.001) was increased with the optimum level of \u03b2-glucanase. Superdose phytase supplementation at d 10 improved maltase (p = 0.05), sucrase (p&lt;0.001) and alkaline phosphatase (p&lt;0.001) activities in the jejunum while aminopeptidase activity was highest (p&lt;0.005) with the low level of phytase. Protein content of jejunum mucosa was bigger (p&lt;0.001) in birds fed superdose phytase while maltase activity (p&lt;0.001) at d 24 was reduced by this treatment. Sucrase (p&lt;0.04) and aminopeptidase activities (p&lt;0.001) improved when diets supplemented with low levels of phytase. Tibia bone breaking strength was highest (p&lt;0.04) with addition of low level of superdose phytase or optimum level of \u03b2-glucanase. Bone dry matter content decreased (p&lt;0.04) when diets supplemented with phytase.Conclusion: From the results obtained in this study, supplementation of superdose phytase was the most effective, however, the cost-benefit analysis of the use of such a dose needs to be evaluated.<\/jats:p>","DOI":"10.5713\/ajas.19.0885","type":"journal-article","created":{"date-parts":[[2020,4,12]],"date-time":"2020-04-12T14:23:31Z","timestamp":1586701411000},"page":"1049-1060","update-policy":"https:\/\/doi.org\/10.5713\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Endogenous enzyme activities and tibia bone development of broiler chickens fed wheat-based diets supplemented with xylanase, \u03b2-glucanase and 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