{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T22:45:22Z","timestamp":1793313922425,"version":"4.5.0"},"reference-count":0,"publisher":"ScopeMed","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Microbil Infect Dis"],"accepted":{"date-parts":[[2023,2,18]]},"abstract":"<jats:p xml:lang=\"en\">Objectives: The use of bacteriophages (phages) as an alternative treatment for multidrug-resistant bacteria has recently gained popularity. Phage cocktails have been proposed for broad-spectrum therapeutic effects against such resistant bacteria. However, the effectiveness of non-locally formulated phage cocktails for therapy on Kenyan isolates has yet to be evaluated and is a subject of investigation. This study aimed to determine the in vitro effectiveness of a non-locally made commercial pyophage cocktail on clinical Pseudomonas aeruginosa isolates from Kenya.  \r\nMethodology: Forty-nine P. aeruginosa isolates from Kenya were subjected to a pyophage cocktail for efficacy studies using direct spot test (DST) and efficiency of plating (EOP).\r\nResults: The success rate of the cocktail was observed on 16.3% (8\/49) isolates only and ineffective on 83.7% (41\/49) isolates. Six of the eight isolates that showed cross-reactivity from DST had complete lysis with a faintly hazy background. Five of these six isolates resulted in successful and high phage progeny production in plaquing efficiency (EOP \u2265 0.5).\r\nConclusion: Non-locally made commercial pyophage cocktail was ineffective against the 83.7% endemic clinical strains of the Kenyan P. aeruginosa isolates, demonstrating the importance of locally derived phage cocktails against endemic and multidrug-resistant isolates. J Microbiol Infect Dis 2023; 12(4):25-30.<\/jats:p>","DOI":"10.5799\/jmid.1265378","type":"journal-article","created":{"date-parts":[[2023,3,14]],"date-time":"2023-03-14T21:01:22Z","timestamp":1678827682000},"page":"25-30","source":"Crossref","is-referenced-by-count":3,"title":["Evaluating The Effectiveness of A Non-Locally Developed Commercial Phage Cocktail on Kenyan Pseudomonas aeruginosa Isolates"],"prefix":"10.5455","author":[{"given":"Ivy Jepkurui","family":"MUTAIL","sequence":"first","affiliation":[{"name":"Kenya Medical Research Institute, Nairobi, Kenya"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"GEORGES","sequence":"additional","affiliation":[{"name":"Kenya Medical Research Institute, Nairobi, Kenya"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atunga","family":"NYACHIEO","sequence":"additional","affiliation":[{"name":"Kenya Medical Research Institute, Nairobi, Kenya"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"ODOYO","sequence":"additional","affiliation":[{"name":"Kenya Medical Research Institute, Nairobi, Kenya"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony Kebira","family":"NYAMACHE","sequence":"additional","affiliation":[{"name":"Kenya Medical Research Institute, Nairobi, Kenya"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jillani","family":"NGALLA","sequence":"additional","affiliation":[{"name":"Kenya Medical Research Institute, Nairobi, Kenya"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3407","published-online":{"date-parts":[[2023,3,15]]},"container-title":["Journal of Microbiology and Infectious Diseases"],"original-title":[],"language":"en","deposited":{"date-parts":[[2023,5,3]],"date-time":"2023-05-03T14:54:52Z","timestamp":1683125692000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jmidonline.org\/?mno=302657062"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,15]]},"references-count":0,"URL":"https:\/\/doi.org\/10.5799\/jmid.1265378","relation":{},"ISSN":["2146-3158"],"issn-type":[{"value":"2146-3158","type":"print"}],"subject":[],"published":{"date-parts":[[2023,3,15]]}}}