{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T15:15:04Z","timestamp":1767626104791,"version":"3.41.0"},"reference-count":21,"publisher":"Centre for Evaluation in Education and Science (CEON\/CEES)","issue":"4","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/BY\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Med Biochemistry"],"published-print":{"date-parts":[[2025]]},"abstract":"<jats:p>Background: The analytical performance metrics of ethanol testing are critically important due to their legal implications when presenting and interpreting results. Measurement uncertainty (MU) and total analytical error (TAE) are essential approaches for evaluating and improving the quality of measurement procedures. This study aimed to calculate MU and TAE values, which assess the reliability of ethanol test results from different perspectives, and to evaluate the impact of MU values, calculated using two different methods, on the legal threshold. Methods: MU values were calculated following the guidelines of Nordtest and ISO\/TS 20914. TAE was determined using the formula TAE%=1.65\u00d7CV%+Bias%. External and internal quality data from ethanol testing conducted between July 1, 2022, and June 30, 2024, were used for calculations. Results: The expanded MU values for ethanol testing were 13.95% according to the Nordtest Guide, 10.94% for low level and 9.59% for high level according to the ISO\/TS 20914 Guide. The calculated TAE values were 12.59 for low levels, 11.47 for high levels, and 12.57 overall. MU and TAE values for ethanol testing in our laboratory remained within the allowable total error (\u00b120%) according to CLIA 2024. Conclusions: We believe each laboratory should report ethanol test results and their respective MU values, particularly when evaluating results close to legal thresholds. Furthermore, we suggest that scientific committees standardise the method for calculating MU and define a target limit.<\/jats:p>","DOI":"10.5937\/jomb0-55093","type":"journal-article","created":{"date-parts":[[2025,3,10]],"date-time":"2025-03-10T10:13:42Z","timestamp":1741601622000},"page":"808-813","source":"Crossref","is-referenced-by-count":1,"title":["Evaluation of measurement uncertainty results calculated by two different methods and total analytical error for ethanol testing"],"prefix":"10.5937","volume":"44","author":[{"suffix":"Akif","given":"Mehmet","family":"Bozdayi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Gamze","given":"\u0130smet","family":"Bozday\u0131","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G\u00f6khan","family":"\u00c7ak\u0131rca","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"3964","reference":[{"key":"ref1","doi-asserted-by":"crossref","unstructured":"Harris JC, Leggio L, Farokhnia M. 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Nordtest Report TR 537 (ed. 4) 2017.;"},{"key":"ref11","unstructured":"International Organization for Standardization. ISO\/TS 20914. Medical Laboratories-Practical Guidance for the Estimation of Measurement Uncertainty. Geneva, Switzerland.;"},{"key":"ref12","doi-asserted-by":"crossref","unstructured":"Lippi G, Mattiuzzi C, Favaloro EJ. Artificial intelligence in the pre-analytical phase: State-of-the art and future perspectives. J Med Biochem 2024; 43 (1): 1-10.;","DOI":"10.5937\/jomb0-45936"},{"key":"ref13","doi-asserted-by":"crossref","unstructured":"\u00c7ubuk\u00e7u HC, Yavuz \u00d6, Devrim E. Uncertainty of measurement for 14 immunoassay analytes: application to laboratory result interpretation. Scand J Clin Lab Invest 2019; 79(1-2): 117-22.;","DOI":"10.1080\/00365513.2018.1550806"},{"key":"ref14","doi-asserted-by":"crossref","unstructured":"Ceriotti F. Deriving proper measurement uncertainty from Internal Quality Control data: An impossible mission? Clin Biochem 2018; 57: 37-40.;","DOI":"10.1016\/j.clinbiochem.2018.03.019"},{"key":"ref15","doi-asserted-by":"crossref","unstructured":"Westgard JO, Westgard SA. Measuring Analytical Quality: Total Analytical Error Versus Measurement Uncertainty. Clin Lab Med 2017; 37(1): 1-13.;","DOI":"10.1016\/j.cll.2016.09.001"},{"key":"ref16","unstructured":"2024 CLIA Acceptance Limits for Proficiency Testing Available from: https:\/\/westgard.com\/clia-a-quality\/quality-requirements\/2024-clia-requirements.html.;"},{"key":"ref17","doi-asserted-by":"crossref","unstructured":"Tan R, Yilmaz M, Kurtulmu\u015f Y. A practical approach example to measurement uncertainty: Evaluation of 26 immunoassay parameters. Biochem Med (Zagreb); 2022; 32(3): 030705.;","DOI":"10.11613\/BM.2022.030705"},{"key":"ref18","unstructured":"Catak Z, Ko\u00e7demir E. Evaluation of Uncertainty in Serum Ethanol Measurement. Journal of Turkish Clinical Biochemistry 2017; 15(2): 75-9.;"},{"key":"ref19","doi-asserted-by":"crossref","unstructured":"Erdogan S, Sen O, Bal C, Oztas D, Avcioglu G, Baran P, et al. Reporting measurement uncertainties with ethanol results. Turk J Biochem 2020; 45(3): 255-61.;","DOI":"10.1515\/tjb-2018-0508"},{"key":"ref20","doi-asserted-by":"crossref","unstructured":"Nurlu N, Cat A, Ucar KT. Measurement uncertainty in clinical chemistry: ISO 20914 versus nordtest or intermediate precision versus bias. Scand J Clin Lab Invest 2024; 84(3): 147-53.;","DOI":"10.1080\/00365513.2024.2338738"},{"key":"ref21","unstructured":"CLSI. Statistical Quality Control for Quantitative Measurement Procedures: Principles and Definitions. 4th ed. CLSI guideline C24. Wayne, PA: Clinical and Laboratory Standards Institute; 2016.;"}],"container-title":["Journal of Medical Biochemistry"],"original-title":["Evaluacija rezultata merne nesigurnosti izra\u010dunatih dvema razli\u010ditim metodama i ukupne analiti\u010dke gre\u0161ke pri testiranju etanola"],"language":"en","link":[{"URL":"https:\/\/scindeks-clanci.ceon.rs\/data\/pdf\/1452-8258\/2025\/1452-82582504808B.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,4]],"date-time":"2025-07-04T14:49:56Z","timestamp":1751640596000},"score":1,"resource":{"primary":{"URL":"https:\/\/scindeks.ceon.rs\/Article.aspx?artid=1452-82582504808B"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025]]},"references-count":21,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025]]}},"URL":"https:\/\/doi.org\/10.5937\/jomb0-55093","relation":{},"ISSN":["1452-8258","1452-8266"],"issn-type":[{"type":"print","value":"1452-8258"},{"type":"electronic","value":"1452-8266"}],"subject":[],"published":{"date-parts":[[2025]]}}}