{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T01:35:41Z","timestamp":1780364141677,"version":"3.54.1"},"reference-count":36,"publisher":"Asian Australasian Association of Animal Production Societies","issue":"4","license":[{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003669","name":"Korea Centers for Disease Control and Prevention","doi-asserted-by":"crossref","award":["2020ER540500"],"award-info":[{"award-number":["2020ER540500"]}],"id":[{"id":"10.13039\/501100003669","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["animbiosci.org"],"crossmark-restriction":true},"short-container-title":["Anim Biosci"],"published-print":{"date-parts":[[2021,4,1]]},"abstract":"<jats:p>Objective: The present study aimed to investigate the occurrence and species of coagulasepositive staphylococci (CoPS) and coagulase-negative staphylococci (CoNS) in retail pork meat samples collected during nationwide monitoring. The staphylococcal isolates were characterized for antimicrobial and zinc chloride resistance and enterotoxigenic potential.Methods: A total of 260 pre-packaged pork meat samples were collected from 35 retail markets in 8 provinces in Korea for isolation of staphylococci. Antimicrobial and zinc chloride resistance phenotypes, and genes associated with the resistance phenotypes were determined on the isolates. Furthermore, the presence and distribution of 19 staphylococcal enterotoxin (SE) genes and enterotoxin-like genes among the pork-associated staphylococci were determined by multiplex polymerase chain reaction-based assays using the specific primer sets.Results: A total of 29 staphylococcal strains (29\/260, 11.1%) were isolated from samples of retail pork meat, 24 (83%) of which were CoNS. The four CoNS species identified were &lt;i&gt;S. saprophyticus&lt;\/i&gt; (n = 16, 55%), &lt;i&gt;S. sciuri&lt;\/i&gt; (n = 3, 10%), &lt;i&gt;S. warneri&lt;\/i&gt; (n = 3, 10%), and &lt;i&gt;S. epidermidis&lt;\/i&gt; (n = 2, 7%). Among the 29 isolates, four methicillin-resistant CoNS (MR-CoNS; three &lt;i&gt;S. sciuri&lt;\/i&gt; and one &lt;i&gt;S. epidermidis&lt;\/i&gt;) and one methicillin-resistant CoPS (MR-CoPS; one &lt;i&gt;S. aureus&lt;\/i&gt;) were identified. In addition, a relatively high level of tetracycline (TET) resistance (52%) was confirmed in CoNS, along with a predominant distribution of &lt;i&gt;tet&lt;\/i&gt;(K). The most prevalent SEs were &lt;i&gt;sep&lt;\/i&gt; (45%), and &lt;i&gt;sen&lt;\/i&gt; (28%), which were carried by 81% of S. saprophyticus.Conclusion: These findings suggest that CoNS, especially &lt;i&gt;S. saprophyticus&lt;\/i&gt; strains, in raw pork meat could be a potential risk factor for staphylococcal food poisoning (SFP), and therefore, requires further investigation to elucidate the role of SEls in SFP and virulence of the pathogen. Our results also suggest that CoNS from raw pork meat may act as a source for transmission of antimicrobial resistance genes such as staphylococcal cassette chromosome &lt;i&gt;mec&lt;\/i&gt; and &lt;i&gt;tet&lt;\/i&gt;(K).<\/jats:p>","DOI":"10.5713\/ajas.20.0660","type":"journal-article","created":{"date-parts":[[2021,3,11]],"date-time":"2021-03-11T23:20:14Z","timestamp":1615504814000},"page":"734-742","update-policy":"https:\/\/doi.org\/10.5713\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Profiles of coagulase-positive and -negative staphylococci in retail pork: prevalence, antimicrobial resistance, enterotoxigenicity, and virulence factors"],"prefix":"10.5713","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5308-0065","authenticated-orcid":true,"given":"Gi Yong","family":"Lee","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3253-8190","authenticated-orcid":true,"given":"Soo-Jin","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3683","reference":[{"key":"ref1","first-page":"2681","volume-title":"Antimicrobial resistance and virulence gene profiles of methicillin-resistant and -susceptible <italic>Staphylococcus aureus<\/italic> from food products in Denmark","author":"Li","year":"2019"},{"key":"ref2","first-page":"436","volume-title":"Basis of virulence in enterotoxin-mediated staphylococcal food poisoning","author":"Fisher","year":"2018"},{"key":"ref3","first-page":"183","volume-title":"Detection of antibiotic resistance and classical enterotoxin genes in coagulase-negative staphylococci isolated from poultry in Poland","author":"Pyzik","year":"2019"},{"key":"ref4","first-page":"113","volume-title":"Staphylococci isolated from ready-to-eat meat - identification, antibiotic resistance and toxin gene profile","author":"Fijalkowski","year":"2016"},{"key":"ref5","first-page":"123","volume-title":"Species and staphylococcal cassette chromosome <italic>mec<\/italic> (SCC<italic>mec<\/italic>) diversity among methicillin-resistant non-<italic>Staphylococcus aureus<\/italic> staphylococci isolated from pigs","author":"Vanderhaeghen","year":"2012"},{"key":"ref6","first-page":"44","volume-title":"Multidrug resistance genes in staphylococci from animals that confer resistance to critically and highly important antimicrobial agents in human medicine","author":"Wendlandt","year":"2015"},{"key":"ref7","first-page":"e2","volume-title":"Livestock-associated methicillin-resistant <italic>Staphylococcus aureus<\/italic> in Korea: antimicrobial resistance and molecular characteristics of LA-MRSA strains isolated from pigs, pig farmers, and farm environment","author":"Back","year":"2020"},{"key":"ref8","first-page":"e00305","volume-title":"<italic>Staphylococcus aureus<\/italic> CC398: host adaptation and emergence of methicillin resistance in livestock","author":"Price","year":"2012"},{"key":"ref9","first-page":"e69","volume-title":"Prevalence and characteristics of livestock-associated methicillin-susceptible <italic>Staphylococcus aureus<\/italic> in the pork production chain in Korea","author":"Eom","year":"2019"},{"key":"ref10","first-page":"e00756","volume-title":"Zinc resistance within swine-associated methicillin-resistant <italic>Staphylococcus aureus<\/italic> isolates in the United States is associated with multilocus sequence type lineage","author":"Hau","year":"2017"},{"key":"ref11","first-page":"264","volume-title":"Combination of multiplex PCRs for staphylococcal cassette chromosome <italic>mec<\/italic> type assignment: rapid identification system for <italic>mec<\/italic>, <italic>ccr<\/italic>, and major differences in junkyard regions","author":"Kondo","year":"2007"},{"key":"ref12","first-page":"209","volume-title":"Multiplex PCR for the detection of tetracycline resistant genes","author":"Ng","year":"2001"},{"key":"ref13","first-page":"535","volume-title":"Production of gamma-hemolysin and lack of production of alpha-hemolysin by <italic>Staphylococcus aureus<\/italic> strains associated with toxic shock syndrome","author":"Clyne","year":"1988"},{"key":"ref14","first-page":"535","volume-title":"Characterization of the biodiversity of the spoilage microbiota in chicken meat using next generation sequencing and culture dependent approach","author":"Lee","year":"2017"},{"key":"ref15","first-page":"149","volume-title":"Detection of classical and newly described staphylococcal superantigen genes in coagulase-negative staphylococci isolated from bovine intramammary infections","author":"Park","year":"2011"},{"key":"ref16","first-page":"455","volume-title":"Decreased susceptibility to zinc chloride is associated with methicillin resistant <italic>Staphylococcus aureus<\/italic> CC398 in Danish swine","author":"Aarestrup","year":"2010"},{"key":"ref17","first-page":"344","volume-title":"Zinc resistance of <italic>Staphylococcus aureus<\/italic> of animal origin is strongly associated with methicillin resistance","author":"Cavaco","year":"2011"},{"key":"ref18","first-page":"125","volume-title":"High diversity of coagulase negative staphylococci species in wild boars, with low antimicrobial resistance rates but detection of relevant resistance genes","author":"Mama","year":"2019"},{"key":"ref19","first-page":"949","volume-title":"Prevalence of methicillin-resistant <italic>Staphylococcus aureus<\/italic> and other <italic>Staphylococcus<\/italic> species in raw meat samples intended for human consumption in Benin city, Nigeria: implications for public health","author":"Igbinosa","year":"2016"},{"key":"ref20","first-page":"e30092","volume-title":"MRSA in conventional and alternative retail pork products","author":"O\u2019Brien","year":"2012"},{"key":"ref21","first-page":"2767","volume-title":"<italic>Staphylococcus aureus<\/italic> isolated from retail meat and meat products in China: incidence, antibiotic resistance and genetic diversity","author":"Wu","year":"2018"},{"key":"ref22","first-page":"299","volume-title":"Methicillin-resistant coagulase-negative staphylococci on pig farms as a reservoir of heterogeneous staphylococcal cassette chromosome <italic>mec<\/italic> elements","author":"Tulinski","year":"2012"},{"key":"ref23","first-page":"2866","volume-title":"Enterotoxin genes in coagulase-negative and coagulase-positive staphylococci isolated from bovine milk","author":"de Freitas Guimaraes","year":"2013"},{"key":"ref24","first-page":"e02302","volume-title":"Evolutionary origin of the staphylococcal cassette chromosome <italic>mec<\/italic> (SCC<italic>mec<\/italic>)","author":"Rolo","year":"2017"},{"key":"ref25","first-page":"841","volume-title":"Distribution of antibiotic resistance genes among <italic>Staphylococcus<\/italic> species isolated from ready-to-eat foods","author":"Wang","year":"2019"},{"key":"ref26","first-page":"182","volume-title":"<italic>Staphylococcus aureus<\/italic> is more prevalent in retail beef livers than in pork and other beef cuts","author":"Abdalrahman","year":"2015"},{"key":"ref27","first-page":"231","volume-title":"Nasal carriage of methicillin-resistant coagulase-negative staphylococci in healthy humans is associated with occupational pig contact in a dose-response manner","author":"Li","year":"2017"},{"key":"ref28","first-page":"88","volume-title":"Heavy metal and disinfectant resistance genes among livestock-associated methicillin-resistant <italic>Staphylococcus aureus<\/italic> isolates","author":"Argudin","year":"2016"},{"key":"ref29","first-page":"401","volume-title":"Occurrence and characteristics of methicillin-resistant and -susceptible <italic>Staphylococcus aureus<\/italic> isolated from the beef production chain in Korea","author":"Lee","year":"2020"},{"key":"ref30","first-page":"S32","volume-title":"Resistance to zinc and cadmium in <italic>Staphylococcus aureus<\/italic> of human and animal origin","author":"Nair","year":"2014"},{"key":"ref31","first-page":"489","volume-title":"Zinc-resistance gene <italic>czrC<\/italic> identified in methicillin-resistant <italic>Staphylococcus hyicus<\/italic> isolated from pigs with exudative epidermitis","author":"Slifierz","year":"2014"},{"key":"ref32","first-page":"192","volume-title":"Dissemination of metal resistance genes among animal methicillin-resistant coagulase-negative staphylococci","author":"Argudin","year":"2016"},{"key":"ref33","first-page":"381","volume-title":"Detection of enterotoxin and toxic shock syndrome toxin 1 genes in <italic>Staphylococcus<\/italic>, with emphasis on coagulase-negative staphylococci","author":"de Lourdes","year":"2007"},{"key":"ref34","first-page":"580","volume-title":"The formation of <italic>Staphylococcus aureus<\/italic> enterotoxin in food environments and advances in risk assessment","author":"Schelin","year":"2011"},{"key":"ref35","first-page":"229","volume-title":"Staphylococcal enterotoxin synthesis during the exponential, transitional, and stationary growth phases","author":"Czop","year":"1974"},{"key":"ref36","first-page":"139","volume-title":"Evaluation of the spoilage potential of bacteria isolated from chilled chicken <italic>in vitro<\/italic> and <italic>in situ<\/italic>","author":"Wang","year":"2017"}],"container-title":["Animal 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