{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,13]],"date-time":"2026-09-13T20:25:03Z","timestamp":1789331103682,"version":"build-2803163510"},"reference-count":27,"publisher":"Brieflands","issue":"1","license":[{"start":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T00:00:00Z","timestamp":1759968000000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["brieflands.com"],"crossmark-restriction":false},"short-container-title":["Iran J Pharm Res"],"abstract":"<jats:p>Background: MicroRNAs (miRNAs) play key roles in colorectal cancer (CRC) progression and metastasis. miR-182-5p acts as an oncogenic metastamiR, frequently upregulated in cancers and promoting cell migration and invasion. In contrast, miR-203, miR-150-5p, and miR-139-5p function as tumor suppressors and are often downregulated in CRC. Methods: Expression levels of these four miRNAs were quantified in three CRC cell lines using real-time polymerase chain reaction (RT-PCR). Functional assays, including cell viability, migration, and invasion, were conducted after silencing miR-182-5p or overexpressing the tumor-suppressive miRNAs through mimic transfection. Results: The miR-182-5p was significantly overexpressed and positively correlated with metastatic potential, while miR-203, miR-150-5p, and miR-139-5p were downregulated and inversely associated with metastatic traits. Modulation of these miRNAs reduced CRC cell viability, migration, and invasion. Mechanistically, miR-182-5p enhanced metastasis via ANLN and PDE4D regulation, whereas miR-203, miR-150-5p, and miR-139-5p suppressed metastasis through PDE4D, NEGR1, and ATP11A pathways, respectively. Conclusions: These results highlight the opposing roles of miR-182-5p and the tumor-suppressive miRNAs in CRC metastasis and suggest their potential as biomarkers and therapeutic targets.<\/jats:p>","DOI":"10.5812\/ijpr-164911","type":"journal-article","created":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T09:05:19Z","timestamp":1765962319000},"update-policy":"https:\/\/doi.org\/10.5812\/crossmark_update_policy","source":"Crossref","is-referenced-by-count":1,"title":["Oncogenic Function of miR-182-5p Versus Tumor-Suppressive Activities of miR-203, miR-150-5p, and miR-139-5p via Target Gene Regulation in Colon Cancer Metastasis"],"prefix":"10.5812","volume":"24","author":[{"given":"Sheryar","family":"Afzal","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Attiq","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3819","published-online":{"date-parts":[[2025,11,23]]},"reference":[{"key":"key-A164911REF1-1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21660"},{"key":"key-A164911REF2-2","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21763"},{"key":"key-A164911REF3-3","doi-asserted-by":"publisher","DOI":"10.1016\/s0092-8674(04)00045-5"},{"key":"key-A164911REF4-4","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1997"},{"key":"key-A164911REF5-5","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1840"},{"key":"key-A164911REF6-6","doi-asserted-by":"publisher","DOI":"10.1007\/s00384-013-1674-0"},{"key":"key-A164911REF7-7","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0808263106"},{"key":"key-A164911REF8-8","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.24071"},{"key":"key-A164911REF9-9","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-11-3864"},{"key":"key-A164911REF10-10","doi-asserted-by":"publisher","DOI":"10.1038\/srep28301"},{"key":"key-A164911REF11-11","doi-asserted-by":"publisher","DOI":"10.7314\/apjcp.2014.15.15.6269"},{"key":"key-A164911REF12-12","doi-asserted-by":"publisher","DOI":"10.1016\/j.prp.2016.04.011"},{"key":"key-A164911REF13-13","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.31062"},{"key":"key-A164911REF14-14","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-023-08810-w"},{"key":"key-A164911REF15-15","doi-asserted-by":"publisher","DOI":"10.26355\/eurrev_201902_17107"},{"key":"key-A164911REF16-16","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2011-301846"},{"key":"key-A164911REF17-17","doi-asserted-by":"publisher","DOI":"10.1016\/j.ctarc.2022.100647"},{"key":"key-A164911REF18-18","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1205802"},{"key":"key-A164911REF19-19","doi-asserted-by":"publisher","DOI":"10.3390\/cancers12061600"},{"key":"key-A164911REF20-20","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-80735-x"},{"key":"key-A164911REF21-21","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0154130"},{"key":"key-A164911REF22-22","doi-asserted-by":"publisher","DOI":"10.1186\/1479-5876-9-197"},{"issue":"12","key":"key-A164911REF23-23","first-page":"2387","volume":"8","author":"Chen\u00a0L","year":"2018","journal-title":"Am J Cancer Res."},{"key":"key-A164911REF24-24","doi-asserted-by":"publisher","DOI":"10.1080\/02699052.2024.2373914"},{"key":"key-A164911REF25-25","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2017.6351"},{"issue":"2","key":"key-A164911REF26-26","first-page":"505","volume":"23","author":"Miyoshi\u00a0N","year":"2010","journal-title":"Oncol Rep."},{"key":"key-A164911REF27-27","doi-asserted-by":"publisher","DOI":"10.1007\/s10495-014-0999-7"}],"container-title":["Iranian Journal of Pharmaceutical Research"],"original-title":[],"link":[{"URL":"https:\/\/brieflands.com\/journals\/ijpr\/articles\/164911","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/brieflands.com\/journals\/ijpr\/articles\/164911","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T09:05:21Z","timestamp":1765962321000},"score":1,"resource":{"primary":{"URL":"https:\/\/brieflands.com\/journals\/ijpr\/articles\/164911"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,23]]},"references-count":27,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,11,23]]}},"alternative-id":["1afe498b039526be4e06adf80559580fa0e35652"],"URL":"https:\/\/doi.org\/10.5812\/ijpr-164911","relation":{},"ISSN":["1726-6890"],"issn-type":[{"value":"1726-6890","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,23]]}}}