{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T00:51:14Z","timestamp":1793407874260,"version":"4.5.0"},"reference-count":30,"publisher":"Archives of Pathology and Laboratory Medicine","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,5,1]]},"abstract":"<jats:sec>\n                    <jats:title>Context.\u2014<\/jats:title>\n                    <jats:p>The improved survival and better response to treatments of human papillomavirus (HPV)\u2013related oropharyngeal squamous cell carcinoma (OPSCC) highlight the need for effective tools in evaluating HPV status on formalin-fixed, paraffin-embedded (FFPE) cancer tissues. To date, there is no agreement regarding the most appropriate method for HPV testing on FFPE materials.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective.\u2014<\/jats:title>\n                    <jats:p>We aimed to investigate the performance of the Anyplex II HPV28 (Anyplex) on FFPE OPSCC tissues and to compare it with 2 other methods for HPV-DNA detection and p16 overexpression.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Design.\u2014<\/jats:title>\n                    <jats:p>One hundred sixty FFPE OPSCCs were evaluated, which had already been analyzed with the INNO-LiPA HPV assay, Xpert HPV assay, and p16 immunostaining.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results.\u2014<\/jats:title>\n                    <jats:p>All the samples but 1 provided valid results with the Anyplex, which showed the highest HPV detection rate and a good concordance with all the other methods (\u03ba = 0.75, 95% CI, 0.65\u20130.85 versus INNO-LiPA; \u03ba = 0.80, 95% CI, 0.70\u20130.89 versus Xpert; \u03ba = 0.76, 95% CI, 0.65\u20130.86 versus p16). Moreover, the HPV-driven fraction, based on HPV-DNA and p16 double positivity, was higher with Anyplex (83 of 159, 52.2%) than with the other 2 assays, that is, 78 of 156 (50.0%) for INNO-LiPA and 80 of 160 (50.0%) for Xpert.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions.\u2014<\/jats:title>\n                    <jats:p>Anyplex II HPV28 showed a higher HPV detection rate and HPV-associated fraction than the other methods used. This assay is suitable for HPV detection in archival OPSCC tissues.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.5858\/arpa.2019-0199-oa","type":"journal-article","created":{"date-parts":[[2019,9,11]],"date-time":"2019-09-11T13:07:26Z","timestamp":1568207246000},"page":"620-625","source":"Crossref","is-referenced-by-count":15,"title":["Evaluation of the Anyplex II HPV28 Assay in the Detection of Human Papillomavirus in Archival Samples of Oropharyngeal Carcinomas"],"prefix":"10.5858","volume":"144","author":[{"given":"Francesca","family":"Rollo","sequence":"first","affiliation":[{"name":"From the Departments of Pathology (Drs Rollo, Covello, and Benevolo) and Otolaryngology Head Neck Surgery (Drs Pichi and Pellini), Regina Elena National Cancer Institute IRCCS, Rome, Italy; and STI\/HIV Unit, San Gallicano Dermatologic Institute IRCCS, Rome, Italy (Dr Don\u00e0)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Gabriella","family":"Don\u00e0","sequence":"additional","affiliation":[{"name":"From the Departments of Pathology (Drs Rollo, Covello, and Benevolo) and Otolaryngology Head Neck Surgery (Drs Pichi and Pellini), Regina Elena National Cancer Institute IRCCS, Rome, Italy; and STI\/HIV Unit, San Gallicano Dermatologic Institute IRCCS, Rome, Italy (Dr Don\u00e0)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Pichi","sequence":"additional","affiliation":[{"name":"From the Departments of Pathology (Drs Rollo, Covello, and Benevolo) and Otolaryngology Head Neck Surgery (Drs Pichi and Pellini), Regina Elena National Cancer Institute IRCCS, Rome, Italy; and STI\/HIV Unit, San Gallicano Dermatologic Institute IRCCS, Rome, Italy (Dr Don\u00e0)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raul","family":"Pellini","sequence":"additional","affiliation":[{"name":"From the Departments of Pathology (Drs Rollo, Covello, and Benevolo) and Otolaryngology Head Neck Surgery (Drs Pichi and Pellini), Regina Elena National Cancer Institute IRCCS, Rome, Italy; and STI\/HIV Unit, San Gallicano Dermatologic Institute IRCCS, Rome, Italy (Dr Don\u00e0)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renato","family":"Covello","sequence":"additional","affiliation":[{"name":"From the Departments of Pathology (Drs Rollo, Covello, and Benevolo) and Otolaryngology Head Neck Surgery (Drs Pichi and Pellini), Regina Elena National Cancer Institute IRCCS, Rome, Italy; and STI\/HIV Unit, San Gallicano Dermatologic Institute IRCCS, Rome, Italy (Dr Don\u00e0)."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Benevolo","sequence":"additional","affiliation":[{"name":"From the Departments of Pathology (Drs Rollo, Covello, and Benevolo) and Otolaryngology Head Neck Surgery (Drs Pichi and Pellini), Regina Elena National Cancer Institute IRCCS, Rome, Italy; and STI\/HIV Unit, San Gallicano Dermatologic Institute IRCCS, Rome, Italy (Dr Don\u00e0)."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3906","published-online":{"date-parts":[[2019,9,11]]},"reference":[{"issue":"6","key":"2020081300293654000_i1543-2165-144-5-620-b01","doi-asserted-by":"crossref","first-page":"djv403","DOI":"10.1093\/jnci\/djv403","article-title":"HPV Involvement in head and neck cancers: comprehensive assessment of biomarkers in 3680 patients","volume":"108","author":"Castellsague","year":"2016","journal-title":"J Natl Cancer Inst"},{"key":"2020081300293654000_i1543-2165-144-5-620-b02","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.pvr.2017.05.002","article-title":"Time to change perspectives on HPV in oropharyngeal cancer: a systematic review of HPV prevalence per oropharyngeal sub-site the last 3 years","volume":"4","author":"Haeggblom","year":"2017","journal-title":"Papillomavirus Res"},{"issue":"12","key":"2020081300293654000_i1543-2165-144-5-620-b03","doi-asserted-by":"crossref","first-page":"1319","DOI":"10.1016\/S1470-2045(14)70471-1","article-title":"HPV DNA, E6\/E7 mRNA, and p16INK4a detection in head and neck cancers: a systematic review and meta-analysis","volume":"15","author":"Ndiaye","year":"2014","journal-title":"Lancet Oncol"},{"key":"2020081300293654000_i1543-2165-144-5-620-b04","unstructured":"International Agency Research Cancer. 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