{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T05:24:47Z","timestamp":1774502687591,"version":"3.50.1"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2013,3,1]],"date-time":"2013-03-01T00:00:00Z","timestamp":1362096000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Two stability-indicating chromatographic methods are described for simultaneous determination of amiloride hydrochloride (AMI), atenolol (ATE), and chlorthalidone (CHL) in combined dosage forms. The first method was based on HPTLC separation of the three drugs followed by densitometric measurements of their bands at 274 nm. The separation was carried out on Merck HPTLC silica gel 60F254 aluminum sheets using chloroform\u2013methanol\u2013ammonia 27%, w\/w (9 + 2 + 0.3, v\/v\/v) mobile phase. Analysis data was used for the linear regression graph in the range of 0.1\u20130.5, 0.8\u20135.0, and 0.3\u20131.5 \u03bcg\/band for AMI, ATE, and CHL, respectively. The second method was based on an RP-HPLC separation of the cited drugs performed on an RP stainless steel C18 analytical column (250 \u00d7 4.6 mm id) with a gradient elution system of methanol and 0.05 M aqueous phosphate buffer adjusted to pH 4 as the mobile phase, at the flow rate of 1.0 mL\/min. Quantitation was achieved with photodiode array detection at 275 nm for AMI and 225 nm for ATE and CHL. The calibration graphs for each drug were rectilinear in the range of 2\u201350, 25\u2013150, and 2\u2013100 \u03bcg\/mL for AMI, ATE, and CHL, respectively. The proposed chromatographic methods were successfully applied for determination of the investigated drugs in pharmaceutical preparations. Both methods were validated in compliance with International Conference on Harmonization guidelines in terms of linearity, accuracy, precision, robustness, LOD, and LOQ.<\/jats:p>","DOI":"10.5740\/jaoacint.11-347","type":"journal-article","created":{"date-parts":[[2013,4,15]],"date-time":"2013-04-15T11:38:21Z","timestamp":1366025901000},"page":"313-323","source":"Crossref","is-referenced-by-count":20,"title":["Validated Stability-Indicating Methods for the Simultaneous Determination of Amiloride Hydrochloride, Atenolol, and Chlorthalidone Using HPTLC and HPLC with Photodiode Array Detector"],"prefix":"10.1093","volume":"96","author":[{"given":"Rasha M","family":"Youssef","sequence":"first","affiliation":[{"name":"University of Alexandria, Faculty of Pharmacy, Department of Pharmaceutical Analytical Chemistry, El-Messalah, Alexandria 21521, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hadir M","family":"Maher","sequence":"additional","affiliation":[{"name":"University of Alexandria, Faculty of Pharmacy, Department of Pharmaceutical Analytical Chemistry, El-Messalah, Alexandria 21521, Egypt King Saud University, Department of Pharmaceutical Chemistry, College of Pharmacy, Riyadh 11451, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eman I","family":"El-Kimary","sequence":"additional","affiliation":[{"name":"University of Alexandria, Faculty of Pharmacy, Department of Pharmaceutical Analytical Chemistry, El-Messalah, Alexandria 21521, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ekram M","family":"Hassan","sequence":"additional","affiliation":[{"name":"University of Alexandria, Faculty of Pharmacy, Department of Pharmaceutical Analytical Chemistry, El-Messalah, Alexandria 21521, Egypt Beirut Arab University, Faculty of Pharmacy, Department of Pharmaceutical Analytical Chemistry and Quality Control, Riad El Solh 110 72809, Beirut, Lebanon"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Magda H","family":"Barary","sequence":"additional","affiliation":[{"name":"University of Alexandria, Faculty of Pharmacy, Department of Pharmaceutical Analytical Chemistry, El-Messalah, Alexandria 21521, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2019,11,27]]},"container-title":["Journal of AOAC INTERNATIONAL"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/jaoac\/article-pdf\/96\/2\/313\/32424200\/jaoac0313.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/jaoac\/article-pdf\/96\/2\/313\/32424200\/jaoac0313.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,3,19]],"date-time":"2020-03-19T18:11:08Z","timestamp":1584641468000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jaoac\/article\/96\/2\/313-323\/5654912"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,3,1]]},"references-count":0,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2019,11,27]]},"published-print":{"date-parts":[[2013,3,1]]}},"URL":"https:\/\/doi.org\/10.5740\/jaoacint.11-347","relation":{},"ISSN":["1060-3271","1944-7922"],"issn-type":[{"value":"1060-3271","type":"print"},{"value":"1944-7922","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2013,3,1]]},"published":{"date-parts":[[2013,3,1]]}}}