{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T17:53:53Z","timestamp":1791309233433,"version":"4.3.1"},"reference-count":0,"publisher":"GN1 Sistemas e Publicacoes Ltd.","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JBRA"],"published-print":{"date-parts":[[2026]]},"abstract":"<jats:p>Objective: To evaluate the association between embryo morphology at the cleavage stage (Day 3) and the blastocyst stage and the likelihood of euploidy.\nMethods: This retrospective cross-sectional study included 519 embryos from 250 patients who underwent controlled ovarian stimulation with either oral progestins or gonadotropin-releasing hormone (GnRH) antagonists, followed by intracytoplasmic sperm injection (ICSI) and preimplantation genetic testing for aneuploidy (PGT-A), between 2018 and 2023. Day-3 embryos were classified according to cell number (&lt;8 vs. \u22658) and fragmentation grade (A, B, or C, according to the 2011 Istanbul Consensus). Blastocysts were graded using the Gardner classification and categorized as top quality (\u22653BB) or non-top quality. Associations between morphology and ploidy were analyzed using the chi-square test or Fisher\u2019s exact test, as appropriate, and expressed as odds ratios (ORs) with 95% confidence intervals (CIs).\nResults: Among the 519 embryos, 140 (26.9%) were euploid and 379 (73.1%) were aneuploid. Embryos with \u22658 cells on Day 3 exhibited significantly less fragmentation (p&lt;0.001) and were more likely to develop into top-quality blastocysts (50.1% vs. 37.7%, p=0.002). Top-quality blastocysts had nearly twice the euploidy rate of non-top-quality blastocysts (36.9% vs. 18.7%, p&lt;0.001). No significant association was found between Day-3 cell number and euploidy (p=0.550).\nConclusions: Embryos with \u22658 cells on Day 3 were more likely to develop into top-quality blastocysts and exhibited less fragmentation. Although blastocyst morphology was associated with euploidy, Day-3 parameters alone were not predictive. These findings highlight the importance of combining morphological and genetic assessments for embryo selection.<\/jats:p>","DOI":"10.5935\/1518-0557.20260065","type":"journal-article","created":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T17:33:15Z","timestamp":1791307995000},"page":"1-7","source":"Crossref","is-referenced-by-count":0,"title":["Embryo Morphology and Euploidy Rate: A Cross-Sectional Analysis of Day-3 and Blastocyst-Stage Quality"],"prefix":"10.5935","volume":"30","author":[{"given":"Giovanna","family":"Giovacchini","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulo Victor Zattar","family":"Ribeiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caina Goncalves","family":"Rodrigues","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline Castrucci","family":"Ingold","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna Luiza Braga","family":"Albuquerque","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caio Parente","family":"Barbosa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renato","family":"Oliveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3948","published-online":{"date-parts":[[2026]]},"container-title":["JBRA Assisted Reproduction"],"original-title":[],"link":[{"URL":"https:\/\/www.jbra.com.br\/trab\/pub\/download_trabalho.php?fileSource=\/var\/www\/vhosts\/jbra.com.br\/media\/trab\/arq_8827&fileName=3408%20-%20Embryo%20Morphology%20and%20Euploidy%20Rate%20A%20Cross-Sectional%20Analysis%20of%20Day-3%20and%20Blastocyst-Stage%20Quality.pdf&id_trabalho=3429","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T17:33:16Z","timestamp":1791307996000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.jbra.com.br\/trab\/pub\/download_trabalho.php?fileSource=\/var\/www\/vhosts\/jbra.com.br\/media\/trab\/arq_8827&fileName=3408%20-%20Embryo%20Morphology%20and%20Euploidy%20Rate%20A%20Cross-Sectional%20Analysis%20of%20Day-3%20and%20Blastocyst-Stage%20Quality.pdf&id_trabalho=3429"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"references-count":0,"URL":"https:\/\/doi.org\/10.5935\/1518-0557.20260065","relation":{},"ISSN":["1518-0557"],"issn-type":[{"value":"1518-0557","type":"print"}],"subject":[],"published":{"date-parts":[[2026]]}}}