{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T14:56:16Z","timestamp":1770908176109,"version":"3.50.1"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2020,2,11]],"date-time":"2020-02-11T00:00:00Z","timestamp":1581379200000},"content-version":"vor","delay-in-days":347,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Background: Oxyclozanide (OXY) is a veterinary medicine used for control of fascioliasis in farm animals. Literature review shows absence of sufficient information regarding its stability. Such information is important as it affects many stages of a drug\u2019s life cycle, from pharmaceutical manufacturing to its environmental fate understanding of the degradation of the drug once itis placed in the environment. Objective: An HPLC method was developed to address the impact of different stress conditions on OXY\u2019s stability. Methods: OXY\u2019s stability was investigated by exposure to forced acid and alkaline hydrolysis, thermal, oxidative and photolytic degradation, which are different stress conditions applied to the forced degradation study. Separation was performed on Eurosphere C18 analytical column (125 \u00d7 4.6 mm, 5 \u03bcm particle size) using 50 mM sodium acetate trihydrate (pH 4.5) and acetonitrile (50:50, v\/v) as mobile phase and UV detection at 254 nm. A photolytic kineticsstudy was conducted by monitoring OXY photolysis under monochromatic and polychromatic light sources (UV lamp at 366 nm and natural sunlight) in aqueous buffers of different pHs (5, 7, and 9). LC-MS was used to identify the major photolytic degradate. Results: OXY was quantified over a concentration range of 1\u201380 \u03bcg\/mL with mean recovery of 99.32 \u00b1 1.80%. The drug wassusceptible to oxidative and photolytic degradation. The photolytic kinetics were pH dependent. The LC-MS result supported photo-dehalogenation degradation mechanism. Conclusions: The developed method could be used in OXY stability testing. The results of the photolytic kinetics study canaddress OXY aquatic photo-transformation, thereby predicting its environmental fate and risks imposed on the ecosystem. Highlights: An HPLC method was developed for monitoring OXY degradation behavior and studying its photolytic kinetics with identification of its photodegradate.<\/jats:p>","DOI":"10.5740\/jaoacint.17-0364","type":"journal-article","created":{"date-parts":[[2018,8,8]],"date-time":"2018-08-08T01:09:06Z","timestamp":1533690546000},"page":"480-489","source":"Crossref","is-referenced-by-count":5,"title":["Study of Oxyclozanide\u2019s Innate Stability Coupled with the Assessment of its Aquatic Photo-Transformation Using a Validated Isocratic HPLC Method"],"prefix":"10.1093","volume":"102","author":[{"given":"Ahmed S","family":"Saad","sequence":"first","affiliation":[{"name":"Cairo University, Faculty of Pharmacy, Analytical Chemistry Department, Kasr El-Aini St, Cairo, Egypt 11562"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nahla S","family":"Ismail","sequence":"additional","affiliation":[{"name":"National Organization of Drug Control and Research, 51 Wezzart Elzeraa St, Elagouza, Giza, Egypt 12654"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marwa","family":"Soliman","sequence":"additional","affiliation":[{"name":"National Organization of Drug Control and Research, 51 Wezzart Elzeraa St, Elagouza, Giza, Egypt 12654"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hala E","family":"Zaazaa","sequence":"additional","affiliation":[{"name":"Cairo University, Faculty of Pharmacy, Analytical Chemistry Department, Kasr El-Aini St, Cairo, Egypt 11562"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2019,11,23]]},"container-title":["Journal of AOAC INTERNATIONAL"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/jaoac\/article-pdf\/102\/2\/480\/36326015\/jaoac0480.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/jaoac\/article-pdf\/102\/2\/480\/36326015\/jaoac0480.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,2,20]],"date-time":"2021-02-20T05:48:18Z","timestamp":1613800098000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jaoac\/article\/102\/2\/480\/5658148"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3,1]]},"references-count":0,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2019,11,23]]},"published-print":{"date-parts":[[2019,3,1]]}},"URL":"https:\/\/doi.org\/10.5740\/jaoacint.17-0364","relation":{},"ISSN":["1060-3271","1944-7922"],"issn-type":[{"value":"1060-3271","type":"print"},{"value":"1944-7922","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2019,3,1]]},"published":{"date-parts":[[2019,3,1]]}}}