{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T05:11:50Z","timestamp":1789708310717,"version":"4.0.1"},"reference-count":29,"publisher":"Archives of Pathology and Laboratory Medicine","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,8,1]]},"abstract":"<jats:sec>\n                    <jats:title>Context.\u2014<\/jats:title>\n                    <jats:p>Infectious gastroenteritis is caused by various pathogens, including bacteria, viruses, and parasites.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective.\u2014<\/jats:title>\n                    <jats:p>To compare the performance of Seegene Allplex Gastrointestinal (24 targets: 13 bacteria, 5 viruses, and 6 parasites in 4 panels), Luminex xTAG Gastrointestinal Pathogen Panel (15 targets: 9 bacteria, 3 viruses, and 3 parasites), and BD MAX Enteric panel (5 bacteria and 3 parasites). We estimated the agreement among 3 molecular assays.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Design.\u2014<\/jats:title>\n                    <jats:p>A total of 858 stool samples (554 bacterial\/parasite and 304 viral pathogens) were included. A consensus positive\/negative was defined as concordant results from at least 2 tests. To evaluate the agreement among the assays, \u03ba value was calculated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results.\u2014<\/jats:title>\n                    <jats:p>The overall positive percentage agreements of Seegene, Luminex, and BD MAX were 94% (258 of 275), 92% (254 of 275), and 78% (46 of 59), respectfully. For Salmonella, Luminex showed low negative percentage agreement because of frequent false positives (n = 31) showing low median fluorescent intensity. For viruses, positive\/negative percentage agreements of Seegene and Luminex were 99%\/96% and 93%\/99%, respectively. Compared with routine microbiology testing, Seegene, Luminex, and BD MAX additionally identified 39, 40, and 12 pathogens, respectively. Sixty-one cases (16 cases with Seegene, 51 cases with Luminex, and 1 case with BD MAX) showed positive results for multiple pathogens, but only 3 were consensus positive.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions.\u2014<\/jats:title>\n                    <jats:p>These multiplex molecular assays appear to be promising tools for the detection and identification of multiple gastrointestinal pathogens simultaneously. However, careful interpretation of positive results for multiple pathogens is required.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.5858\/arpa.2018-0002-oa","type":"journal-article","created":{"date-parts":[[2019,2,14]],"date-time":"2019-02-14T14:14:02Z","timestamp":1550153642000},"page":"999-1005","source":"Crossref","is-referenced-by-count":45,"title":["Comparative Evaluation of Seegene Allplex Gastrointestinal, Luminex xTAG Gastrointestinal Pathogen Panel, and BD MAX Enteric Assays for Detection of Gastrointestinal Pathogens in Clinical Stool Specimens"],"prefix":"10.5858","volume":"143","author":[{"given":"Jaeeun","family":"Yoo","sequence":"first","affiliation":[{"name":"From the Department of Laboratory Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea (Drs Yoo, J. Park, H. K. Lee, and Y.-J. Park); the Department of Laboratory Medicine, Seoul St Mary's Hospital, The Catholic University of Korea, Seoul, Korea (Mss Yu and K. G. Park and Mr G. D. Lee); and the Department of Brain and Cognitive Science, Massachusetts Institute of Technolo"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joonhong","family":"Park","sequence":"additional","affiliation":[{"name":"From the Department of Laboratory Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea (Drs Yoo, J. Park, H. K. Lee, and Y.-J. Park); the Department of Laboratory Medicine, Seoul St Mary's Hospital, The Catholic University of Korea, Seoul, Korea (Mss Yu and K. G. Park and Mr G. D. Lee); and the Department of Brain and Cognitive Science, Massachusetts Institute of Technolo"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hae Kyung","family":"Lee","sequence":"additional","affiliation":[{"name":"From the Department of Laboratory Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea (Drs Yoo, J. Park, H. K. Lee, and Y.-J. Park); the Department of Laboratory Medicine, Seoul St Mary's Hospital, The Catholic University of Korea, Seoul, Korea (Mss Yu and K. G. Park and Mr G. D. Lee); and the Department of Brain and Cognitive Science, Massachusetts Institute of Technolo"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin Kyung","family":"Yu","sequence":"additional","affiliation":[{"name":"From the Department of Laboratory Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea (Drs Yoo, J. Park, H. K. Lee, and Y.-J. Park); the Department of Laboratory Medicine, Seoul St Mary's Hospital, The Catholic University of Korea, Seoul, Korea (Mss Yu and K. G. Park and Mr G. D. Lee); and the Department of Brain and Cognitive Science, Massachusetts Institute of Technolo"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gun Dong","family":"Lee","sequence":"additional","affiliation":[{"name":"From the Department of Laboratory Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea (Drs Yoo, J. Park, H. K. Lee, and Y.-J. Park); the Department of Laboratory Medicine, Seoul St Mary's Hospital, The Catholic University of Korea, Seoul, Korea (Mss Yu and K. G. Park and Mr G. D. Lee); and the Department of Brain and Cognitive Science, Massachusetts Institute of Technolo"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kang Gyun","family":"Park","sequence":"additional","affiliation":[{"name":"From the Department of Laboratory Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea (Drs Yoo, J. Park, H. K. Lee, and Y.-J. Park); the Department of Laboratory Medicine, Seoul St Mary's Hospital, The Catholic University of Korea, Seoul, Korea (Mss Yu and K. G. Park and Mr G. D. Lee); and the Department of Brain and Cognitive Science, Massachusetts Institute of Technolo"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hayeon Caitlyn","family":"Oak","sequence":"additional","affiliation":[{"name":"From the Department of Laboratory Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea (Drs Yoo, J. Park, H. K. Lee, and Y.-J. Park); the Department of Laboratory Medicine, Seoul St Mary's Hospital, The Catholic University of Korea, Seoul, Korea (Mss Yu and K. G. Park and Mr G. D. Lee); and the Department of Brain and Cognitive Science, Massachusetts Institute of Technolo"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yeon-Joon","family":"Park","sequence":"additional","affiliation":[{"name":"From the Department of Laboratory Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea (Drs Yoo, J. Park, H. K. Lee, and Y.-J. Park); the Department of Laboratory Medicine, Seoul St Mary's Hospital, The Catholic University of Korea, Seoul, Korea (Mss Yu and K. G. Park and Mr G. D. Lee); and the Department of Brain and Cognitive Science, Massachusetts Institute of Technolo"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3906","published-online":{"date-parts":[[2019,2,14]]},"reference":[{"issue":"9","key":"2020081203305310800_i1543-2165-143-8-999-b01","doi-asserted-by":"crossref","first-page":"e72788","DOI":"10.1371\/journal.pone.0072788","article-title":"Global causes of diarrheal disease mortality in children &lt;5 years of age: a systematic review","volume":"8","author":"Lanata","year":"2013","journal-title":"PLoS One"},{"issue":"49","key":"2020081203305310800_i1543-2165-143-8-999-b02","doi-asserted-by":"crossref","first-page":"30420","DOI":"10.2807\/1560-7917.ES.2016.21.49.30420","article-title":"Community-wide outbreaks of haemolytic uraemic syndrome associated with Shiga toxin-producing Escherichia coli O26 in Italy and Romania: a new challenge for the European Union","volume":"21","author":"Severi","year":"2016","journal-title":"Euro Surveill"},{"issue":"4","key":"2020081203305310800_i1543-2165-143-8-999-b03","doi-asserted-by":"crossref","first-page":"1222","DOI":"10.1128\/JCM.03099-13","article-title":"Comparison of the BD MAX enteric bacterial panel to routine culture methods for detection of Campylobacter, enterohemorrhagic Escherichia coli (O157), Salmonella, and Shigella isolates in preserved stool specimens","volume":"52","author":"Anderson","year":"2014","journal-title":"J Clin Microbiol"},{"issue":"2","key":"2020081203305310800_i1543-2165-143-8-999-b04","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.diagmicrobio.2014.02.004","article-title":"Evaluation of a multiplex PCR assay for simultaneous detection of bacterial and viral enteropathogens in stool samples of paediatric patients","volume":"79","author":"Onori","year":"2014","journal-title":"Diagn Microbiol Infect Dis"},{"issue":"3","key":"2020081203305310800_i1543-2165-143-8-999-b05","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1128\/JCM.02674-14","article-title":"Multicenter evaluation of the BioFire FilmArray gastrointestinal panel for etiologic diagnosis of infectious gastroenteritis","volume":"53","author":"Buss","year":"2015","journal-title":"J Clin Microbiol"},{"key":"2020081203305310800_i1543-2165-143-8-999-b06","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.ijid.2015.02.011","article-title":"Comparative evaluation of the new xTAG GPP multiplex assay in the laboratory diagnosis of acute gastroenteritis: clinical assessment and potential application from a multicentre Italian study","volume":"34","author":"Vocale","year":"2015","journal-title":"Int J Infect Dis"},{"issue":"9","key":"2020081203305310800_i1543-2165-143-8-999-b07","doi-asserted-by":"crossref","first-page":"2262","DOI":"10.1128\/JCM.00558-16","article-title":"Evaluation of a multiplex real-time PCR assay for detecting major bacterial enteric pathogens in fecal specimens: intestinal inflammation and bacterial load are correlated in Campylobacter infections","volume":"54","author":"Wohlwend","year":"2016","journal-title":"J Clin Microbiol"},{"key":"2020081203305310800_i1543-2165-143-8-999-b08","unstructured":"Clinical and Laboratory Standards Institute. 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