{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T17:54:32Z","timestamp":1791309272276,"version":"4.3.1"},"reference-count":0,"publisher":"GN1 Sistemas e Publicacoes Ltd.","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JBRA"],"published-print":{"date-parts":[[2026]]},"abstract":"<jats:p>Objective: Testicular torsion (TT) may cause testicular necrosis. Although restoration of blood flow is essential to prevent testicular loss, reperfusion may induce testicular ischemia-reperfusion injury (TIRI), leading to testicular damage and potentially long-term infertility. All-trans retinoic acid (ATRA) has been shown to enhance the transcription of antioxidant enzymes and anti-apoptotic proteins, inhibit NF-\u03baB-mediated transcription of inflammatory cytokines, and support germ-cell differentiation and spermatogenesis. This study investigated the protective effect of ATRA on the ipsilateral testis following unilateral testicular torsion\/detorsion.\nMethods: Twenty-four male Wistar rats were divided into four groups (n=6): Sham, Torsion\/Detorsion (TD), ATRA+TD (7.5mg\/kg administered 3 days before TIRI induction), and TD+ATRA (7.5mg\/kg administered 3 days after TIRI induction). Torsion was induced for 1 hour and was followed immediately by detorsion.\nResults: TD reduced the levels of reproductive hormones (testosterone and inhibin), Bcl-2, sperm indices, SOD, CAT, GPx, GST, GSH, and total protein, with concomitant increases in testicular MDA, NO, TNF-\u03b1, MPO, IL-6, NF-\u03baB, Bax, caspase-3, DFI, and 8-OHdG levels. Histological evaluation of TD rats revealed reduced spermatogenesis indices, disruption of the seminiferous tubules, germ-cell loss, and vascular congestion. ATRA treatment significantly improved antioxidant enzyme activity, downregulated NF-\u03baB and Bax signaling, restored reproductive hormones and sperm parameters, and protected testicular and epididymal structures.\nConclusion: ATRA protects against ipsilateral testicular damage following torsion\/detorsion, as shown by restoration of depleted antioxidant enzymes, downregulation of inflammatory and pro-apoptotic markers, and enhancement of spermatogenesis and hormonal balance. These findings support the potential role of ATRA in preserving fertility after testicular torsion\/detorsion.<\/jats:p>","DOI":"10.5935\/1518-0557.20260059","type":"journal-article","created":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T17:33:15Z","timestamp":1791307995000},"page":"1-15","source":"Crossref","is-referenced-by-count":0,"title":["Protective Role of All-Trans Retinoic Acid Against Ipsilateral Testicular Ischemia-Reperfusion Injury in Male Wistar Rats"],"prefix":"10.5935","volume":"30","author":[{"given":"Richard","family":"Ajike","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oladele A","family":"folabi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oluwaseun","family":"Hezekiah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ezekiel","family":"Ojo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olajide","family":"Amao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorcas","family":"Akinyemi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bamidele","family":"Olusola","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Onaopepo","family":"Lawal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peace","family":"Adekunleoke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3948","published-online":{"date-parts":[[2026]]},"container-title":["JBRA Assisted Reproduction"],"original-title":[],"link":[{"URL":"https:\/\/www.jbra.com.br\/trab\/pub\/download_trabalho.php?fileSource=\/var\/www\/vhosts\/jbra.com.br\/media\/trab\/arq_8823&fileName=3497.pdf&id_trabalho=3301","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T17:33:20Z","timestamp":1791308000000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jbra.com.br\/trab\/pub\/download_trabalho.php?fileSource=\/var\/www\/vhosts\/jbra.com.br\/media\/trab\/arq_8823&fileName=3497.pdf&id_trabalho=3301"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"references-count":0,"URL":"https:\/\/doi.org\/10.5935\/1518-0557.20260059","relation":{},"ISSN":["1518-0557"],"issn-type":[{"value":"1518-0557","type":"print"}],"subject":[],"published":{"date-parts":[[2026]]}}}