{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,11]],"date-time":"2024-08-11T20:31:33Z","timestamp":1723408293611},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2018,5,1]],"date-time":"2018-05-01T00:00:00Z","timestamp":1525132800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>A new chromatographic-densitometric method has been developed for the qualitative and quantitative determination of the active ingredients in a simulated mixture corresponding to the PolyIran polypill, composed of acetylsalicylic acid, hydrochlorothiazide (HCT), enalapril (ENA), and atorvastatin (ATR), whose efficacy in the treatment and prevention of cardiovascular disease has been documented in clinical trials. Chromatographic separation was performed using TLC silica gel 60 plates with fluorescent indicator F254 as the stationary phase and a mixture of n-hexane\u2013ethyl acetate\u2013methanol\u2013water\u2013acetic acid (8.4 + 8 + 3 + 0.4 + 0.2, v\/v\/v\/v\/v) as the mobile phase. Densitometric measurements were carried out at \u03bb = 210 nm when determining ENA and at \u03bb = 265 nm in the case of the other drugs. Peaks of examined substances were well separated in the recorded chromatograms, enabling the evaluation of the results in terms of both qualitative and quantitative analysis. The method was specific for the analyzed components and was characterized by high sensitivity. The LOD was between 0.043 and 0.331 \u03bcg\/spot, and LOQ was between 0.100 and 0.942 \u03bcg\/spot. Recovery was in the range of 97.02\u2013101.34%. The linearity range was broad and ranged from 0.600 to 6.000 \u03bcg\/spot for acetylsalicylic acid, from 0.058 to 1.102 \u03bcg\/spot for HCT, from 0.505 to 6.560 \u03bcg\/spot for ENA, and from 0.100 to 1.000 \u03bcg\/spot for ATR. The method was characterized by good precision, with RSD values that ranged from 0.10 to 2.26%.<\/jats:p>","DOI":"10.5740\/jaoacint.17-0107","type":"journal-article","created":{"date-parts":[[2017,9,8]],"date-time":"2017-09-08T01:09:57Z","timestamp":1504832997000},"page":"708-713","source":"Crossref","is-referenced-by-count":1,"title":["Simultaneous Determination of Acetylsalicylic Acid, Hydrochlorothiazide, Enalapril, and Atorvastatin in a Polypill-Based Quaternary Mixture by TLC"],"prefix":"10.1093","volume":"101","author":[{"given":"Anna","family":"Ma\u015blanka","sequence":"first","affiliation":[{"name":"Jagiellonian University Medical College, Faculty of Pharmacy, Department of Inorganic and Analytical Chemistry, 9 Medyczna St, 30-688 Cracow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariusz","family":"Stolarczyk","sequence":"additional","affiliation":[{"name":"Jagiellonian University Medical College, Faculty of Pharmacy, Department of Inorganic and Analytical Chemistry, 9 Medyczna St, 30-688 Cracow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Apola","sequence":"additional","affiliation":[{"name":"Jagiellonian University Medical College, Faculty of Pharmacy, Department of Inorganic and Analytical Chemistry, 9 Medyczna St, 30-688 Cracow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Kwiecie\u0144","sequence":"additional","affiliation":[{"name":"Jagiellonian University Medical College, Faculty of Pharmacy, Department of Inorganic and Analytical Chemistry, 9 Medyczna St, 30-688 Cracow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Urszula","family":"Hubicka","sequence":"additional","affiliation":[{"name":"Jagiellonian University Medical College, Faculty of Pharmacy, Department of Inorganic and Analytical Chemistry, 9 Medyczna St, 30-688 Cracow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W\u0142odzimierz","family":"Opoka","sequence":"additional","affiliation":[{"name":"Jagiellonian University Medical College, Faculty of Pharmacy, Department of Inorganic and Analytical Chemistry, 9 Medyczna St, 30-688 Cracow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2019,11,23]]},"container-title":["Journal of AOAC INTERNATIONAL"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/jaoac\/article-pdf\/101\/3\/708\/32427091\/jaoac0708.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/jaoac\/article-pdf\/101\/3\/708\/32427091\/jaoac0708.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,3,19]],"date-time":"2020-03-19T18:17:08Z","timestamp":1584641828000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jaoac\/article\/101\/3\/708-713\/5654003"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,5,1]]},"references-count":0,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2019,11,23]]},"published-print":{"date-parts":[[2018,5,1]]}},"URL":"https:\/\/doi.org\/10.5740\/jaoacint.17-0107","relation":{},"ISSN":["1060-3271","1944-7922"],"issn-type":[{"value":"1060-3271","type":"print"},{"value":"1944-7922","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2018,5,1]]},"published":{"date-parts":[[2018,5,1]]}}}