{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,3]],"date-time":"2025-12-03T19:48:19Z","timestamp":1764791299528,"version":"3.46.0"},"reference-count":10,"publisher":"Archives of Pathology and Laboratory Medicine","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2000,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Cross-contamination during sequential processing of sputum specimens from different patients causes false-positive growth of Mycobacterium tuberculosis in culture. We describe an unusual case of cross-contamination in a 36-year-old man with acquired immunodeficiency syndrome and possible persistent tuberculosis. Culture with 1 of 3 sputum specimens was positive for rifampin-susceptible M tuberculosis. Review of processing revealed that his single culture-positive sputum specimen had followed a sputum specimen from another patient with active pulmonary tuberculosis that was positive in culture for M tuberculosis resistant to rifampin. Molecular strain typing by restriction fragment length polymorphism demonstrated the 2 isolates to be an identical strain of M tuberculosis. Agar proportion susceptibility testing of the rifampin-resistant isolate revealed low numbers of resistant organisms in a range of 1.5% to 3.3%. It was concluded that rifampin-susceptible organisms that constituted approximately 98% of the resistant isolate contaminated sputum from the patient with possible persistent tuberculosis. His culture result was, therefore, considered false positive, not an indication of tuberculosis.<\/jats:p>","DOI":"10.5858\/2000-124-0902-ptosc","type":"journal-article","created":{"date-parts":[[2021,2,10]],"date-time":"2021-02-10T10:38:43Z","timestamp":1612953523000},"page":"902-903","source":"Crossref","is-referenced-by-count":1,"title":["Persistent Tuberculosis or Specimen Contamination?"],"prefix":"10.5858","volume":"124","author":[{"given":"Kathy","family":"Trakas","sequence":"first","affiliation":[{"name":"From the Department of Pathology, Northwestern University Medical School, Chicago, Ill (Ms Trakas and Drs Stosor and Warren), and the Molecular Epidemiology Unit of the Michigan Department of Community Health, Lansing, Mich (Mr Dietrich and Ms Mosher)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valentina","family":"Stosor","sequence":"additional","affiliation":[{"name":"From the Department of Pathology, Northwestern University Medical School, Chicago, Ill (Ms Trakas and Drs Stosor and Warren), and the Molecular Epidemiology Unit of the Michigan Department of Community Health, Lansing, Mich (Mr Dietrich and Ms Mosher)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen","family":"Dietrich","sequence":"additional","affiliation":[{"name":"From the Department of Pathology, Northwestern University Medical School, Chicago, Ill (Ms Trakas and Drs Stosor and Warren), and the Molecular Epidemiology Unit of the Michigan Department of Community Health, Lansing, Mich (Mr Dietrich and Ms Mosher)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Mosher","sequence":"additional","affiliation":[{"name":"From the Department of Pathology, Northwestern University Medical School, Chicago, Ill (Ms Trakas and Drs Stosor and Warren), and the Molecular Epidemiology Unit of the Michigan Department of Community Health, Lansing, Mich (Mr Dietrich and Ms Mosher)."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John R.","family":"Warren","sequence":"additional","affiliation":[{"name":"Reprints: John R. Warren, MD, Department of Pathology, Northwestern University Medical School, 303 E Chicago Ave, Chicago, IL 60611 (e-mail: jwarren@nmh.org)."},{"name":"From the Department of Pathology, Northwestern University Medical School, Chicago, Ill (Ms Trakas and Drs Stosor and Warren), and the Molecular Epidemiology Unit of the Michigan Department of Community Health, Lansing, Mich (Mr Dietrich and Ms Mosher)."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"3906","reference":[{"key":"2021020915104209900_i1543-2165-124-6-902-b1","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1093\/ajcp\/109.3.324","article-title":"Cross-contamination of specimens with Mycobacterium tuberculosis: clinical significance, causes, and prevention.","volume":"109","author":"Bhattacharya","year":"1998","journal-title":"Am J Clin Pathol"},{"key":"2021020915104209900_i1543-2165-124-6-902-b2","doi-asserted-by":"crossref","first-page":"1677","DOI":"10.1128\/JCM.31.7.1677-1682.1993","article-title":"Molecular strain typing of Mycobacterium tuberculosis to confirm cross-contamination in the mycobacteriology laboratory and modification of procedures to minimize occurrence of false-positive cultures.","volume":"31","author":"Small","year":"1993","journal-title":"J Clin Microbiol"},{"key":"2021020915104209900_i1543-2165-124-6-902-b3","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1128\/JCM.34.4.1017-1019.1996","article-title":"Specimen contamination in mycobacteriology laboratory detected by pseudo-outbreak of multidrug-resistant tuberculosis: analysis by routine epidemiology and confirmation by molecular technique.","volume":"34","author":"Wurtz","year":"1996","journal-title":"J Clin Microbiol"},{"key":"2021020915104209900_i1543-2165-124-6-902-b4","doi-asserted-by":"crossref","first-page":"988","DOI":"10.1128\/JCM.35.4.988-991.1997","article-title":"False-positive results from cultures of Mycobacterium tuberculosis due to laboratory cross-contamination confirmed by restriction fragment length polymorphism.","volume":"35","author":"Bauer","year":"1997","journal-title":"J Clin Microbiol"},{"key":"2021020915104209900_i1543-2165-124-6-902-b5","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1093\/clinids\/24.1.35","article-title":"Retrospective detection of laboratory cross-contamination of Mycobacterium tuberculosis cultures with use of DNA fingerprint analysis.","volume":"24","author":"Braden","year":"1997","journal-title":"Clin Infect Dis"},{"key":"2021020915104209900_i1543-2165-124-6-902-b6","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1164\/ajrccm.155.1.9001331","article-title":"The incidence of false-positive cultures for Mycobacterium tuberculosis.","volume":"155","author":"Burman","year":"1997","journal-title":"Am J Respir Crit Care Med"},{"key":"2021020915104209900_i1543-2165-124-6-902-b7","doi-asserted-by":"crossref","first-page":"1702","DOI":"10.1164\/ajrccm.152.5.7582316","article-title":"Laboratory contamination of Mycobacterium tuberculosis cultures.","volume":"152","author":"Dunlap","year":"1995","journal-title":"Am J Respir Crit Care Med"},{"key":"2021020915104209900_i1543-2165-124-6-902-b8","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1128\/JCM.31.2.406-409.1993","article-title":"Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a standardized methodology.","volume":"31","author":"Van Embden","year":"1993","journal-title":"J Clin Microbiol"},{"key":"2021020915104209900_i1543-2165-124-6-902-b9","doi-asserted-by":"crossref","first-page":"1118","DOI":"10.1128\/JCM.34.5.1118-1123.1996","article-title":"Usefulness of the secondary probe pTBN12 in DNA fingerprinting of Mycobacterium tuberculosis.","volume":"34","author":"Chaves","year":"1996","journal-title":"J Clin Microbiol"},{"key":"2021020915104209900_i1543-2165-124-6-902-b10","doi-asserted-by":"crossref","first-page":"1044","DOI":"10.1086\/516065","article-title":"Acquired drug resistance in Mycobacterium tuberculosis isolates recovered from compliant patients with human immunodeficiency virus-associated tuberculosis.","volume":"25","author":"March","year":"1997","journal-title":"Clin Infect Dis"}],"container-title":["Archives of Pathology &amp; Laboratory Medicine"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/meridian.allenpress.com\/aplm\/article-pdf\/124\/6\/902\/2751374\/0003-9985(2000)124_0902_ptosc_2_0_co_2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/meridian.allenpress.com\/aplm\/article-pdf\/124\/6\/902\/2751374\/0003-9985(2000)124_0902_ptosc_2_0_co_2.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,3]],"date-time":"2025-12-03T19:37:49Z","timestamp":1764790669000},"score":1,"resource":{"primary":{"URL":"https:\/\/aplm.kglmeridian.com\/view\/journals\/arpa\/124\/6\/article-p902.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,6,1]]},"references-count":10,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2000,6,1]]}},"URL":"https:\/\/doi.org\/10.5858\/2000-124-0902-ptosc","relation":{},"ISSN":["1543-2165","0003-9985"],"issn-type":[{"type":"electronic","value":"1543-2165"},{"type":"print","value":"0003-9985"}],"subject":[],"published":{"date-parts":[[2000,6,1]]}}}