{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T05:50:49Z","timestamp":1787032249313,"version":"build-2736575974"},"reference-count":52,"publisher":"Brieflands","issue":"1","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["brieflands.com"],"crossmark-restriction":false},"short-container-title":["Iran J Pharm Res"],"abstract":"<jats:p>: Cytotoxic activity of crude extract and fractions (petroleum ether, dichloromethane, and n-butanol) of Artemisia haussknechtii aerial parts was investigated by MTT assay. Dichloromethane fraction showed the highest cytotoxic effect on MCF-7 cell line (IC50 = 297.17 \u00b1 7.99 \u00b5g\/mL). Phytochemical analysis of the most effective fraction was carried out using normal phase column chromatography (CC) to get eight sub-fractions (A-H). Thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) were used for further purification. Four known compounds with cytotoxic effects on cancer cell lines were isolated from the most active fraction, including 5-Hydroxy-3',4',6,7-tetramethoxyflavone (eupatilin 7-methyl ether), 5-hydroxy 3,3',4',6,7-pentamethoxy-flavone (artemetin), 6-methoxy-7-hydroxycoumarin (scopoletin), and methyl caffeate. Structure elucidation of isolated compounds was done using spectroscopic techniques, including ESIMS and 1D-NMR (1H and 13C). Cytotoxic activity of A. haussknechtii is probably due to coumarin and flavonoid compounds.<\/jats:p>","DOI":"10.5812\/ijpr-126917","type":"journal-article","created":{"date-parts":[[2022,4,30]],"date-time":"2022-04-30T02:32:44Z","timestamp":1651285964000},"update-policy":"https:\/\/doi.org\/10.5812\/crossmark_update_policy","source":"Crossref","is-referenced-by-count":8,"title":["Cytotoxic Activity and Phytochemical Analysis of Artemisia haussknechtii Boiss"],"prefix":"10.5812","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5818-1324","authenticated-orcid":false,"given":"Sajjad","family":"Nasseri","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad-Reza","family":"Delnavazi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farshad H.","family":"Shirazi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2415-2175","authenticated-orcid":false,"given":"Faraz","family":"Mojab","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3819","published-online":{"date-parts":[[2022,4,30]]},"reference":[{"key":"key-A126917REF1-1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2148-2-17"},{"key":"key-A126917REF2-2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2019.115180"},{"issue":"1","key":"key-A126917REF3-3","first-page":"391","volume":"18","author":"Hosseinzadeh\u00a0L","year":"2019","journal-title":"Iran J Pharm Res."},{"key":"key-A126917REF4-4","doi-asserted-by":"publisher","DOI":"10.1055\/s-0034-1383146"},{"key":"key-A126917REF5-5","doi-asserted-by":"publisher","DOI":"10.1055\/s-0034-1372647"},{"key":"key-A126917REF6-6","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2016.3556"},{"issue":"2","key":"key-A126917REF7-7","first-page":"155","volume":"9","author":"Nikbakht\u00a0MR","year":"2014","journal-title":"Res Pharm Sci."},{"issue":"3","key":"key-A126917REF8-8","first-page":"337","volume":"35","author":"Li\u00a0Y","year":"2004","journal-title":"Sichuan Da Xue Xue Bao Yi Xue Ban."},{"issue":"12","key":"key-A126917REF9-9","first-page":"1988","volume":"36","author":"Zhang\u00a0LM","year":"2013","journal-title":"Zhong Yao Cai."},{"key":"key-A126917REF10-10","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6882-14-226"},{"key":"key-A126917REF11-11","doi-asserted-by":"publisher","DOI":"10.22038\/ijbms.2017.8242"},{"key":"key-A126917REF12-12","doi-asserted-by":"publisher","DOI":"10.7314\/apjcp.2014.15.17.7055"},{"key":"key-A126917REF13-13","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/628073"},{"key":"key-A126917REF14-14","doi-asserted-by":"publisher","DOI":"10.1590\/s0102-695x2013000500010"},{"key":"key-A126917REF15-15","doi-asserted-by":"publisher","DOI":"10.4103\/1735-5362.288434"},{"key":"key-A126917REF16-16","first-page":"778","volume":"2","author":"Mahmoudi\u00a0M","year":"2009","journal-title":"Pharmacologyonline."},{"key":"key-A126917REF17-17","first-page":"448","volume":"59","author":"Mozaffarian\u00a0V","year":"2008","journal-title":"Flora of Iran. 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