{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T19:57:54Z","timestamp":1790539074034,"version":"4.1.0"},"reference-count":15,"publisher":"Centre for Evaluation in Education and Science (CEON\/CEES)","issue":"2","license":[{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/BY\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Med Biochemistry"],"published-print":{"date-parts":[[2021]]},"abstract":"<jats:p>Background: Accurate diagnosis and classification of von Willebrand disease (VWD) are essential for optimal management. The von Willebrand factor multimers analysis (VWF:MM) is an integral part of the diagnostic process in the phenotypic classification, especially in discrepant cases. The aim of this study was to evaluate the performance of a new Hydragel 11VWF multimer assay (H11VW). Methods: Analytical performance characteristics such as repeatability (intra-assay variability, in gel between track variation), reproducibility (inter-assay variability, between gel variation), sensitivity, EQA performance and differences between two commercially available VWF:MM kits (H5VW and H11VW) were analysed in healthy volunteers' plasmas using in-house prepared reference plasma. Results: Repeatability and reproducibility results of H11VW demonstrated acceptable and equivalent performance with previously verified H5VW. Participation in EQA was successful. No statistically significant difference was detected between H5VW and H11VW kits for different fractions of multimers: LMWM p=0.807; IMWM p=0.183; HMWM p=0.774. Conclusions: H11VW demonstrated acceptable analytical performance characteristics. H11VW kit conveniently offers a more significant number of samples on a single gel. H5VW and H11VW kits can be used in daily practice interchangeably.<\/jats:p>","DOI":"10.5937\/jomb0-26008","type":"journal-article","created":{"date-parts":[[2020,8,18]],"date-time":"2020-08-18T10:21:50Z","timestamp":1597746110000},"page":"167-172","source":"Crossref","is-referenced-by-count":8,"title":["Evaluation of a new semi-automated Hydragel 11 von Willebrand factor multimers assay kit for routine use"],"prefix":"10.5937","volume":"40","author":[{"given":"Marika","family":"Pikta","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timea","family":"Szanto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margus","family":"Viigimaa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Lejniece","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D\u0101rta","family":"Balode","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kadri","family":"Saks","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valdas","family":"Banys","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3964","reference":[{"key":"ref1","doi-asserted-by":"crossref","unstructured":"Favaloro EJ. Von Willebrand disease: local diagnosis and management of a globally distributed bleeding disorder. Semin Thromb Hemost 2011; 37(5): 440-55;","DOI":"10.1055\/s-0031-1281028"},{"key":"ref2","doi-asserted-by":"crossref","unstructured":"Szanto T, Lassila R, Funding E, Onundarson PT, Strand - berg K, Baghaei F on behalf of the Nordic Haemophilia Council. Von Willebrand disease -the Nordic perspective. Ann Blood 2018; 3: 2;","DOI":"10.21037\/aob.2017.12.07"},{"key":"ref3","doi-asserted-by":"crossref","unstructured":"Nummi V, Lassila R, Joutsi-KorhonenL, Armstrong E, Szanto T. Comprehensive re-evaluation of historical von Willebrand disease diagnosis in association with whole blood platelet aggregation and function. Int J Lab Hem 2018; 40: 304 -11;","DOI":"10.1111\/ijlh.12785"},{"key":"ref4","doi-asserted-by":"crossref","unstructured":"Roberts JC, Flood VH. Laboratory diagnosis of von Willebrand disease. Int J Lab Hematol 2015; 37(Suppl): 11-7;","DOI":"10.1111\/ijlh.12345"},{"key":"ref5","doi-asserted-by":"crossref","unstructured":"Torres R, Genzen JR, Levene MJ. Clinical Measurement of von Willebrand Factor by Fluorescence Correlation Spectroscopy. Clin Chem 2012; 58: 1010 -8;","DOI":"10.1373\/clinchem.2011.179200"},{"key":"ref6","doi-asserted-by":"crossref","unstructured":"Meijer P, Haverkate F. An external quality assessment program for von Willebrand factor laboratory analysis: an overview from the European concerted action on thrombosis and disabilities foundation. Semin Thromb Hemost 2006; 32: 485-91;","DOI":"10.1055\/s-2006-947862"},{"key":"ref7","doi-asserted-by":"crossref","unstructured":"Ledford-Kraemer MR. Analysis of von Willebrand factor structure by multimer analysis. Am J Hematol 2010; 85: 510-4;","DOI":"10.1002\/ajh.21739"},{"key":"ref8","doi-asserted-by":"crossref","unstructured":"Oliver S, Lau KKE, Chapman K, Favaloro EJ. Laboratory testing for von Willebrand factor multimers. Methods Mol Biol 2017; 1646: 495-511;","DOI":"10.1007\/978-1-4939-7196-1_36"},{"key":"ref9","doi-asserted-by":"crossref","unstructured":"Crist RA, Heikal NM, Rodgers GM, Grenache DG, Smock KJ. Evaluation of a new commercial method for von Willebrand factor multimeric analysis. Int J Lab Hem 2018; 40: 586-91;","DOI":"10.1111\/ijlh.12869"},{"key":"ref10","doi-asserted-by":"crossref","unstructured":"Favaloro EJ, Oliver S. Evaluation of a new commercial von Willebrand factor multimer assay. Haemophilia. 2017; 23: e373-7;","DOI":"10.1111\/hae.13261"},{"key":"ref11","doi-asserted-by":"crossref","unstructured":"Pikta M, Zemtsovskaja G, Bautista H, Nouadje G, Szanto T, Viigimaa M, et al. Preclinical evaluation of a semi-automated and rapid commercial electrophoresis assay for von Willebrand factor multimers. J Clin Lab Anal 2018; 32: e22416;","DOI":"10.1002\/jcla.22416"},{"key":"ref12","doi-asserted-by":"crossref","unstructured":"Bowyer AE, Goodfellow KJ, Seidel H, Westhofen P, Stufano F, Goodeve A, et al. Evaluation of a semi-automated von Willebrand factor multimer assay, the Hydragel 5 von Willebrand multimer, by two European Centers. Res Pract Thromb Haemost 2018; 2: 790-9;","DOI":"10.1002\/rth2.12141"},{"key":"ref13","unstructured":"Pikta M, Zolotareva V, T\u00f5nne J, Viigimaa M, Banys V. Implementation and Verification of New VWF: Ac Assay System with Components from Different Manufacturers. Laboratorine medicina 2016; 72: 185-8;"},{"key":"ref14","doi-asserted-by":"crossref","unstructured":"Favaloro EJ. Rethinking internal quality control and external quality assessment for laboratory diagnostics of von Willebrand disease. Ann Blood 2019; 4: 4;","DOI":"10.21037\/aob.2019.01.03"},{"key":"ref15","doi-asserted-by":"crossref","unstructured":"Oliver, S, Vanniasinkam, T, Mohammed, S, Vong, R, Favaloro, EJ. Semi-automated von Willebrand factor multimer assay for von Willebrand disease: Further validation, benefits and limitations. Int J Lab Hem 2019; 41(6): 762-71;","DOI":"10.1111\/ijlh.13107"}],"container-title":["Journal of Medical Biochemistry"],"original-title":["Procena novog poluautomatskog seta za testiranje 11 fon Vildenbrandovog faktora za rutinsku upotrebu"],"language":"en","link":[{"URL":"https:\/\/aseestant.ceon.rs\/index.php\/jomb\/article\/download\/26008\/15796","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/scindeks-clanci.ceon.rs\/data\/pdf\/1452-8258\/2021\/1452-82582102167P.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,3,12]],"date-time":"2021-03-12T20:47:04Z","timestamp":1615582024000},"score":1,"resource":{"primary":{"URL":"https:\/\/scindeks.ceon.rs\/Article.aspx?artid=1452-82582102167P"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021]]},"references-count":15,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2021]]}},"URL":"https:\/\/doi.org\/10.5937\/jomb0-26008","relation":{},"ISSN":["1452-8258","1452-8266"],"issn-type":[{"value":"1452-8258","type":"print"},{"value":"1452-8266","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021]]}}}