{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T17:59:52Z","timestamp":1783447192066,"version":"3.55.0"},"reference-count":33,"publisher":"Brieflands","issue":"1","license":[{"start":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T00:00:00Z","timestamp":1773187200000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["brieflands.com"],"crossmark-restriction":false},"short-container-title":["Iran J Pharm Res"],"abstract":"<jats:p>Background: Clerodendrum chinense has been reported to possess several important biological activities. However, the anti-inflammation and insulin-related properties of isolated compounds from this plant have not yet been investigated. Objectives: This study focuses on extracting compounds and evaluating improvements in insulin resistance through the anti-inflammatory potential and inhibition of reactive oxygen species (ROS) of hispidulin from Vietnamese C. chinense leaves. Methods: Ethanol was used to separate the compounds from the leaves of Vietnamese C. chinense. 1D, 2D-NMR, and ESI-MS spectra were used to analyze the structural identity of the isolated compounds. Compound 1 (hispidulin) was evaluated for anti-inflammation and ROS in Raw 264.7 cells: Anti-inflammatory cytokine inhibition ability was analyzed by the ELISA method; Western blotting was used for the expression levels of phosphorylated ERK1\/2, p38, p47phox, and GLUT4. ROS was assessed by immunofluorescence; a luminometer device was used to measure NADPH oxidase activity. The ability to absorb glucose and counteract insulin resistance was evaluated in 3T3-L1 cells via insulin-induced glucose uptake using immunofluorescence dye. Results: One compound isolated from the leaves of Vietnamese C. chinense was identified as hispidulin. Hispidulin at a concentration of 50 \u03bcg\/mL significantly inhibited tumor necrosis factor (TNF)-\u03b1, interleukin (IL)-6, and IL-8 in LPS-induced macrophages (P &lt; 0.001). Moreover, in LPS-stimulated macrophages, the phosphorylation of p38 and ERK1\/2, NADPH oxidase activity, as well as the phosphorylation of p47phox, were reduced at a concentration of 50 \u03bcg\/mL of hispidulin (P &lt; 0.001). More importantly, 3T3-L1 cells with inhibited insulin activity were able to regain glucose uptake and insulin responsiveness through GLUT4 expression when treated with 50 \u00b5g\/mL of hispidulin (P &lt; 0.001). Conclusions: The data confirmed the presence of hispidulin in the chemical composition of Vietnamese C. chinense leaves. The results indicated that hispidulin inhibited LPS- and ROS-mediated inflammation in macrophages. More importantly, the data have shown that hispidulin enhances glucose uptake and improves insulin activity in 3T3-L1 cells.<\/jats:p>","DOI":"10.5812\/ijpr-169136","type":"journal-article","created":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T11:13:20Z","timestamp":1778498000000},"update-policy":"https:\/\/doi.org\/10.5812\/crossmark_update_policy","source":"Crossref","is-referenced-by-count":1,"title":["Anti-inflammation and Anti-insulin Resistance of a Compound from Vietnamese &lt;i&gt;Clerodendrum chinense&lt;\/i&gt; Leaves"],"prefix":"10.5812","volume":"25","author":[{"given":"Xuan","family":"Thi Xuan Nguyen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cuong","family":"Trinh 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