{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,3]],"date-time":"2025-12-03T19:43:17Z","timestamp":1764790997754,"version":"3.46.0"},"reference-count":39,"publisher":"Archives of Pathology and Laboratory Medicine","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2002,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Context.\u2014Meningococcal infection has a high public profile because of its dramatic presentation, high fatality rate, and propensity to occur in outbreaks and clusters of cases. Use of a diagnostic polymerase chain reaction (PCR) assay could enhance laboratory confirmation of cases and guide the public health response in North America.<\/jats:p>\n                  <jats:p>Objective.\u2014To assess the performance of a PCR assay for the diagnosis of meningococcal disease after its implementation in a North American setting and to evaluate sensitivity and specificity of the assay for the detection of prevalent bacterial isolates.<\/jats:p>\n                  <jats:p>Design.\u2014Laboratory evaluation of the sensitivity and specificity of a PCR assay for Neisseria meningitidis and observational study of a series of cases comparing molecular diagnosis against the criterion standard of conventional laboratory diagnostic tests.<\/jats:p>\n                  <jats:p>Setting.\u2014A Canadian province with a population of 4 million people.<\/jats:p>\n                  <jats:p>Patients.\u2014Children and adults presenting with suspected meningococcal disease in British Columbia.<\/jats:p>\n                  <jats:p>Main Outcome Measures.\u2014The sensitivity and specificity of the PCR assay when compared against standard laboratory methods.<\/jats:p>\n                  <jats:p>Results.\u2014The PCR assay correctly identified all of 38 Canadian isolates of Neisseria meningitidis and correctly assigned the serogroup to each isolate. None of 57 other gram-positive or gram-negative bacteria or yeasts were detected by the PCR assay. In a clinical evaluation, for diagnosis of meningococcal disease, the PCR assay had a sensitivity and specificity of 91% and 76%, respectively, against conventional methods of diagnosis. Use of the PCR assay increased the laboratory confirmation of clinically suspected cases by 36%. During an outbreak, the PCR assay allowed serogroup determination in 3 of 7 cases, aiding in the public health decision to launch an immunization campaign.<\/jats:p>\n                  <jats:p>Conclusions.\u2014The PCR assay is more sensitive than conventional methods for the diagnosis of meningococcal disease, and enhanced surveillance may help direct the public health response to the changing epidemiology of disease in North America.<\/jats:p>","DOI":"10.5858\/2002-126-1209-eoadpc","type":"journal-article","created":{"date-parts":[[2021,1,29]],"date-time":"2021-01-29T12:18:43Z","timestamp":1611922723000},"page":"1209-1215","source":"Crossref","is-referenced-by-count":20,"title":["Evaluation of a Diagnostic Polymerase Chain Reaction Assay for\n                    <i>Neisseria meningitidis<\/i>\n                    in North America and Field Experience During an Outbreak"],"prefix":"10.5858","volume":"126","author":[{"given":"Andrew J.","family":"Pollard","sequence":"first","affiliation":[{"name":"From the Division of Infectious and Immunological Diseases, Department of Pediatrics, University of British Columbia, British Columbia's Research Institute for Child and Family Health, Vancouver, Canada (Drs Pollard and Speert, and Mr Probe); the Clinical Microbiology Laboratory, Department of Pathology, Children's and Women's Health Center of British Columbia, Vancouver, Canada (Mss Trombley, Ca"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gary","family":"Probe","sequence":"additional","affiliation":[{"name":"From the Division of Infectious and Immunological Diseases, Department of Pediatrics, University of British Columbia, British Columbia's Research Institute for Child and Family Health, Vancouver, Canada (Drs Pollard and Speert, and Mr Probe); the Clinical Microbiology Laboratory, Department of Pathology, Children's and Women's Health Center of British Columbia, Vancouver, Canada (Mss Trombley, Ca"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Colleen","family":"Trombley","sequence":"additional","affiliation":[{"name":"From the Division of Infectious and Immunological Diseases, Department of Pediatrics, University of British Columbia, British Columbia's Research Institute for Child and Family Health, Vancouver, Canada (Drs Pollard and Speert, and Mr Probe); the Clinical Microbiology Laboratory, Department of Pathology, Children's and Women's Health Center of British Columbia, Vancouver, Canada (Mss Trombley, Ca"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annette","family":"Castell","sequence":"additional","affiliation":[{"name":"From the Division of Infectious and Immunological Diseases, Department of Pediatrics, University of British Columbia, British Columbia's Research Institute for Child and Family Health, Vancouver, Canada (Drs Pollard and Speert, and Mr Probe); the Clinical Microbiology Laboratory, Department of Pathology, Children's and Women's Health Center of British Columbia, Vancouver, Canada (Mss Trombley, Ca"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sue","family":"Whitehead","sequence":"additional","affiliation":[{"name":"From the Division of Infectious and Immunological Diseases, Department of Pediatrics, University of British Columbia, British Columbia's Research Institute for Child and Family Health, Vancouver, Canada (Drs Pollard and Speert, and Mr Probe); the Clinical Microbiology Laboratory, Department of Pathology, Children's and Women's Health Center of British Columbia, Vancouver, Canada (Mss Trombley, Ca"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. Mark","family":"Bigham","sequence":"additional","affiliation":[{"name":"From the Division of Infectious and Immunological Diseases, Department of Pediatrics, University of British Columbia, British Columbia's Research Institute for Child and Family Health, Vancouver, Canada (Drs Pollard and Speert, and Mr Probe); the Clinical Microbiology Laboratory, Department of Pathology, Children's and Women's Health Center of British Columbia, Vancouver, Canada (Mss Trombley, Ca"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sylvie","family":"Champagne","sequence":"additional","affiliation":[{"name":"From the Division of Infectious and Immunological Diseases, Department of Pediatrics, University of British Columbia, British Columbia's Research Institute for Child and Family Health, Vancouver, Canada (Drs Pollard and Speert, and Mr Probe); the Clinical Microbiology Laboratory, Department of Pathology, Children's and Women's Health Center of British Columbia, Vancouver, Canada (Mss Trombley, Ca"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Judy","family":"Isaac-Renton","sequence":"additional","affiliation":[{"name":"From the Division of Infectious and Immunological Diseases, Department of Pediatrics, University of British Columbia, British Columbia's Research Institute for Child and Family Health, Vancouver, Canada (Drs Pollard and Speert, and Mr Probe); the Clinical Microbiology Laboratory, Department of Pathology, Children's and Women's Health Center of British Columbia, Vancouver, Canada (Mss Trombley, Ca"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rusung","family":"Tan","sequence":"additional","affiliation":[{"name":"From the Division of Infectious and Immunological Diseases, Department of Pediatrics, University of British Columbia, British Columbia's Research Institute for Child and Family Health, Vancouver, Canada (Drs Pollard and Speert, and Mr Probe); the Clinical Microbiology Laboratory, Department of Pathology, Children's and Women's Health Center of British Columbia, Vancouver, Canada (Mss Trombley, Ca"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Malcolm","family":"Guiver","sequence":"additional","affiliation":[{"name":"From the Division of Infectious and Immunological Diseases, Department of Pediatrics, University of British Columbia, British Columbia's Research Institute for Child and Family Health, Vancouver, Canada (Drs Pollard and Speert, and Mr Probe); the Clinical Microbiology Laboratory, Department of Pathology, Children's and Women's Health Center of British Columbia, Vancouver, Canada (Mss Trombley, Ca"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ray","family":"Borrow","sequence":"additional","affiliation":[{"name":"From the Division of Infectious and Immunological Diseases, Department of Pediatrics, University of British Columbia, British Columbia's Research Institute for Child and Family Health, Vancouver, Canada (Drs Pollard and Speert, and Mr Probe); the Clinical Microbiology Laboratory, Department of Pathology, Children's and Women's Health Center of British Columbia, Vancouver, Canada (Mss Trombley, Ca"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David P.","family":"Speert","sequence":"additional","affiliation":[{"name":"From the Division of Infectious and Immunological Diseases, Department of Pediatrics, University of British Columbia, British Columbia's Research Institute for Child and Family Health, Vancouver, Canada (Drs Pollard and Speert, and Mr Probe); the Clinical Microbiology Laboratory, Department of Pathology, Children's and Women's Health Center of British Columbia, Vancouver, Canada (Mss Trombley, Ca"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva","family":"Thomas","sequence":"additional","affiliation":[{"name":"From the Division of Infectious and Immunological Diseases, Department of Pediatrics, University of British Columbia, British Columbia's Research Institute for Child and Family Health, Vancouver, Canada (Drs Pollard and Speert, and Mr Probe); the Clinical Microbiology Laboratory, Department of Pathology, Children's and Women's Health Center of British Columbia, Vancouver, Canada (Mss Trombley, Ca"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"3906","reference":[{"key":"2021012917165401800_i1543-2165-126-10-1209-b1","doi-asserted-by":"crossref","first-page":"1894","DOI":"10.1086\/315158","article-title":"The changing epidemiology of meningococcal disease in the United States, 1992\u20131996.","volume":"180","author":"Rosenstein","year":"1999","journal-title":"J Infect Dis"},{"key":"2021012917165401800_i1543-2165-126-10-1209-b2","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1053\/jinf.1999.0595","article-title":"Clinical features, laboratory findings and management of meningococcal meningitis in England and Wales: report of a 1997 survey. 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