{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T13:30:08Z","timestamp":1791379808751,"version":"4.3.3"},"reference-count":62,"publisher":"Archives of Pathology and Laboratory Medicine","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,8]]},"abstract":"\n Context<\/jats:italic>\n .\u2014Cervical cancer screening in women younger than 30\u00a0years relies on cervical cytology because of the poor performance of human papillomavirus (HPV) DNA testing in this age group.\n <\/jats:p>\n \n Objectives<\/jats:italic>\n .\u2014To determine the performance of in-cell HPV E6, E7 mRNA quantification (HPV OncoTect) for the detection of high-grade cervical intraepithelial neoplasia in women younger than 30\u00a0years.\n <\/jats:p>\n \n Design<\/jats:italic>\n .\u2014We analyzed 3133 cytology specimens from a screening population of women aged 19\u201375\u00a0years investigate HPV OncoTect as a triage\/secondary screening test for atypical squamous cells of undetermined significance (ASCUS) and low-grade squamous intraepithelial lesion (LSIL) cytology in women younger than 30\u00a0years. Test results were compared to histology in 246 cases.\n <\/jats:p>\n \n Results<\/jats:italic>\n .\u2014The sensitivity of E6, E7 mRNA was 89% for CIN 2+ and 100% for CIN 3+ lesions in women 30\u00a0years and older. In women younger than 30\u00a0years, the sensitivity of E6, E7 mRNA for CIN 2+ lesions was 88% for CIN 2+ and 92% for CIN 3+ lesions. Abnormal cytology (\u2265ASCUS) exhibited a sensitivity of 89% for CIN 2+ and 100% for CIN 3+ in women 30\u00a0years and older and 96% sensitivity for CIN 2+ and 93% sensitivity for CIN 3+ in women younger than 30. The specificity of E6, E7 mRNA was >80% for CIN 2+ and CIN 3+ in both groups of women compared to a specificity of abnormal cytology of <10% for CIN 2+ and CIN 3+ in both groups.\n <\/jats:p>\n \n Conclusions<\/jats:italic>\n .\u2014HPV OncoTect demonstrates a performance that would be effective for ASCUS\/LSIL triage in women including those younger than 30\u00a0years.\n <\/jats:p>","DOI":"10.5858\/arpa.2011-0180-oa","type":"journal-article","created":{"date-parts":[[2012,7,31]],"date-time":"2012-07-31T11:01:31Z","timestamp":1343732491000},"page":"956-960","source":"Crossref","is-referenced-by-count":18,"title":["Intracellular Human Papillomavirus E6, E7 mRNA Quantification Predicts CIN 2+ in Cervical Biopsies Better Than Papanicolaou Screening for Women Regardless of Age"],"prefix":"10.5858","volume":"136","author":[{"given":"Deirdre","family":"Pierry","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerald","family":"Weiss","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Lack","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judy","family":"Fusco","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3906","reference":[{"issue":"6","key":"i1543-2165-136-8-956-b01","series-title":"Curr Oncol Rep","first-page":"533","article-title":"Human papillomavirus testing for primary cervical cancer screening","volume":"10","author":"Runowicz\n CD,","year":"2008","unstructured":"Runowicz\n CD, \n \n Garozzo\n S\n \n . Human papillomavirus testing for primary cervical cancer screening. Curr Oncol Rep. 2008;10(6):533\u2013537."},{"key":"i1543-2165-136-8-956-b02","series-title":"N Engl J Med","first-page":"1579","article-title":"Human papillomavirus DNA versus Papanicolaou screening tests for cervical cancer","volume":"357","author":"Mayrand\n MH,","year":"2007","unstructured":"Mayrand\n MH, \n \n Duarte-Franco\n E, \n \n Rodrigues\n I\n , et al. \n Human papillomavirus DNA versus Papanicolaou screening tests for cervical cancer. N Engl J Med. 2007;357:1579\u20131588."},{"issue":"suppl 1","key":"i1543-2165-136-8-956-b03","series-title":"J Clin Virol","first-page":"S3","article-title":"History of the use of HPV testing in cervical screening and in the management of abnormal cervical screening results","volume":"45","author":"Cox\n JT","year":"2009","unstructured":"Cox\n JT\n \n . History of the use of HPV testing in cervical screening and in the management of abnormal cervical screening results. J Clin Virol. 2009;45(suppl 1):S3\u2013S12."},{"issue":"9292","key":"i1543-2165-136-8-956-b04","series-title":"Lancet.","first-page":"1831","article-title":"Natural history of cervical human papillomavirus infection in young women: a longitudinal cohort study","volume":"357","author":"Woodman\n CB,","year":"2001","unstructured":"Woodman\n CB, \n \n Collins\n S, \n \n Winter\n H\n , et al. \n Natural history of cervical human papillomavirus infection in young women: a longitudinal cohort study. Lancet.2001;357 (9292):1831\u20131836."},{"key":"i1543-2165-136-8-956-b05","series-title":"SEER Cancer Statistics Review 1975\u20132006","author":"Horner\n MJ,","year":"2009","unstructured":"Horner\n MJ, \n \n Ries\n LAG, \n \n Krapcho\n M\n , et al. \n SEER Cancer Statistics Review 1975\u20132006. Bethesda, MD: National Cancer Institute, National Institutes of Health; 2009."},{"key":"i1543-2165-136-8-956-b06","series-title":"Am J Clin Pathol","first-page":"716","article-title":"High-throughput cervical cancer screening using intracellular human papillomavirus E6 and E7 mRNA quantification by flow cytometry","volume":"123","author":"Narimatsu\n R,","year":"2005","unstructured":"Narimatsu\n R, \n \n Patterson\n BK\n \n . High-throughput cervical cancer screening using intracellular human papillomavirus E6 and E7 mRNA quantification by flow cytometry. Am J Clin Pathol. 2005;123:716\u2013723."},{"issue":"1","key":"i1543-2165-136-8-956-b07","series-title":"Gynecol Oncol","first-page":"89","article-title":"Quantification of intracellular HPV E6, E7 mRNA expression increases the specificity and positive predictive value of cervical cancer screening compared to HPV DNA","volume":"120","author":"Coquillard\n G,","year":"2011","unstructured":"Coquillard\n G, \n \n Palao\n B, \n \n Patterson\n BK\n \n . Quantification of intracellular HPV E6, E7 mRNA expression increases the specificity and positive predictive value of cervical cancer screening compared to HPV DNA. Gynecol Oncol. 2011;120(1):89\u201393."},{"issue":"6","key":"i1543-2165-136-8-956-b08","series-title":"Cell","first-page":"1129","article-title":"The E6 oncoprotein encoded by human papillomavirus types 16 and18 promotes the degradation of p53","volume":"63","author":"Scheffner\n M,","year":"1990","unstructured":"Scheffner\n M, \n \n Werness\n BA, \n \n Huibregtse\n JM\n , et al. \n The E6 oncoprotein encoded by human papillomavirus types 16 and18 promotes the degradation of p53. Cell. 1990;63 (6):1129\u20131136."},{"key":"i1543-2165-136-8-956-b09","series-title":"Proc Natl Acad Sci U S A","first-page":"4549","article-title":"Adenovirus E1A, simian virus 40 tumor antigen, and human papillomavirus E7 protein share the capacity to disrupt the interaction between transcription factor E2F and the retinoblastoma gene product","volume":"89","author":"Chellappan\n S,","year":"1992","unstructured":"Chellappan\n S, \n \n Kraus\n VB, \n \n Kroger\n B\n , et al. \n Adenovirus E1A, simian virus 40 tumor antigen, and human papillomavirus E7 protein share the capacity to disrupt the interaction between transcription factor E2F and the retinoblastoma gene product. Proc Natl Acad Sci U S A. 1992;89:4549\u20134553."},{"issue":"4893","key":"i1543-2165-136-8-956-b10","series-title":"Science","first-page":"934","article-title":"The human papilloma virus-16 E7 oncoprotein is able to bind to the retinoblastoma gene product","volume":"243","author":"Dyson\n N,","year":"1989","unstructured":"Dyson\n N, \n \n Howley\n PM, \n \n Munger\n K\n , et al. \n The human papilloma virus-16 E7 oncoprotein is able to bind to the retinoblastoma gene product. Science. 1989;243(4893):934\u2013937."},{"issue":"2","key":"i1543-2165-136-8-956-b11","series-title":"Int J Cancer","first-page":"276","article-title":"Overexpression of p16INK4A as a specific marker for dysplastic and neoplastic epithelial cells of the cervix uteri","volume":"92","author":"Klaes\n R,","year":"2001","unstructured":"Klaes\n R, \n \n Friedrich\n T, \n \n Spitkovsky\n D\n , et al. \n Overexpression of p16INK4A as a specific marker for dysplastic and neoplastic epithelial cells of the cervix uteri. Int J Cancer. 2001;92 (2):276\u2013284."},{"issue":"1","key":"i1543-2165-136-8-956-b12","series-title":"J Med Virol","first-page":"107","article-title":"Detection of high-risk human papillomavirus E6 and E7 oncogene transcripts in cervical scrapes by nested RT-polymerase chain reaction","volume":"74","author":"Sotlar\n K,","year":"2004","unstructured":"Sotlar\n K, \n \n Stubner\n A, \n \n Diemer\n D\n , et al. \n Detection of high-risk human papillomavirus E6 and E7 oncogene transcripts in cervical scrapes by nested RT-polymerase chain reaction. J Med Virol. 2004;74 (1):107\u2013116."},{"issue":"2","key":"i1543-2165-136-8-956-b13","first-page":"251","article-title":"Ubiquitous presence of E6 and E7 transcripts in human papillomavirus-positive cervical carcinomas regardless of its type","volume":"J Med Virol","author":"Nakagawa\n S,","year":"2000","unstructured":"Nakagawa\n S, \n \n Yoshikawa\n H, \n \n Yasugi\n T\n , et al. \n Ubiquitous presence of E6 and E7 transcripts in human papillomavirus-positive cervical carcinomas regardless of its type. J Med Virol. 2000;62 (2):251\u2013258."},{"key":"i1543-2165-136-8-956-b14","series-title":"Arch Pathol Lab Med","first-page":"361","article-title":"Follow-up findings in young females with high-grade squamous intraepithelial lesion Papanicolaou test results","volume":"135","author":"Zhao\n C,","year":"2011","unstructured":"Zhao\n C, \n \n Kalposi-Novak\n P, \n \n Austin\n M\n \n . Follow-up findings in young females with high-grade squamous intraepithelial lesion Papanicolaou test results. Arch Pathol Lab Med. 2011;135:361\u2013364."},{"key":"i1543-2165-136-8-956-b15","series-title":"Obstet Gynecol","first-page":"1409","article-title":"Cervical cytology screening. ACOG Practice Bulletin No.\u00a0109","volume":"114","year":"2009","unstructured":"American College of Obstetricians and Gynecologists. Cervical cytology screening. ACOG Practice Bulletin No.\u00a0109. Obstet Gynecol. 2009;114:1409\u20131420."},{"issue":"4","key":"i1543-2165-136-8-956-b16","first-page":"181","article-title":"The relation between the results of the HPV OncotectR, the cytological alterations, the risk factors and the viral genotype determined by PCR in peri and post menopausic patients. Ginecolog\u00eda y Obstetricia Cl\u00ednica","volume":"9","author":"Silvia\n P,","year":"2008","unstructured":"Silvia\n P, \n \n Gonz\u00e1lez\n SP, \n \n Henr\u00edquez\n A, \n \n Sainz de la Cuesta\n R\n \n . The relation between the results of the HPV OncotectR, the cytological alterations, the risk factors and the viral genotype determined by PCR in peri and post menopausic patients. Ginecolog\u00eda y Obstetricia Cl\u00ednica2008;9(4):181\u2013186."},{"key":"i1543-2165-136-8-956-b17","series-title":"J Clin Virol","article-title":"A flow cytometric method for quantification of HPV E6, E7 mRNA as a novel biomarker for cervical cancer screening","author":"Kottaridi\n C,","year":"In press","unstructured":"Kottaridi\n C, \n \n Tsiodras\n S, \n \n Georgoulakis\n J\n , et al. \n A flow cytometric method for quantification of HPV E6, E7 mRNA as a novel biomarker for cervical cancer screening. J Clin Virol. In press."},{"issue":"6","key":"i1543-2165-136-8-956-b18","series-title":"Cytometry","first-page":"340","article-title":"Determination of liquid-based cervical cytology specimen adequacy using cellular light scatter and flow cytometry","volume":"46","author":"Grundhoefer\n D,","year":"2001","unstructured":"Grundhoefer\n D, \n \n Patterson\n BK\n \n . Determination of liquid-based cervical cytology specimen adequacy using cellular light scatter and flow cytometry. Cytometry. 2001;46(6):340\u2013344."},{"issue":"1","key":"i1543-2165-136-8-956-b19","series-title":"Cytometry B Clin Cytom","first-page":"37","article-title":"Use of flow cytometry as a quality control device for liquid-based cervical cytology specimens","volume":"78","author":"Kottaridi\n C,","year":"2010","unstructured":"Kottaridi\n C, \n \n Georgoulakis\n J, \n \n Kassanos\n D\n , et al. \n Use of flow cytometry as a quality control device for liquid-based cervical cytology specimens. Cytometry B Clin Cytom. 2010;78(1):37\u201340."},{"issue":"2","key":"i1543-2165-136-8-956-b20","series-title":"Cytometry B Clin Cytom","first-page":"133","article-title":"Rapid, high throughput determination of cervical cytology specimen adequacy using a capillary-based cytometer","volume":"74","author":"Polina\n R,","year":"2008","unstructured":"Polina\n R, \n \n Sturgis\n C, \n \n Patterson\n J, \n \n Patterson\n BK\n \n . Rapid, high throughput determination of cervical cytology specimen adequacy using a capillary-based cytometer. Cytometry B Clin Cytom. 2008;74(2):133\u2013136."},{"issue":"3","key":"i1543-2165-136-8-956-b21","series-title":"Gynecol Oncol","first-page":"340","article-title":"Histologic findings from the cervix among older women with abnormal cervical cytology","volume":"88","author":"Massad\n LS,","year":"2003","unstructured":"Massad\n LS, \n \n Behbakht\n K, \n \n Collins\n YC, \n \n Cejtin\n HE\n \n . Histologic findings from the cervix among older women with abnormal cervical cytology. Gynecol Oncol. 2003;88 (3):340\u2013347."},{"issue":"3","key":"i1543-2165-136-8-956-b22","series-title":"Gynecol Oncol.","first-page":"430","article-title":"HPV mRNA tests for the detection of cervical intraepithelial neoplasia: a systematic review","volume":"20","author":"Burger\n EA,","year":"2011","unstructured":"Burger\n EA, \n \n Korner\n H, \n \n Klemp\n M, \n \n Lauvrak\n V, \n \n Kristiansen\n IS\n \n . HPV mRNA tests for the detection of cervical intraepithelial neoplasia: a systematic review. Gynecol Oncol.2011;20 (3):430\u2013438."},{"issue":"1","key":"i1543-2165-136-8-956-b23","series-title":"Virology","first-page":"132","article-title":"Human papillomavirus type 16 (HPV 16) gene expression and DNA replication in cervical neoplasia: analysis by in situ hybridization","volume":"189","author":"Durst\n M,","year":"1992","unstructured":"Durst\n M, \n \n Glitz\n D, \n \n Schneider\n A, \n \n zur Hausen\n HH\n \n . Human papillomavirus type 16 (HPV 16) gene expression and DNA replication in cervical neoplasia: analysis by in situ hybridization. Virology. 1992;189(1):132\u2013140."},{"issue":"10","key":"i1543-2165-136-8-956-b24","series-title":"J Virol","first-page":"4417","article-title":"The E6 and E7 genes of the human papillomavirus type 16 together are necessary and sufficient for transformation of primary human keratinocytes","volume":"63","author":"Munger\n K,","year":"1989","unstructured":"Munger\n K, \n \n Phelps\n WC, \n \n Bubb\n V, \n \n Howley\n PM, \n \n Schlegel\n R\n \n . The E6 and E7 genes of the human papillomavirus type 16 together are necessary and sufficient for transformation of primary human keratinocytes. J Virol. 1989;63(10):4417\u20134421."},{"issue":"11","key":"i1543-2165-136-8-956-b25","series-title":"Cancer Epidemiol Biomarkers Prev","first-page":"3033","article-title":"Comparison of predictors for high-grade cervical intraepithelial neoplasia in women with abnormal smears","volume":"17","author":"Szarewski\n A,","year":"2008","unstructured":"Szarewski\n A, \n \n Ambroisine\n L, \n \n Cadman\n L\n , et al. \n Comparison of predictors for high-grade cervical intraepithelial neoplasia in women with abnormal smears. Cancer Epidemiol Biomarkers Prev. 2008;17(11):3033\u20133042."},{"issue":"1","key":"i1543-2165-136-8-956-b26","series-title":"Cancer Epidemiol Biomarkers Prev","first-page":"110","article-title":"Natural history of human papillomavirus type 16 virus-like particle antibodies in young women","volume":"13","author":"Ho\n GY,","year":"2004","unstructured":"Ho\n GY, \n \n Studentsov\n YY, \n \n Bierman\n R, \n \n Burk\n RD\n \n . Natural history of human papillomavirus type 16 virus-like particle antibodies in young women. Cancer Epidemiol Biomarkers Prev. 2004;13(1):110\u2013116."},{"issue":"17","key":"i1543-2165-136-8-956-b27","series-title":"JAMA","first-page":"2100","article-title":"Treatment for cervical intraepithelial neoplasia and risk of preterm delivery","volume":"291","author":"Sadler\n L,","year":"2004","unstructured":"Sadler\n L, \n \n Saftlas\n A, \n \n Wang\n W, \n \n Exeter\n M, \n \n Whittaker\n J, \n \n McCowan\n L\n \n . Treatment for cervical intraepithelial neoplasia and risk of preterm delivery. JAMA. 2004;291(17):2100\u20132106."},{"issue":"9509","key":"i1543-2165-136-8-956-b28","series-title":"Lancet","first-page":"489","article-title":"Obstetric outcomes after conservative treatment for intraepithelial or early invasive cervical lesions: systematic review and meta-analysis","volume":"367","author":"Kyrgiou\n M,","year":"2006","unstructured":"Kyrgiou\n M, \n \n Koliopoulos\n G, \n \n Martin-Hirsch\n P, \n \n Arbyn\n M, \n \n Prendiville\n W, \n \n Paraskevaidis\n E\n \n . Obstetric outcomes after conservative treatment for intraepithelial or early invasive cervical lesions: systematic review and meta-analysis. Lancet. 2006;367(9509):489\u2013498."},{"key":"i1543-2165-136-8-956-b29","series-title":"HPV E6, E7 mRNA expression in ectocervical cells (HPV OncoTect) predicts disease progression in women with low-grade intraepithelial neoplasia (LSIL)","author":"Alameda\n F,","year":"2008","unstructured":"Alameda\n F, \n \n Palao\n B\n \n . HPV E6, E7 mRNA expression in ectocervical cells (HPV OncoTect) predicts disease progression in women with low-grade intraepithelial neoplasia (LSIL). Nice, France: Presented at Eurogin; 2008."},{"key":"i1543-2165-136-8-956-b30","first-page":"46","article-title":"Baseline cytology, human papillomavirus testing, and risk for cervical neoplasia: a 10-year cohort analysis. J. Natl. Cancer Inst.","volume":"95","author":"Sherman\n ME,","year":"2003","unstructured":"Sherman\n ME, \n \n Lorincz\n AT, \n \n Scott\n DR\n , et al. \n Baseline cytology, human papillomavirus testing, and risk for cervical neoplasia: a 10-year cohort analysis. J. Natl. Cancer Inst.\n 2003;95:46\u201352."},{"key":"i1543-2165-136-8-956-b31","series-title":"Am Fam Physician","article-title":"(November 2003). \u201cThe 2001 Bethesda System Terminology.\u201d","author":"Apgar\n BS,","unstructured":"Apgar\n BS, \n \n Zoschnick\n L, \n \n Wright\n TC\n \n \n (November 2003). \u201cThe 2001 Bethesda System Terminology.\u201d\n Am Fam Physician\n 68(10):1992-8 PMID 1465580."},{"issue":"6","key":"i1543-2165-136-8-956-b01","series-title":"Curr Oncol Rep","first-page":"533","article-title":"Human papillomavirus testing for primary cervical cancer screening","volume":"10","author":"Runowicz\n CD,","year":"2008","unstructured":"Runowicz\n CD, \n \n Garozzo\n S\n \n . Human papillomavirus testing for primary cervical cancer screening. Curr Oncol Rep. 2008;10(6):533\u2013537."},{"key":"i1543-2165-136-8-956-b02","series-title":"N Engl J Med","first-page":"1579","article-title":"Human papillomavirus DNA versus Papanicolaou screening tests for cervical cancer","volume":"357","author":"Mayrand\n MH,","year":"2007","unstructured":"Mayrand\n MH, \n \n Duarte-Franco\n E, \n \n Rodrigues\n I\n , et al. \n Human papillomavirus DNA versus Papanicolaou screening tests for cervical cancer. N Engl J Med. 2007;357:1579\u20131588."},{"issue":"suppl 1","key":"i1543-2165-136-8-956-b03","series-title":"J Clin Virol","first-page":"S3","article-title":"History of the use of HPV testing in cervical screening and in the management of abnormal cervical screening results","volume":"45","author":"Cox\n JT","year":"2009","unstructured":"Cox\n JT\n \n . History of the use of HPV testing in cervical screening and in the management of abnormal cervical screening results. J Clin Virol. 2009;45(suppl 1):S3\u2013S12."},{"issue":"9292","key":"i1543-2165-136-8-956-b04","series-title":"Lancet.","first-page":"1831","article-title":"Natural history of cervical human papillomavirus infection in young women: a longitudinal cohort study","volume":"357","author":"Woodman\n CB,","year":"2001","unstructured":"Woodman\n CB, \n \n Collins\n S, \n \n Winter\n H\n , et al. \n Natural history of cervical human papillomavirus infection in young women: a longitudinal cohort study. Lancet.2001;357 (9292):1831\u20131836."},{"key":"i1543-2165-136-8-956-b05","series-title":"SEER Cancer Statistics Review 1975\u20132006","author":"Horner\n MJ,","year":"2009","unstructured":"Horner\n MJ, \n \n Ries\n LAG, \n \n Krapcho\n M\n , et al. \n SEER Cancer Statistics Review 1975\u20132006. Bethesda, MD: National Cancer Institute, National Institutes of Health; 2009."},{"key":"i1543-2165-136-8-956-b06","series-title":"Am J Clin Pathol","first-page":"716","article-title":"High-throughput cervical cancer screening using intracellular human papillomavirus E6 and E7 mRNA quantification by flow cytometry","volume":"123","author":"Narimatsu\n R,","year":"2005","unstructured":"Narimatsu\n R, \n \n Patterson\n BK\n \n . High-throughput cervical cancer screening using intracellular human papillomavirus E6 and E7 mRNA quantification by flow cytometry. Am J Clin Pathol. 2005;123:716\u2013723."},{"issue":"1","key":"i1543-2165-136-8-956-b07","series-title":"Gynecol Oncol","first-page":"89","article-title":"Quantification of intracellular HPV E6, E7 mRNA expression increases the specificity and positive predictive value of cervical cancer screening compared to HPV DNA","volume":"120","author":"Coquillard\n G,","year":"2011","unstructured":"Coquillard\n G, \n \n Palao\n B, \n \n Patterson\n BK\n \n . Quantification of intracellular HPV E6, E7 mRNA expression increases the specificity and positive predictive value of cervical cancer screening compared to HPV DNA. Gynecol Oncol. 2011;120(1):89\u201393."},{"issue":"6","key":"i1543-2165-136-8-956-b08","series-title":"Cell","first-page":"1129","article-title":"The E6 oncoprotein encoded by human papillomavirus types 16 and18 promotes the degradation of p53","volume":"63","author":"Scheffner\n M,","year":"1990","unstructured":"Scheffner\n M, \n \n Werness\n BA, \n \n Huibregtse\n JM\n , et al. \n The E6 oncoprotein encoded by human papillomavirus types 16 and18 promotes the degradation of p53. Cell. 1990;63 (6):1129\u20131136."},{"key":"i1543-2165-136-8-956-b09","series-title":"Proc Natl Acad Sci U S A","first-page":"4549","article-title":"Adenovirus E1A, simian virus 40 tumor antigen, and human papillomavirus E7 protein share the capacity to disrupt the interaction between transcription factor E2F and the retinoblastoma gene product","volume":"89","author":"Chellappan\n S,","year":"1992","unstructured":"Chellappan\n S, \n \n Kraus\n VB, \n \n Kroger\n B\n , et al. \n Adenovirus E1A, simian virus 40 tumor antigen, and human papillomavirus E7 protein share the capacity to disrupt the interaction between transcription factor E2F and the retinoblastoma gene product. Proc Natl Acad Sci U S A. 1992;89:4549\u20134553."},{"issue":"4893","key":"i1543-2165-136-8-956-b10","series-title":"Science","first-page":"934","article-title":"The human papilloma virus-16 E7 oncoprotein is able to bind to the retinoblastoma gene product","volume":"243","author":"Dyson\n N,","year":"1989","unstructured":"Dyson\n N, \n \n Howley\n PM, \n \n Munger\n K\n , et al. \n The human papilloma virus-16 E7 oncoprotein is able to bind to the retinoblastoma gene product. Science. 1989;243(4893):934\u2013937."},{"issue":"2","key":"i1543-2165-136-8-956-b11","series-title":"Int J Cancer","first-page":"276","article-title":"Overexpression of p16INK4A as a specific marker for dysplastic and neoplastic epithelial cells of the cervix uteri","volume":"92","author":"Klaes\n R,","year":"2001","unstructured":"Klaes\n R, \n \n Friedrich\n T, \n \n Spitkovsky\n D\n , et al. \n Overexpression of p16INK4A as a specific marker for dysplastic and neoplastic epithelial cells of the cervix uteri. Int J Cancer. 2001;92 (2):276\u2013284."},{"issue":"1","key":"i1543-2165-136-8-956-b12","series-title":"J Med Virol","first-page":"107","article-title":"Detection of high-risk human papillomavirus E6 and E7 oncogene transcripts in cervical scrapes by nested RT-polymerase chain reaction","volume":"74","author":"Sotlar\n K,","year":"2004","unstructured":"Sotlar\n K, \n \n Stubner\n A, \n \n Diemer\n D\n , et al. \n Detection of high-risk human papillomavirus E6 and E7 oncogene transcripts in cervical scrapes by nested RT-polymerase chain reaction. J Med Virol. 2004;74 (1):107\u2013116."},{"issue":"2","key":"i1543-2165-136-8-956-b13","first-page":"251","article-title":"Ubiquitous presence of E6 and E7 transcripts in human papillomavirus-positive cervical carcinomas regardless of its type","volume":"J Med Virol","author":"Nakagawa\n S,","year":"2000","unstructured":"Nakagawa\n S, \n \n Yoshikawa\n H, \n \n Yasugi\n T\n , et al. \n Ubiquitous presence of E6 and E7 transcripts in human papillomavirus-positive cervical carcinomas regardless of its type. J Med Virol. 2000;62 (2):251\u2013258."},{"key":"i1543-2165-136-8-956-b14","series-title":"Arch Pathol Lab Med","first-page":"361","article-title":"Follow-up findings in young females with high-grade squamous intraepithelial lesion Papanicolaou test results","volume":"135","author":"Zhao\n C,","year":"2011","unstructured":"Zhao\n C, \n \n Kalposi-Novak\n P, \n \n Austin\n M\n \n . Follow-up findings in young females with high-grade squamous intraepithelial lesion Papanicolaou test results. Arch Pathol Lab Med. 2011;135:361\u2013364."},{"key":"i1543-2165-136-8-956-b15","series-title":"Obstet Gynecol","first-page":"1409","article-title":"Cervical cytology screening. ACOG Practice Bulletin No.\u00a0109","volume":"114","year":"2009","unstructured":"American College of Obstetricians and Gynecologists. Cervical cytology screening. ACOG Practice Bulletin No.\u00a0109. Obstet Gynecol. 2009;114:1409\u20131420."},{"issue":"4","key":"i1543-2165-136-8-956-b16","first-page":"181","article-title":"The relation between the results of the HPV OncotectR, the cytological alterations, the risk factors and the viral genotype determined by PCR in peri and post menopausic patients. Ginecolog\u00eda y Obstetricia Cl\u00ednica","volume":"9","author":"Silvia\n P,","year":"2008","unstructured":"Silvia\n P, \n \n Gonz\u00e1lez\n SP, \n \n Henr\u00edquez\n A, \n \n Sainz de la Cuesta\n R\n \n . The relation between the results of the HPV OncotectR, the cytological alterations, the risk factors and the viral genotype determined by PCR in peri and post menopausic patients. Ginecolog\u00eda y Obstetricia Cl\u00ednica2008;9(4):181\u2013186."},{"key":"i1543-2165-136-8-956-b17","series-title":"J Clin Virol","article-title":"A flow cytometric method for quantification of HPV E6, E7 mRNA as a novel biomarker for cervical cancer screening","author":"Kottaridi\n C,","year":"In press","unstructured":"Kottaridi\n C, \n \n Tsiodras\n S, \n \n Georgoulakis\n J\n , et al. \n A flow cytometric method for quantification of HPV E6, E7 mRNA as a novel biomarker for cervical cancer screening. J Clin Virol. In press."},{"issue":"6","key":"i1543-2165-136-8-956-b18","series-title":"Cytometry","first-page":"340","article-title":"Determination of liquid-based cervical cytology specimen adequacy using cellular light scatter and flow cytometry","volume":"46","author":"Grundhoefer\n D,","year":"2001","unstructured":"Grundhoefer\n D, \n \n Patterson\n BK\n \n . Determination of liquid-based cervical cytology specimen adequacy using cellular light scatter and flow cytometry. Cytometry. 2001;46(6):340\u2013344."},{"issue":"1","key":"i1543-2165-136-8-956-b19","series-title":"Cytometry B Clin Cytom","first-page":"37","article-title":"Use of flow cytometry as a quality control device for liquid-based cervical cytology specimens","volume":"78","author":"Kottaridi\n C,","year":"2010","unstructured":"Kottaridi\n C, \n \n Georgoulakis\n J, \n \n Kassanos\n D\n , et al. \n Use of flow cytometry as a quality control device for liquid-based cervical cytology specimens. Cytometry B Clin Cytom. 2010;78(1):37\u201340."},{"issue":"2","key":"i1543-2165-136-8-956-b20","series-title":"Cytometry B Clin Cytom","first-page":"133","article-title":"Rapid, high throughput determination of cervical cytology specimen adequacy using a capillary-based cytometer","volume":"74","author":"Polina\n R,","year":"2008","unstructured":"Polina\n R, \n \n Sturgis\n C, \n \n Patterson\n J, \n \n Patterson\n BK\n \n . Rapid, high throughput determination of cervical cytology specimen adequacy using a capillary-based cytometer. Cytometry B Clin Cytom. 2008;74(2):133\u2013136."},{"issue":"3","key":"i1543-2165-136-8-956-b21","series-title":"Gynecol Oncol","first-page":"340","article-title":"Histologic findings from the cervix among older women with abnormal cervical cytology","volume":"88","author":"Massad\n LS,","year":"2003","unstructured":"Massad\n LS, \n \n Behbakht\n K, \n \n Collins\n YC, \n \n Cejtin\n HE\n \n . Histologic findings from the cervix among older women with abnormal cervical cytology. Gynecol Oncol. 2003;88 (3):340\u2013347."},{"issue":"3","key":"i1543-2165-136-8-956-b22","series-title":"Gynecol Oncol.","first-page":"430","article-title":"HPV mRNA tests for the detection of cervical intraepithelial neoplasia: a systematic review","volume":"20","author":"Burger\n EA,","year":"2011","unstructured":"Burger\n EA, \n \n Korner\n H, \n \n Klemp\n M, \n \n Lauvrak\n V, \n \n Kristiansen\n IS\n \n . HPV mRNA tests for the detection of cervical intraepithelial neoplasia: a systematic review. Gynecol Oncol.2011;20 (3):430\u2013438."},{"issue":"1","key":"i1543-2165-136-8-956-b23","series-title":"Virology","first-page":"132","article-title":"Human papillomavirus type 16 (HPV 16) gene expression and DNA replication in cervical neoplasia: analysis by in situ hybridization","volume":"189","author":"Durst\n M,","year":"1992","unstructured":"Durst\n M, \n \n Glitz\n D, \n \n Schneider\n A, \n \n zur Hausen\n HH\n \n . Human papillomavirus type 16 (HPV 16) gene expression and DNA replication in cervical neoplasia: analysis by in situ hybridization. Virology. 1992;189(1):132\u2013140."},{"issue":"10","key":"i1543-2165-136-8-956-b24","series-title":"J Virol","first-page":"4417","article-title":"The E6 and E7 genes of the human papillomavirus type 16 together are necessary and sufficient for transformation of primary human keratinocytes","volume":"63","author":"Munger\n K,","year":"1989","unstructured":"Munger\n K, \n \n Phelps\n WC, \n \n Bubb\n V, \n \n Howley\n PM, \n \n Schlegel\n R\n \n . The E6 and E7 genes of the human papillomavirus type 16 together are necessary and sufficient for transformation of primary human keratinocytes. J Virol. 1989;63(10):4417\u20134421."},{"issue":"11","key":"i1543-2165-136-8-956-b25","series-title":"Cancer Epidemiol Biomarkers Prev","first-page":"3033","article-title":"Comparison of predictors for high-grade cervical intraepithelial neoplasia in women with abnormal smears","volume":"17","author":"Szarewski\n A,","year":"2008","unstructured":"Szarewski\n A, \n \n Ambroisine\n L, \n \n Cadman\n L\n , et al. \n Comparison of predictors for high-grade cervical intraepithelial neoplasia in women with abnormal smears. Cancer Epidemiol Biomarkers Prev. 2008;17(11):3033\u20133042."},{"issue":"1","key":"i1543-2165-136-8-956-b26","series-title":"Cancer Epidemiol Biomarkers Prev","first-page":"110","article-title":"Natural history of human papillomavirus type 16 virus-like particle antibodies in young women","volume":"13","author":"Ho\n GY,","year":"2004","unstructured":"Ho\n GY, \n \n Studentsov\n YY, \n \n Bierman\n R, \n \n Burk\n RD\n \n . Natural history of human papillomavirus type 16 virus-like particle antibodies in young women. Cancer Epidemiol Biomarkers Prev. 2004;13(1):110\u2013116."},{"issue":"17","key":"i1543-2165-136-8-956-b27","series-title":"JAMA","first-page":"2100","article-title":"Treatment for cervical intraepithelial neoplasia and risk of preterm delivery","volume":"291","author":"Sadler\n L,","year":"2004","unstructured":"Sadler\n L, \n \n Saftlas\n A, \n \n Wang\n W, \n \n Exeter\n M, \n \n Whittaker\n J, \n \n McCowan\n L\n \n . Treatment for cervical intraepithelial neoplasia and risk of preterm delivery. JAMA. 2004;291(17):2100\u20132106."},{"issue":"9509","key":"i1543-2165-136-8-956-b28","series-title":"Lancet","first-page":"489","article-title":"Obstetric outcomes after conservative treatment for intraepithelial or early invasive cervical lesions: systematic review and meta-analysis","volume":"367","author":"Kyrgiou\n M,","year":"2006","unstructured":"Kyrgiou\n M, \n \n Koliopoulos\n G, \n \n Martin-Hirsch\n P, \n \n Arbyn\n M, \n \n Prendiville\n W, \n \n Paraskevaidis\n E\n \n . Obstetric outcomes after conservative treatment for intraepithelial or early invasive cervical lesions: systematic review and meta-analysis. Lancet. 2006;367(9509):489\u2013498."},{"key":"i1543-2165-136-8-956-b29","series-title":"HPV E6, E7 mRNA expression in ectocervical cells (HPV OncoTect) predicts disease progression in women with low-grade intraepithelial neoplasia (LSIL)","author":"Alameda\n F,","year":"2008","unstructured":"Alameda\n F, \n \n Palao\n B\n \n . HPV E6, E7 mRNA expression in ectocervical cells (HPV OncoTect) predicts disease progression in women with low-grade intraepithelial neoplasia (LSIL). Nice, France: Presented at Eurogin; 2008."},{"key":"i1543-2165-136-8-956-b30","first-page":"46","article-title":"Baseline cytology, human papillomavirus testing, and risk for cervical neoplasia: a 10-year cohort analysis. J. Natl. Cancer Inst.","volume":"95","author":"Sherman\n ME,","year":"2003","unstructured":"Sherman\n ME, \n \n Lorincz\n AT, \n \n Scott\n DR\n , et al. \n Baseline cytology, human papillomavirus testing, and risk for cervical neoplasia: a 10-year cohort analysis. J. Natl. Cancer Inst.\n 2003;95:46\u201352."},{"key":"i1543-2165-136-8-956-b31","series-title":"Am Fam Physician","article-title":"(November 2003). \u201cThe 2001 Bethesda System Terminology.\u201d","author":"Apgar\n BS,","unstructured":"Apgar\n BS, \n \n Zoschnick\n L, \n \n Wright\n TC\n \n \n (November 2003). \u201cThe 2001 Bethesda System Terminology.\u201d\n Am Fam Physician\n 68(10):1992-8 PMID 1465580."}],"container-title":["Archives of Pathology & Laboratory Medicine"],"original-title":[],"link":[{"URL":"https:\/\/aplm.kglmeridian.com\/view\/journals\/arpa\/136\/8\/article-p956.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/meridian.allenpress.com\/aplm\/article-pdf\/136\/8\/956\/2263877\/arpa_2011-0180-oa.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/aplm.kglmeridian.com\/downloadpdf\/journals\/arpa\/136\/8\/article-p956.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/aplm.kglmeridian.com\/downloadpdf\/journals\/arpa\/136\/8\/article-p956.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T01:14:07Z","timestamp":1770772447000},"score":1,"resource":{"primary":{"URL":"https:\/\/aplm.kglmeridian.com\/view\/journals\/arpa\/136\/8\/article-p956.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,8]]},"references-count":62,"journal-issue":{"issue":"8"},"URL":"https:\/\/doi.org\/10.5858\/arpa.2011-0180-oa","relation":{},"ISSN":["1543-2165","0003-9985"],"issn-type":[{"value":"1543-2165","type":"electronic"},{"value":"0003-9985","type":"print"}],"subject":[],"published":{"date-parts":[[2012,8]]}}}