{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T04:53:00Z","timestamp":1770785580995,"version":"3.50.0"},"reference-count":54,"publisher":"Archives of Pathology and Laboratory Medicine","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,1]]},"abstract":"\n Context<\/jats:italic>\n .\u2014The detection and typing of high-risk and low-risk human papillomavirus (HPV) in archival formalin-fixed, paraffin-embedded tissues by nucleic acid amplification testing is an important adjunct to immunohistochemical staining in evaluation of squamous cell proliferations of the oropharynx, larynx, and anal canal.\n <\/jats:p>\n \n Objective<\/jats:italic>\n .\u2014To evaluate semiautomated, xylene-free extraction from formalin-fixed, paraffin-embedded tissues combined with laboratory-developed HPV L1 sequencing and type-specific HPV 6, 11, 16, and 18 real-time polymerase chain reaction for identification and typing of HPV in the clinical laboratory.\n <\/jats:p>\n \n Design<\/jats:italic>\n .\u2014We evaluated the adequacy of extraction using \u03b2-globin amplification and compared L1 sequencing and real-time polymerase chain reaction methods for typing accuracy using 68 formalin-fixed, paraffin-embedded tissues, including 56 anorectal biopsy or surgical resection specimens and 12 laryngeal papilloma specimens from patients with recurrent respiratory papillomatosis.\n <\/jats:p>\n \n Results<\/jats:italic>\n .\u2014Adequate DNA was obtained from 68 of 68 specimens analyzed and all were HPV positive. In 47 cases where L1 sequencing demonstrated that the predominant HPV type was 6, 11, 16, or 18, type-specific, real-time polymerase chain reaction provided concordant results. Sequencing revealed additional low-risk (HPV 40) and high-risk HPV types (HPV 31, 33, 56, and 58) in anorectal specimens, whereas HPV 6 or 11 were the types found in laryngeal papillomas.\n <\/jats:p>\n \n Conclusion<\/jats:italic>\n .\u2014Both L1 sequencing and type-specific, real-time polymerase chain reaction are suitable methods for routine HPV testing of formalin-fixed, paraffin-embedded tissues in a clinical laboratory setting.\n <\/jats:p>","DOI":"10.5858\/arpa.2011-0392-oa","type":"journal-article","created":{"date-parts":[[2012,12,31]],"date-time":"2012-12-31T14:51:17Z","timestamp":1356965477000},"page":"50-54","source":"Crossref","is-referenced-by-count":8,"title":["Laboratory-Developed L1 Sequencing and Type-Specific, Real-Time Polymerase Chain Reaction for the Detection and Typing of Human Papillomaviruses in Formalin-Fixed, Paraffin-Embedded Tissues"],"prefix":"10.5858","volume":"137","author":[{"given":"Anne","family":"Mills","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rajeev","family":"Balasubramaniam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teri A.","family":"Longacre","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christina S.","family":"Kong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benjamin A.","family":"Pinsky","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"3906","reference":[{"issue":"9","key":"i1543-2165-137-1-50-b01","series-title":"Laryngoscope","first-page":"1756","article-title":"Human papillomavirus and oropharynx cancer: biology, detection and clinical implications","volume":"120","author":"Allen\n CT ,","year":"2010;","unstructured":"Allen\n CT , \n \n Lewis\n JS\n Jr , \n \n El-Mofty\n SK , \n \n Haughey\n BH , \n \n Nussenbaum\n B . \n \n Human papillomavirus and oropharynx cancer: biology, detection and clinical implications . Laryngoscope . 2010;120(9) :1756 \u20131772 ."},{"issue":"6","key":"i1543-2165-137-1-50-b02","series-title":"Oral Oncol","first-page":"486","article-title":"Oropharyngeal carcinoma in non-smokers and non-drinkers: a role for HPV","volume":"45","author":"Andrews\n E ,","year":"2009","unstructured":"Andrews\n E , \n \n Seaman\n WT , \n \n Webster-Cyriaque\n J . \n \n Oropharyngeal carcinoma in non-smokers and non-drinkers: a role for HPV . Oral Oncol . 2009 ;45(6) :486 \u2013491 ."},{"issue":"4","key":"i1543-2165-137-1-50-b03","series-title":"Am J Otolaryngol","first-page":"291","article-title":"Malignant transformation of a highly aggressive human papillomavirus type 11-associated recurrent respiratory papillomatosis","volume":"31","author":"Lin\n HW ,","year":"2010;","unstructured":"Lin\n HW , \n \n Richmon\n JD , \n \n Emerick\n KS , \n et al\n . Malignant transformation of a highly aggressive human papillomavirus type 11-associated recurrent respiratory papillomatosis . Am J Otolaryngol . 2010;31(4) :291 \u2013296 ."},{"issue":"7","key":"i1543-2165-137-1-50-b04","series-title":"Laryngoscope","first-page":"915","article-title":"Pediatric respiratory papillomatosis: prognostic role of viral typing and cofactors","volume":"107","author":"Rimell\n FL ,","year":"1997","unstructured":"Rimell\n FL , \n \n Shoemaker\n DL , \n \n Pou\n AM , \n \n Jordan\n JA , \n \n Post\n JC , \n \n Ehrlich\n GD . \n \n Pediatric respiratory papillomatosis: prognostic role of viral typing and cofactors . Laryngoscope . 1997 ;107(7) :915 \u2013918 ."},{"issue":"6","key":"i1543-2165-137-1-50-b05","series-title":"PLoS One","article-title":"The isolation of nucleic acids from fixed, paraffin-embedded tissues\u2014which methods are useful when?","volume":"2","author":"Gilbert\n MT ,","year":"2007;","unstructured":"Gilbert\n MT , \n \n Haselkorn\n T , \n \n Bunce\n M , \n et al\n . The isolation of nucleic acids from fixed, paraffin-embedded tissues\u2014which methods are useful when?PLoS One . 2007;2(6) :e537 ."},{"issue":"6","key":"i1543-2165-137-1-50-b06","series-title":"Am J Surg Pathol","first-page":"919","article-title":"Role of human papillomavirus in squamous cell metaplasia-dysplasia-carcinoma of the rectum","volume":"31","author":"Kong\n CS ,","year":"2007","unstructured":"Kong\n CS , \n \n Welton\n ML , \n \n Longacre\n TA . \n \n Role of human papillomavirus in squamous cell metaplasia-dysplasia-carcinoma of the rectum . Am J Surg Pathol . 2007 ;31(6) :919 \u2013925 ."},{"issue":"1","key":"i1543-2165-137-1-50-b07","series-title":"N Engl J Med","first-page":"24","article-title":"Human papillomavirus and survival of patients with oropharyngeal cancer","volume":"363","author":"Ang\n KK ,","year":"2010;","unstructured":"Ang\n KK , \n \n Harris\n J , \n \n Wheeler\n R , \n et al\n . Human papillomavirus and survival of patients with oropharyngeal cancer . N Engl J Med . 2010;363(1) :24 \u201335 ."},{"issue":"7","key":"i1543-2165-137-1-50-b08","series-title":"Am J Surg Pathol","first-page":"915","article-title":"A panel of 3 markers including p16, ProExC, or HPV ISH is optimal for distinguishing between primary endometrial and endocervical adenocarcinomas","volume":"34","author":"Kong\n CS ,","year":"2010","unstructured":"Kong\n CS , \n \n Beck\n AH , \n \n Longacre\n TA . \n \n A panel of 3 markers including p16, ProExC, or HPV ISH is optimal for distinguishing between primary endometrial and endocervical adenocarcinomas . Am J Surg Pathol . 2010 ;34(7) :915 \u2013926 ."},{"issue":"2","key":"i1543-2165-137-1-50-b09","series-title":"Cancer","first-page":"118","article-title":"Detection of human papillomavirus-related squamous cell carcinoma cytologically and by in situ hybridization in fine-needle aspiration biopsies of cervical metastasis: a tool for identifying the site of an occult head and neck primary","volume":"114","author":"Zhang\n MQ ,","year":"2008","unstructured":"Zhang\n MQ , \n \n El-Mofty\n SK , \n \n Davila\n RM . \n \n Detection of human papillomavirus-related squamous cell carcinoma cytologically and by in situ hybridization in fine-needle aspiration biopsies of cervical metastasis: a tool for identifying the site of an occult head and neck primary . Cancer . 2008 ;114(2) :118 \u2013123 ."},{"issue":"6","key":"i1543-2165-137-1-50-b10","series-title":"Am J Surg Pathol","first-page":"899","article-title":"ProEx C: a sensitive and specific marker of HPV-associated squamous lesions of the cervix","volume":"32","author":"Badr\n RE ,","year":"2008","unstructured":"Badr\n RE , \n \n Walts\n AE , \n \n Chung\n F , \n \n Bose\n S. BD\n \n \n ProEx C: a sensitive and specific marker of HPV-associated squamous lesions of the cervix . Am J Surg Pathol . 2008 ;32(6) :899 \u2013906 ."},{"issue":"8","key":"i1543-2165-137-1-50-b11","series-title":"Int J Cancer","first-page":"1731","article-title":"Combined analysis of HPV-DNA, p16 and EGFR expression to predict prognosis in oropharyngeal cancer","volume":"120","author":"Reimers\n N ,","year":"2007;","unstructured":"Reimers\n N , \n \n Kasper\n HU , \n \n Weissenborn\n SJ , \n et al\n . Combined analysis of HPV-DNA, p16 and EGFR expression to predict prognosis in oropharyngeal cancer . Int J Cancer . 2007;120(8) :1731 \u20131738 ."},{"issue":"36","key":"i1543-2165-137-1-50-b12","series-title":"J Clin Oncol","first-page":"6213","article-title":"Comparative prognostic value of HPV16 E6 mRNA compared with in situ hybridization for human oropharyngeal squamous carcinoma","volume":"27","author":"Shi\n W ,","year":"2009;","unstructured":"Shi\n W , \n \n Kato\n H , \n \n Perez-Ordonez\n B , \n et al\n . Comparative prognostic value of HPV16 E6 mRNA compared with in situ hybridization for human oropharyngeal squamous carcinoma . J Clin Oncol . 2009;27(36) :6213 \u20136221 ."},{"issue":"11","key":"i1543-2165-137-1-50-b13","series-title":"Int J Cancer","first-page":"2465","article-title":"A novel algorithm for reliable detection of human papillomavirus in paraffin embedded head and neck cancer specimen","volume":"121","author":"Smeets\n SJ ,","year":"2007;","unstructured":"Smeets\n SJ , \n \n Hesselink\n AT , \n \n Speel\n EJ , \n et al\n . A novel algorithm for reliable detection of human papillomavirus in paraffin embedded head and neck cancer specimen . Int J Cancer . 2007;121(11) :2465 \u20132472 ."},{"issue":"10","key":"i1543-2165-137-1-50-b14","series-title":"Oncogene","first-page":"1510","article-title":"Involvement of intact HPV16 E6\/E7 gene expression in head and neck cancers with unaltered p53 status and perturbed pRb cell cycle control","volume":"21","author":"Wiest\n T ,","year":"2002","unstructured":"Wiest\n T , \n \n Schwarz\n E , \n \n Enders\n C , \n \n Flechtenmacher\n C , \n \n Bosch\n FX . \n \n Involvement of intact HPV16 E6\/E7 gene expression in head and neck cancers with unaltered p53 status and perturbed pRb cell cycle control . Oncogene . 2002 ;21(10) :1510 \u20131517 ."},{"issue":"4","key":"i1543-2165-137-1-50-b15","series-title":"J Clin Virol","first-page":"302","article-title":"Quantitative multiplex PCR assay for the detection of the seven clinically most relevant high-risk HPV types","volume":"44","author":"Schmitz\n M ,","year":"2009;","unstructured":"Schmitz\n M , \n \n Scheungraber\n C , \n \n Herrmann\n J , \n et al\n . Quantitative multiplex PCR assay for the detection of the seven clinically most relevant high-risk HPV types . J Clin Virol . 2009;44(4) :302 \u2013307 ."},{"issue":"8","key":"i1543-2165-137-1-50-b16","series-title":"Eur J Pediatr","first-page":"1017","article-title":"Novel human papilloma virus (HPV) genotypes in children with recurrent respiratory papillomatosis","volume":"169","author":"Mammas\n IN ,","year":"2010","unstructured":"Mammas\n IN , \n \n Sourvinos\n G , \n \n Vakonaki\n E , \n \n Giamarelou\n P , \n \n Michael\n C , \n \n Spandidos\n DA . \n \n Novel human papilloma virus (HPV) genotypes in children with recurrent respiratory papillomatosis . Eur J Pediatr . 2010 ;169(8) :1017 \u20131021 ."},{"issue":"3","key":"i1543-2165-137-1-50-b17","series-title":"J Clin Microbiol","first-page":"541","article-title":"Modified general primer PCR system for sensitive detection of multiple types of oncogenic human papillomavirus","volume":"47","author":"Soderlund-Strand\n A ,","year":"2009","unstructured":"Soderlund-Strand\n A , \n \n Carlson\n J , \n \n Dillner\n J . \n \n Modified general primer PCR system for sensitive detection of multiple types of oncogenic human papillomavirus . J Clin Microbiol . 2009 ;47(3) :541 \u2013546 ."},{"issue":"10","key":"i1543-2165-137-1-50-b18","series-title":"Expert Rev Anti Infect Ther","first-page":"1139","article-title":"Commercially available assays for multiplex detection of alpha human papillomaviruses","volume":"8","author":"Poljak\n M ,","year":"2010","unstructured":"Poljak\n M , \n \n Kocjan\n BJ . \n \n Commercially available assays for multiplex detection of alpha human papillomaviruses . Expert Rev Anti Infect Ther . 2010 ;8(10) :1139 \u20131162 ."},{"issue":"4","key":"i1543-2165-137-1-50-b19","series-title":"J Mol Diagn","first-page":"377","article-title":"Efficient DNA extraction for HPV genotyping in formalin-fixed, paraffin-embedded tissues","volume":"13","author":"Steinau\n M ,","year":"2011","unstructured":"Steinau\n M , \n \n Patel\n SS , \n \n Unger\n ER . \n \n Efficient DNA extraction for HPV genotyping in formalin-fixed, paraffin-embedded tissues . J Mol Diagn . 2011 ;13(4) :377 \u2013381 ."},{"issue":"12","key":"i1543-2165-137-1-50-b20","series-title":"Arch Pathol Lab Med","first-page":"1813","article-title":"Use of a linear array for the detection of human papillomavirus genotypes in head and neck cancer","volume":"134","author":"Black\n CC ,","year":"2010","unstructured":"Black\n CC , \n \n Bentley\n HA , \n \n Davis\n TH , \n \n Tsongalis\n GJ . \n \n Use of a linear array for the detection of human papillomavirus genotypes in head and neck cancer . Arch Pathol Lab Med . 2010 ;134(12) :1813 \u20131817 ."},{"issue":"1","key":"i1543-2165-137-1-50-b21","series-title":"J Virol Methods","first-page":"117","article-title":"Comparison of the Abbott RealTime High Risk HPV test and INNO-LiPA HPV Genotyping Extra test for the detection of human papillomaviruses in formalin-fixed, paraffin-embedded cervical cancer specimens","volume":"175","author":"Kocjan\n BJ ,","year":"2011","unstructured":"Kocjan\n BJ , \n \n Seme\n K , \n \n Poljak\n M . \n \n Comparison of the Abbott RealTime High Risk HPV test and INNO-LiPA HPV Genotyping Extra test for the detection of human papillomaviruses in formalin-fixed, paraffin-embedded cervical cancer specimens . J Virol Methods . 2011 ;175(1) :117 \u2013119 ."},{"issue":"4","key":"i1543-2165-137-1-50-b22","series-title":"J Clin Microbiol","first-page":"1458","article-title":"Human papillomavirus genotyping using archival vulval dysplastic or neoplastic biopsy tissues: comparison between the INNO-LiPA and linear array assays","volume":"48","author":"Tan\n SE ,","year":"2010","unstructured":"Tan\n SE , \n \n Garland\n SM , \n \n Rumbold\n AR , \n \n Tabrizi\n SN . \n \n Human papillomavirus genotyping using archival vulval dysplastic or neoplastic biopsy tissues: comparison between the INNO-LiPA and linear array assays . J Clin Microbiol . 2010 ;48(4) :1458 \u20131460 ."},{"key":"i1543-2165-137-1-50-b23","series-title":"Infect Agent Cancer","first-page":"2","article-title":"Rapid, sensitive, type specific PCR detection of the E7 region of human papillomavirus type 16 and 18 from paraffin embedded sections of cervical carcinoma","volume":"5","author":"Lesnikova\n I ,","year":"2010","unstructured":"Lesnikova\n I , \n \n Lidang\n M , \n \n Hamilton-Dutoit\n S , \n \n Koch\n J . \n \n Rapid, sensitive, type specific PCR detection of the E7 region of human papillomavirus type 16 and 18 from paraffin embedded sections of cervical carcinoma . Infect Agent Cancer . 2010 ;5 :2 ."},{"issue":"3","key":"i1543-2165-137-1-50-b24","series-title":"Gynecol Oncol","first-page":"555","article-title":"HPV genotyping in cervical cancer in Northern Thailand: adapting the linear array HPV assay for use on paraffin-embedded tissue","volume":"108","author":"Siriaunkgul\n S ,","year":"2008;","unstructured":"Siriaunkgul\n S , \n \n Suwiwat\n S , \n \n Settakorn\n J , \n et al\n . HPV genotyping in cervical cancer in Northern Thailand: adapting the linear array HPV assay for use on paraffin-embedded tissue . Gynecol Oncol . 2008;108(3) :555 \u2013560 ."},{"issue":"8","key":"i1543-2165-137-1-50-b25","series-title":"J Clin Microbiol","first-page":"3758","article-title":"Comparison of real-time PCR signal-amplified in situ hybridization and conventional PCR for detection and quantification of human papillomavirus in archival cervical cancer tissue","volume":"42","author":"Biedermann\n K ,","year":"2004;","unstructured":"Biedermann\n K , \n \n Dandachi\n N , \n \n Trattner\n M , \n et al\n . Comparison of real-time PCR signal-amplified in situ hybridization and conventional PCR for detection and quantification of human papillomavirus in archival cervical cancer tissue . J Clin Microbiol . 2004;42(8) :3758 \u20133765 ."},{"issue":"10","key":"i1543-2165-137-1-50-b26","series-title":"Am J Surg Pathol","first-page":"1449","article-title":"P16\/CDKN2A and Ki-67 enhance the detection of anal intraepithelial neoplasia and condyloma and correlate with human papillomavirus detection by polymerase chain reaction","volume":"34","author":"Pirog\n EC ,","year":"2010","unstructured":"Pirog\n EC , \n \n Quint\n KD , \n \n Yantiss\n RK . \n \n P16\/CDKN2A and Ki-67 enhance the detection of anal intraepithelial neoplasia and condyloma and correlate with human papillomavirus detection by polymerase chain reaction . Am J Surg Pathol . 2010 ;34(10) :1449 \u20131455 ."},{"issue":"14","key":"i1543-2165-137-1-50-b27","series-title":"J Natl Cancer Inst","first-page":"1072","article-title":"The elevated 10-year risk of cervical precancer and cancer in women with human papillomavirus (HPV) type 16 or 18 and the possible utility of type-specific HPV testing in clinical practice","volume":"97","author":"Khan\n MJ ,","year":"2005;","unstructured":"Khan\n MJ , \n \n Castle\n PE , \n \n Lorincz\n AT , \n et al\n . The elevated 10-year risk of cervical precancer and cancer in women with human papillomavirus (HPV) type 16 or 18 and the possible utility of type-specific HPV testing in clinical practice . J Natl Cancer Inst . 2005;97(14) :1072 \u20131079 ."},{"issue":"9","key":"i1543-2165-137-1-50-b01","series-title":"Laryngoscope","first-page":"1756","article-title":"Human papillomavirus and oropharynx cancer: biology, detection and clinical implications","volume":"120","author":"Allen\n CT ,","year":"2010;","unstructured":"Allen\n CT , \n \n Lewis\n JS\n Jr , \n \n El-Mofty\n SK , \n \n Haughey\n BH , \n \n Nussenbaum\n B . \n \n Human papillomavirus and oropharynx cancer: biology, detection and clinical implications . Laryngoscope . 2010;120(9) :1756 \u20131772 ."},{"issue":"6","key":"i1543-2165-137-1-50-b02","series-title":"Oral Oncol","first-page":"486","article-title":"Oropharyngeal carcinoma in non-smokers and non-drinkers: a role for HPV","volume":"45","author":"Andrews\n E ,","year":"2009","unstructured":"Andrews\n E , \n \n Seaman\n WT , \n \n Webster-Cyriaque\n J . \n \n Oropharyngeal carcinoma in non-smokers and non-drinkers: a role for HPV . Oral Oncol . 2009 ;45(6) :486 \u2013491 ."},{"issue":"4","key":"i1543-2165-137-1-50-b03","series-title":"Am J Otolaryngol","first-page":"291","article-title":"Malignant transformation of a highly aggressive human papillomavirus type 11-associated recurrent respiratory papillomatosis","volume":"31","author":"Lin\n HW ,","year":"2010;","unstructured":"Lin\n HW , \n \n Richmon\n JD , \n \n Emerick\n KS , \n et al\n . Malignant transformation of a highly aggressive human papillomavirus type 11-associated recurrent respiratory papillomatosis . Am J Otolaryngol . 2010;31(4) :291 \u2013296 ."},{"issue":"7","key":"i1543-2165-137-1-50-b04","series-title":"Laryngoscope","first-page":"915","article-title":"Pediatric respiratory papillomatosis: prognostic role of viral typing and cofactors","volume":"107","author":"Rimell\n FL ,","year":"1997","unstructured":"Rimell\n FL , \n \n Shoemaker\n DL , \n \n Pou\n AM , \n \n Jordan\n JA , \n \n Post\n JC , \n \n Ehrlich\n GD . \n \n Pediatric respiratory papillomatosis: prognostic role of viral typing and cofactors . Laryngoscope . 1997 ;107(7) :915 \u2013918 ."},{"issue":"6","key":"i1543-2165-137-1-50-b05","series-title":"PLoS One","article-title":"The isolation of nucleic acids from fixed, paraffin-embedded tissues\u2014which methods are useful when?","volume":"2","author":"Gilbert\n MT ,","year":"2007;","unstructured":"Gilbert\n MT , \n \n Haselkorn\n T , \n \n Bunce\n M , \n et al\n . The isolation of nucleic acids from fixed, paraffin-embedded tissues\u2014which methods are useful when?PLoS One . 2007;2(6) :e537 ."},{"issue":"6","key":"i1543-2165-137-1-50-b06","series-title":"Am J Surg Pathol","first-page":"919","article-title":"Role of human papillomavirus in squamous cell metaplasia-dysplasia-carcinoma of the rectum","volume":"31","author":"Kong\n CS ,","year":"2007","unstructured":"Kong\n CS , \n \n Welton\n ML , \n \n Longacre\n TA . \n \n Role of human papillomavirus in squamous cell metaplasia-dysplasia-carcinoma of the rectum . Am J Surg Pathol . 2007 ;31(6) :919 \u2013925 ."},{"issue":"1","key":"i1543-2165-137-1-50-b07","series-title":"N Engl J Med","first-page":"24","article-title":"Human papillomavirus and survival of patients with oropharyngeal cancer","volume":"363","author":"Ang\n KK ,","year":"2010;","unstructured":"Ang\n KK , \n \n Harris\n J , \n \n Wheeler\n R , \n et al\n . Human papillomavirus and survival of patients with oropharyngeal cancer . N Engl J Med . 2010;363(1) :24 \u201335 ."},{"issue":"7","key":"i1543-2165-137-1-50-b08","series-title":"Am J Surg Pathol","first-page":"915","article-title":"A panel of 3 markers including p16, ProExC, or HPV ISH is optimal for distinguishing between primary endometrial and endocervical adenocarcinomas","volume":"34","author":"Kong\n CS ,","year":"2010","unstructured":"Kong\n CS , \n \n Beck\n AH , \n \n Longacre\n TA . \n \n A panel of 3 markers including p16, ProExC, or HPV ISH is optimal for distinguishing between primary endometrial and endocervical adenocarcinomas . Am J Surg Pathol . 2010 ;34(7) :915 \u2013926 ."},{"issue":"2","key":"i1543-2165-137-1-50-b09","series-title":"Cancer","first-page":"118","article-title":"Detection of human papillomavirus-related squamous cell carcinoma cytologically and by in situ hybridization in fine-needle aspiration biopsies of cervical metastasis: a tool for identifying the site of an occult head and neck primary","volume":"114","author":"Zhang\n MQ ,","year":"2008","unstructured":"Zhang\n MQ , \n \n El-Mofty\n SK , \n \n Davila\n RM . \n \n Detection of human papillomavirus-related squamous cell carcinoma cytologically and by in situ hybridization in fine-needle aspiration biopsies of cervical metastasis: a tool for identifying the site of an occult head and neck primary . Cancer . 2008 ;114(2) :118 \u2013123 ."},{"issue":"6","key":"i1543-2165-137-1-50-b10","series-title":"Am J Surg Pathol","first-page":"899","article-title":"ProEx C: a sensitive and specific marker of HPV-associated squamous lesions of the cervix","volume":"32","author":"Badr\n RE ,","year":"2008","unstructured":"Badr\n RE , \n \n Walts\n AE , \n \n Chung\n F , \n \n Bose\n S. BD\n \n \n ProEx C: a sensitive and specific marker of HPV-associated squamous lesions of the cervix . Am J Surg Pathol . 2008 ;32(6) :899 \u2013906 ."},{"issue":"8","key":"i1543-2165-137-1-50-b11","series-title":"Int J Cancer","first-page":"1731","article-title":"Combined analysis of HPV-DNA, p16 and EGFR expression to predict prognosis in oropharyngeal cancer","volume":"120","author":"Reimers\n N ,","year":"2007;","unstructured":"Reimers\n N , \n \n Kasper\n HU , \n \n Weissenborn\n SJ , \n et al\n . Combined analysis of HPV-DNA, p16 and EGFR expression to predict prognosis in oropharyngeal cancer . Int J Cancer . 2007;120(8) :1731 \u20131738 ."},{"issue":"36","key":"i1543-2165-137-1-50-b12","series-title":"J Clin Oncol","first-page":"6213","article-title":"Comparative prognostic value of HPV16 E6 mRNA compared with in situ hybridization for human oropharyngeal squamous carcinoma","volume":"27","author":"Shi\n W ,","year":"2009;","unstructured":"Shi\n W , \n \n Kato\n H , \n \n Perez-Ordonez\n B , \n et al\n . Comparative prognostic value of HPV16 E6 mRNA compared with in situ hybridization for human oropharyngeal squamous carcinoma . J Clin Oncol . 2009;27(36) :6213 \u20136221 ."},{"issue":"11","key":"i1543-2165-137-1-50-b13","series-title":"Int J Cancer","first-page":"2465","article-title":"A novel algorithm for reliable detection of human papillomavirus in paraffin embedded head and neck cancer specimen","volume":"121","author":"Smeets\n SJ ,","year":"2007;","unstructured":"Smeets\n SJ , \n \n Hesselink\n AT , \n \n Speel\n EJ , \n et al\n . A novel algorithm for reliable detection of human papillomavirus in paraffin embedded head and neck cancer specimen . Int J Cancer . 2007;121(11) :2465 \u20132472 ."},{"issue":"10","key":"i1543-2165-137-1-50-b14","series-title":"Oncogene","first-page":"1510","article-title":"Involvement of intact HPV16 E6\/E7 gene expression in head and neck cancers with unaltered p53 status and perturbed pRb cell cycle control","volume":"21","author":"Wiest\n T ,","year":"2002","unstructured":"Wiest\n T , \n \n Schwarz\n E , \n \n Enders\n C , \n \n Flechtenmacher\n C , \n \n Bosch\n FX . \n \n Involvement of intact HPV16 E6\/E7 gene expression in head and neck cancers with unaltered p53 status and perturbed pRb cell cycle control . Oncogene . 2002 ;21(10) :1510 \u20131517 ."},{"issue":"4","key":"i1543-2165-137-1-50-b15","series-title":"J Clin Virol","first-page":"302","article-title":"Quantitative multiplex PCR assay for the detection of the seven clinically most relevant high-risk HPV types","volume":"44","author":"Schmitz\n M ,","year":"2009;","unstructured":"Schmitz\n M , \n \n Scheungraber\n C , \n \n Herrmann\n J , \n et al\n . Quantitative multiplex PCR assay for the detection of the seven clinically most relevant high-risk HPV types . J Clin Virol . 2009;44(4) :302 \u2013307 ."},{"issue":"8","key":"i1543-2165-137-1-50-b16","series-title":"Eur J Pediatr","first-page":"1017","article-title":"Novel human papilloma virus (HPV) genotypes in children with recurrent respiratory papillomatosis","volume":"169","author":"Mammas\n IN ,","year":"2010","unstructured":"Mammas\n IN , \n \n Sourvinos\n G , \n \n Vakonaki\n E , \n \n Giamarelou\n P , \n \n Michael\n C , \n \n Spandidos\n DA . \n \n Novel human papilloma virus (HPV) genotypes in children with recurrent respiratory papillomatosis . Eur J Pediatr . 2010 ;169(8) :1017 \u20131021 ."},{"issue":"3","key":"i1543-2165-137-1-50-b17","series-title":"J Clin Microbiol","first-page":"541","article-title":"Modified general primer PCR system for sensitive detection of multiple types of oncogenic human papillomavirus","volume":"47","author":"Soderlund-Strand\n A ,","year":"2009","unstructured":"Soderlund-Strand\n A , \n \n Carlson\n J , \n \n Dillner\n J . \n \n Modified general primer PCR system for sensitive detection of multiple types of oncogenic human papillomavirus . J Clin Microbiol . 2009 ;47(3) :541 \u2013546 ."},{"issue":"10","key":"i1543-2165-137-1-50-b18","series-title":"Expert Rev Anti Infect Ther","first-page":"1139","article-title":"Commercially available assays for multiplex detection of alpha human papillomaviruses","volume":"8","author":"Poljak\n M ,","year":"2010","unstructured":"Poljak\n M , \n \n Kocjan\n BJ . \n \n Commercially available assays for multiplex detection of alpha human papillomaviruses . Expert Rev Anti Infect Ther . 2010 ;8(10) :1139 \u20131162 ."},{"issue":"4","key":"i1543-2165-137-1-50-b19","series-title":"J Mol Diagn","first-page":"377","article-title":"Efficient DNA extraction for HPV genotyping in formalin-fixed, paraffin-embedded tissues","volume":"13","author":"Steinau\n M ,","year":"2011","unstructured":"Steinau\n M , \n \n Patel\n SS , \n \n Unger\n ER . \n \n Efficient DNA extraction for HPV genotyping in formalin-fixed, paraffin-embedded tissues . J Mol Diagn . 2011 ;13(4) :377 \u2013381 ."},{"issue":"12","key":"i1543-2165-137-1-50-b20","series-title":"Arch Pathol Lab Med","first-page":"1813","article-title":"Use of a linear array for the detection of human papillomavirus genotypes in head and neck cancer","volume":"134","author":"Black\n CC ,","year":"2010","unstructured":"Black\n CC , \n \n Bentley\n HA , \n \n Davis\n TH , \n \n Tsongalis\n GJ . \n \n Use of a linear array for the detection of human papillomavirus genotypes in head and neck cancer . Arch Pathol Lab Med . 2010 ;134(12) :1813 \u20131817 ."},{"issue":"1","key":"i1543-2165-137-1-50-b21","series-title":"J Virol Methods","first-page":"117","article-title":"Comparison of the Abbott RealTime High Risk HPV test and INNO-LiPA HPV Genotyping Extra test for the detection of human papillomaviruses in formalin-fixed, paraffin-embedded cervical cancer specimens","volume":"175","author":"Kocjan\n BJ ,","year":"2011","unstructured":"Kocjan\n BJ , \n \n Seme\n K , \n \n Poljak\n M . \n \n Comparison of the Abbott RealTime High Risk HPV test and INNO-LiPA HPV Genotyping Extra test for the detection of human papillomaviruses in formalin-fixed, paraffin-embedded cervical cancer specimens . J Virol Methods . 2011 ;175(1) :117 \u2013119 ."},{"issue":"4","key":"i1543-2165-137-1-50-b22","series-title":"J Clin Microbiol","first-page":"1458","article-title":"Human papillomavirus genotyping using archival vulval dysplastic or neoplastic biopsy tissues: comparison between the INNO-LiPA and linear array assays","volume":"48","author":"Tan\n SE ,","year":"2010","unstructured":"Tan\n SE , \n \n Garland\n SM , \n \n Rumbold\n AR , \n \n Tabrizi\n SN . \n \n Human papillomavirus genotyping using archival vulval dysplastic or neoplastic biopsy tissues: comparison between the INNO-LiPA and linear array assays . J Clin Microbiol . 2010 ;48(4) :1458 \u20131460 ."},{"key":"i1543-2165-137-1-50-b23","series-title":"Infect Agent Cancer","first-page":"2","article-title":"Rapid, sensitive, type specific PCR detection of the E7 region of human papillomavirus type 16 and 18 from paraffin embedded sections of cervical carcinoma","volume":"5","author":"Lesnikova\n I ,","year":"2010","unstructured":"Lesnikova\n I , \n \n Lidang\n M , \n \n Hamilton-Dutoit\n S , \n \n Koch\n J . \n \n Rapid, sensitive, type specific PCR detection of the E7 region of human papillomavirus type 16 and 18 from paraffin embedded sections of cervical carcinoma . Infect Agent Cancer . 2010 ;5 :2 ."},{"issue":"3","key":"i1543-2165-137-1-50-b24","series-title":"Gynecol Oncol","first-page":"555","article-title":"HPV genotyping in cervical cancer in Northern Thailand: adapting the linear array HPV assay for use on paraffin-embedded tissue","volume":"108","author":"Siriaunkgul\n S ,","year":"2008;","unstructured":"Siriaunkgul\n S , \n \n Suwiwat\n S , \n \n Settakorn\n J , \n et al\n . HPV genotyping in cervical cancer in Northern Thailand: adapting the linear array HPV assay for use on paraffin-embedded tissue . Gynecol Oncol . 2008;108(3) :555 \u2013560 ."},{"issue":"8","key":"i1543-2165-137-1-50-b25","series-title":"J Clin Microbiol","first-page":"3758","article-title":"Comparison of real-time PCR signal-amplified in situ hybridization and conventional PCR for detection and quantification of human papillomavirus in archival cervical cancer tissue","volume":"42","author":"Biedermann\n K ,","year":"2004;","unstructured":"Biedermann\n K , \n \n Dandachi\n N , \n \n Trattner\n M , \n et al\n . Comparison of real-time PCR signal-amplified in situ hybridization and conventional PCR for detection and quantification of human papillomavirus in archival cervical cancer tissue . J Clin Microbiol . 2004;42(8) :3758 \u20133765 ."},{"issue":"10","key":"i1543-2165-137-1-50-b26","series-title":"Am J Surg Pathol","first-page":"1449","article-title":"P16\/CDKN2A and Ki-67 enhance the detection of anal intraepithelial neoplasia and condyloma and correlate with human papillomavirus detection by polymerase chain reaction","volume":"34","author":"Pirog\n EC ,","year":"2010","unstructured":"Pirog\n EC , \n \n Quint\n KD , \n \n Yantiss\n RK . \n \n P16\/CDKN2A and Ki-67 enhance the detection of anal intraepithelial neoplasia and condyloma and correlate with human papillomavirus detection by polymerase chain reaction . Am J Surg Pathol . 2010 ;34(10) :1449 \u20131455 ."},{"issue":"14","key":"i1543-2165-137-1-50-b27","series-title":"J Natl Cancer Inst","first-page":"1072","article-title":"The elevated 10-year risk of cervical precancer and cancer in women with human papillomavirus (HPV) type 16 or 18 and the possible utility of type-specific HPV testing in clinical practice","volume":"97","author":"Khan\n MJ ,","year":"2005;","unstructured":"Khan\n MJ , \n \n Castle\n PE , \n \n Lorincz\n AT , \n et al\n . The elevated 10-year risk of cervical precancer and cancer in women with human papillomavirus (HPV) type 16 or 18 and the possible utility of type-specific HPV testing in clinical practice . J Natl Cancer Inst . 2005;97(14) :1072 \u20131079 ."}],"container-title":["Archives of Pathology & Laboratory Medicine"],"original-title":[],"link":[{"URL":"https:\/\/aplm.kglmeridian.com\/view\/journals\/arpa\/137\/1\/article-p50.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/meridian.allenpress.com\/aplm\/article-pdf\/137\/1\/50\/1610652\/arpa_2011-0392-oa.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/aplm.kglmeridian.com\/downloadpdf\/journals\/arpa\/137\/1\/article-p50.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/aplm.kglmeridian.com\/downloadpdf\/journals\/arpa\/137\/1\/article-p50.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T00:56:07Z","timestamp":1770771367000},"score":1,"resource":{"primary":{"URL":"https:\/\/aplm.kglmeridian.com\/view\/journals\/arpa\/137\/1\/article-p50.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,1]]},"references-count":54,"journal-issue":{"issue":"1"},"URL":"https:\/\/doi.org\/10.5858\/arpa.2011-0392-oa","relation":{},"ISSN":["1543-2165","0003-9985"],"issn-type":[{"value":"1543-2165","type":"electronic"},{"value":"0003-9985","type":"print"}],"subject":[],"published":{"date-parts":[[2013,1]]}}}