{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T15:31:10Z","timestamp":1770823870967,"version":"3.50.1"},"reference-count":26,"publisher":"Archives of Pathology and Laboratory Medicine","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Context.<\/jats:italic>\n                    \u2014Granulomatous pulmonary nodules are common in areas endemic for\n                    <jats:italic>Histoplasma<\/jats:italic>\n                    infection, and may require surgical excision to exclude neoplasia. Surgeons may elect to routinely send material directly to the clinical microbiology laboratory for fungal and mycobacterial cultures.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Objective.<\/jats:italic>\n                    \u2014To determine if tissue from surgically excised pulmonary granulomatous nodules removed from patients in a geographic area endemic for\n                    <jats:italic>Histoplasma<\/jats:italic>\n                    infection should be routinely submitted for fungal culture.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Design.<\/jats:italic>\n                    \u2014Retrospective review and comparison of surgical pathology histochemical findings and clinical microbiology results of 30 surgical (wedge) lung excisions that demonstrated granulomatous nodule at the time of frozen section.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Results.<\/jats:italic>\n                    \u2014Twenty cases demonstrated fungal organisms consistent with\n                    <jats:italic>Histoplasma<\/jats:italic>\n                    species via histochemical fungal stains. Of these 20 cases, 17 were tested in the microbiology laboratory using direct smear examination and fungal culture;\n                    <jats:italic>Histoplasma<\/jats:italic>\n                    was detected in 1 case (1\/17). Eight cases revealed no organisms by surgical pathology. Of these, 6 were tested in the microbiology laboratory, and all 6 were negative by culture and direct smear (0\/6). The remaining 2 cases demonstrated organisms other than\n                    <jats:italic>Histoplasma<\/jats:italic>\n                    by surgical pathology examination.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Conclusions.<\/jats:italic>\n                    \u2014Surgical pathology examination of granulomatous pulmonary nodules detected\n                    <jats:italic>Histoplasma<\/jats:italic>\n                    organisms with greater sensitivity than culture and direct smear. There were no false-negative surgical pathology diagnoses when compared with microbiological results. These findings suggest that it is not necessary to routinely send material from solitary pulmonary granulomas for fungal culture when the material is removed from immunocompetent patients in a geographic area endemic for histoplasmosis.\n                  <\/jats:p>","DOI":"10.5858\/2007-131-780-cofcvs","type":"journal-article","created":{"date-parts":[[2021,2,11]],"date-time":"2021-02-11T21:04:39Z","timestamp":1613077479000},"page":"780-783","source":"Crossref","is-referenced-by-count":22,"title":["Comparison of Fungal Culture Versus Surgical Pathology Examination in the Detection of Histoplasma in Surgically Excised Pulmonary Granulomas"],"prefix":"10.5858","volume":"131","author":[{"given":"Jamie A.","family":"Weydert","sequence":"first","affiliation":[{"name":"From the Department of Pathology (Drs Weydert and DeYoung) and the Carver College of Medicine Department of Surgery (Dr Van Natta), University of Iowa, Iowa City. Dr Van Natta is now with the Department of Surgery at Harbor-UCLA Medical Center, Los Angeles, Calif"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timothy L.","family":"Van Natta","sequence":"additional","affiliation":[{"name":"From the Department of Pathology (Drs Weydert and DeYoung) and the Carver College of Medicine Department of Surgery (Dr Van Natta), University of Iowa, Iowa City. Dr Van Natta is now with the Department of Surgery at Harbor-UCLA Medical Center, Los Angeles, Calif"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barry R.","family":"DeYoung","sequence":"additional","affiliation":[{"name":"From the Department of Pathology (Drs Weydert and DeYoung) and the Carver College of Medicine Department of Surgery (Dr Van Natta), University of Iowa, Iowa City. Dr Van Natta is now with the Department of Surgery at Harbor-UCLA Medical Center, Los Angeles, Calif"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"3906","reference":[{"key":"i1543-2165-131-5-780-b1","series-title":"Infect Dis Clin North Am","first-page":"1","article-title":"Histoplasmosis.","volume":"17","author":"Wheat, L. J.","year":"2003","unstructured":"Wheat, L. J. and C. A.Kauffman. Histoplasmosis.Infect Dis Clin North Am2003. 17:1\u201319."},{"key":"i1543-2165-131-5-780-b2","series-title":"Clin Chest Med","first-page":"507","article-title":"Fungal pneumonias: the endemic mycoses.","volume":"20","author":"Goldman, M.","year":"1999","unstructured":"Goldman, M., P. C.Johnson, and G. A.Sarosi. Fungal pneumonias: the endemic mycoses.Clin Chest Med1999. 20:507\u2013519."},{"key":"i1543-2165-131-5-780-b3","article-title":"Histoplasma, blastomyces, coccidioides, and other dimorphic fungi causing systemic mycoses.","author":"Walsh, T. J.","unstructured":"Walsh, T. J., T. G.Mitchell, and D. H.Larone. Histoplasma, blastomyces, coccidioides, and other dimorphic fungi causing systemic mycoses.In: Murray PR, Baron EJ, Pfaller MA, Yolken RH, eds. Manual of Clinical Microbiology. 6th ed. Washington, DC: ASM Press; 1995:749\u2013759."},{"key":"i1543-2165-131-5-780-b4","unstructured":"Centers for Disease Control and Prevention.\n          \n          Available at: http:\/\/www.cdc.gov\/ncidod\/dbmd\/diseaseinfo\/histoplasmosis_t.htm. Accessed May 8, 2006."},{"key":"i1543-2165-131-5-780-b5","series-title":"Semin Respir Infect","first-page":"109","article-title":"The epidemiology of histoplasmosis: a review.","volume":"16","author":"Cano, M. V.","year":"2001","unstructured":"Cano, M. V. and R. A.Hajjeh. The epidemiology of histoplasmosis: a review.Semin Respir Infect2001. 16:109\u2013118."},{"key":"i1543-2165-131-5-780-b6","series-title":"Lung Cancer","first-page":"297","article-title":"FDG-PET imaging and the diagnosis of non-small cell lung cancer in a region of high histoplasmosis prevalence.","volume":"36","author":"Croft, D. R.","year":"2002","unstructured":"Croft, D. R., J.Trapp, and K.Kernstine. et al. FDG-PET imaging and the diagnosis of non-small cell lung cancer in a region of high histoplasmosis prevalence.Lung Cancer2002. 36:297\u2013301."},{"key":"i1543-2165-131-5-780-b7","series-title":"Am J Surg Pathol","first-page":"13","article-title":"Solitary necrotizing granulomas of the lung: differentiating features and etiology.","volume":"4","author":"Ulbright, T. M.","year":"1980","unstructured":"Ulbright, T. M. and A. L.Katzenstein. Solitary necrotizing granulomas of the lung: differentiating features and etiology.Am J Surg Pathol1980. 4:13\u201328."},{"key":"i1543-2165-131-5-780-b8","series-title":"Arch Pathol Lab Med","first-page":"1205","article-title":"Diagnosis of histoplasmosis by antigen detection during an outbreak in Indianapolis, Indiana.","volume":"118","author":"Williams, B.","year":"1994","unstructured":"Williams, B., M.Fojtasek, and P.Connolly-Stringfield. et al. Diagnosis of histoplasmosis by antigen detection during an outbreak in Indianapolis, Indiana.Arch Pathol Lab Med1994. 118:1205\u20131208."},{"key":"i1543-2165-131-5-780-b9","series-title":"Am J Clin Pathol","first-page":"367","article-title":"Histoplasmosis in patients with acquired immunodeficiency syndrome: hematologic and bone marrow manifestations.","volume":"93","author":"Kurtin, P. J.","year":"1990","unstructured":"Kurtin, P. J., D. S.McKinsey, and M. R.Gupta. et al. Histoplasmosis in patients with acquired immunodeficiency syndrome: hematologic and bone marrow manifestations.Am J Clin Pathol1990. 93:367\u2013372."},{"key":"i1543-2165-131-5-780-b10","series-title":"Southern Med J","first-page":"692","article-title":"Utility of bone marrow biopsy for rapid diagnosis of febrile illnesses in patients with human immunodeficiency virus infection.","volume":"93","author":"Luther, J. M.","year":"2000","unstructured":"Luther, J. M., D. L.Lakey, and R. S.Larson. et al. Utility of bone marrow biopsy for rapid diagnosis of febrile illnesses in patients with human immunodeficiency virus infection.Southern Med J2000. 93:692\u2013697."},{"key":"i1543-2165-131-5-780-b11","series-title":"Am J Hem","first-page":"100","article-title":"Bone marrow aspiration, biopsy, and culture in the evaluation of HIV-infected patients for invasive mycobacteria and Histoplasma infections.","volume":"67","author":"Akpek, G.","year":"2001","unstructured":"Akpek, G., S. M.Lee, and D. R.Gagnon. et al. Bone marrow aspiration, biopsy, and culture in the evaluation of HIV-infected patients for invasive mycobacteria and Histoplasma infections.Am J Hem2001. 67:100\u2013106."},{"key":"i1543-2165-131-5-780-b12","series-title":"Am J Med","first-page":"923","article-title":"Histoplasmosis in immunosuppressed patients.","volume":"64","author":"Kauffman, C. A.","year":"1978","unstructured":"Kauffman, C. A., K. S.Israel, and J. W.Smith. et al. Histoplasmosis in immunosuppressed patients.Am J Med1978. 64:923\u2013932."},{"key":"i1543-2165-131-5-780-b13","series-title":"Am J Med","first-page":"617","article-title":"Trephine biopsy of the bone marrow in disseminated histoplasmosis.","volume":"67","author":"Davies, S. F.","year":"1979","unstructured":"Davies, S. F., R. W.McKenna, and G. A.Sarosi. Trephine biopsy of the bone marrow in disseminated histoplasmosis.Am J Med1979. 67:617\u2013622."},{"key":"i1543-2165-131-5-780-b1","series-title":"Infect Dis Clin North Am","first-page":"1","article-title":"Histoplasmosis.","volume":"17","author":"Wheat, L. J.","year":"2003","unstructured":"Wheat, L. J. and C. A.Kauffman. Histoplasmosis.Infect Dis Clin North Am2003. 17:1\u201319."},{"key":"i1543-2165-131-5-780-b2","series-title":"Clin Chest Med","first-page":"507","article-title":"Fungal pneumonias: the endemic mycoses.","volume":"20","author":"Goldman, M.","year":"1999","unstructured":"Goldman, M., P. C.Johnson, and G. A.Sarosi. Fungal pneumonias: the endemic mycoses.Clin Chest Med1999. 20:507\u2013519."},{"key":"i1543-2165-131-5-780-b3","article-title":"Histoplasma, blastomyces, coccidioides, and other dimorphic fungi causing systemic mycoses.","author":"Walsh, T. J.","unstructured":"Walsh, T. J., T. G.Mitchell, and D. H.Larone. Histoplasma, blastomyces, coccidioides, and other dimorphic fungi causing systemic mycoses.In: Murray PR, Baron EJ, Pfaller MA, Yolken RH, eds. Manual of Clinical Microbiology. 6th ed. Washington, DC: ASM Press; 1995:749\u2013759."},{"key":"i1543-2165-131-5-780-b4","unstructured":"Centers for Disease Control and Prevention.\n          \n          Available at: http:\/\/www.cdc.gov\/ncidod\/dbmd\/diseaseinfo\/histoplasmosis_t.htm. Accessed May 8, 2006."},{"key":"i1543-2165-131-5-780-b5","series-title":"Semin Respir Infect","first-page":"109","article-title":"The epidemiology of histoplasmosis: a review.","volume":"16","author":"Cano, M. V.","year":"2001","unstructured":"Cano, M. V. and R. A.Hajjeh. The epidemiology of histoplasmosis: a review.Semin Respir Infect2001. 16:109\u2013118."},{"key":"i1543-2165-131-5-780-b6","series-title":"Lung Cancer","first-page":"297","article-title":"FDG-PET imaging and the diagnosis of non-small cell lung cancer in a region of high histoplasmosis prevalence.","volume":"36","author":"Croft, D. R.","year":"2002","unstructured":"Croft, D. R., J.Trapp, and K.Kernstine. et al. FDG-PET imaging and the diagnosis of non-small cell lung cancer in a region of high histoplasmosis prevalence.Lung Cancer2002. 36:297\u2013301."},{"key":"i1543-2165-131-5-780-b7","series-title":"Am J Surg Pathol","first-page":"13","article-title":"Solitary necrotizing granulomas of the lung: differentiating features and etiology.","volume":"4","author":"Ulbright, T. M.","year":"1980","unstructured":"Ulbright, T. M. and A. L.Katzenstein. Solitary necrotizing granulomas of the lung: differentiating features and etiology.Am J Surg Pathol1980. 4:13\u201328."},{"key":"i1543-2165-131-5-780-b8","series-title":"Arch Pathol Lab Med","first-page":"1205","article-title":"Diagnosis of histoplasmosis by antigen detection during an outbreak in Indianapolis, Indiana.","volume":"118","author":"Williams, B.","year":"1994","unstructured":"Williams, B., M.Fojtasek, and P.Connolly-Stringfield. et al. Diagnosis of histoplasmosis by antigen detection during an outbreak in Indianapolis, Indiana.Arch Pathol Lab Med1994. 118:1205\u20131208."},{"key":"i1543-2165-131-5-780-b9","series-title":"Am J Clin Pathol","first-page":"367","article-title":"Histoplasmosis in patients with acquired immunodeficiency syndrome: hematologic and bone marrow manifestations.","volume":"93","author":"Kurtin, P. J.","year":"1990","unstructured":"Kurtin, P. J., D. S.McKinsey, and M. R.Gupta. et al. Histoplasmosis in patients with acquired immunodeficiency syndrome: hematologic and bone marrow manifestations.Am J Clin Pathol1990. 93:367\u2013372."},{"key":"i1543-2165-131-5-780-b10","series-title":"Southern Med J","first-page":"692","article-title":"Utility of bone marrow biopsy for rapid diagnosis of febrile illnesses in patients with human immunodeficiency virus infection.","volume":"93","author":"Luther, J. M.","year":"2000","unstructured":"Luther, J. M., D. L.Lakey, and R. S.Larson. et al. Utility of bone marrow biopsy for rapid diagnosis of febrile illnesses in patients with human immunodeficiency virus infection.Southern Med J2000. 93:692\u2013697."},{"key":"i1543-2165-131-5-780-b11","series-title":"Am J Hem","first-page":"100","article-title":"Bone marrow aspiration, biopsy, and culture in the evaluation of HIV-infected patients for invasive mycobacteria and Histoplasma infections.","volume":"67","author":"Akpek, G.","year":"2001","unstructured":"Akpek, G., S. M.Lee, and D. R.Gagnon. et al. Bone marrow aspiration, biopsy, and culture in the evaluation of HIV-infected patients for invasive mycobacteria and Histoplasma infections.Am J Hem2001. 67:100\u2013106."},{"key":"i1543-2165-131-5-780-b12","series-title":"Am J Med","first-page":"923","article-title":"Histoplasmosis in immunosuppressed patients.","volume":"64","author":"Kauffman, C. A.","year":"1978","unstructured":"Kauffman, C. A., K. S.Israel, and J. W.Smith. et al. Histoplasmosis in immunosuppressed patients.Am J Med1978. 64:923\u2013932."},{"key":"i1543-2165-131-5-780-b13","series-title":"Am J Med","first-page":"617","article-title":"Trephine biopsy of the bone marrow in disseminated histoplasmosis.","volume":"67","author":"Davies, S. F.","year":"1979","unstructured":"Davies, S. F., R. W.McKenna, and G. A.Sarosi. Trephine biopsy of the bone marrow in disseminated histoplasmosis.Am J Med1979. 67:617\u2013622."}],"container-title":["Archives of Pathology &amp; Laboratory Medicine"],"original-title":[],"link":[{"URL":"https:\/\/aplm.kglmeridian.com\/view\/journals\/arpa\/131\/5\/article-p780.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/meridian.allenpress.com\/aplm\/article-pdf\/131\/5\/780\/2762752\/1543-2165(2007)131%255b780_cofcvs%255d2_0_co_2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/aplm.kglmeridian.com\/downloadpdf\/journals\/arpa\/131\/5\/article-p780.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/aplm.kglmeridian.com\/downloadpdf\/journals\/arpa\/131\/5\/article-p780.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T01:01:09Z","timestamp":1770771669000},"score":1,"resource":{"primary":{"URL":"https:\/\/aplm.kglmeridian.com\/view\/journals\/arpa\/131\/5\/article-p780.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,5]]},"references-count":26,"journal-issue":{"issue":"5"},"URL":"https:\/\/doi.org\/10.5858\/2007-131-780-cofcvs","relation":{},"ISSN":["1543-2165","0003-9985"],"issn-type":[{"value":"1543-2165","type":"electronic"},{"value":"0003-9985","type":"print"}],"subject":[],"published":{"date-parts":[[2007,5]]}}}