{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T13:17:06Z","timestamp":1767705426135,"version":"3.48.0"},"reference-count":20,"publisher":"Centre for Evaluation in Education and Science (CEON\/CEES)","issue":"9","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/BY\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Med Biochemistry"],"published-print":{"date-parts":[[2025]]},"abstract":"<jats:p>Background: To explore the correlation of sperm DNA fragmentation index (DFI) with semen quality, while assessing the diagnostic potential of DFI in male infertility, aiming to offer a novel biomarker and clinical approach for male fertility evaluation. Methods: A cohort of 613 men who visited our hospital between April 2023 and February 2025 was included in this study. Semen analysis (assessing concentration, motility, morphology, etc.) and sperm chromatin dispersion testing were conducted to determine DFI. The diagnostic performance of DFI for infertility was evaluated using receiver operating characteristic (ROC) curve analysis. Subgroup analyses (oligozoospermia, asthenozoospermia, and oligoasthenozoospermia) were conducted to assess the discriminative power of DFI. After treatment, the patients were followed up for 1 year, and the predictive effect of DFI on the prognosis of successful fertility was analysed. Results: Among infertile men (n = 92, incidence rate: 15.01%), DFI levels were significantly elevated compared to those with normal fertility (P&amp;lt; 0.05). ROC curve analysis demonstrated that DFI had a sensitivity of 60.87% and specificity of 84.07% (AUC= 0.774) in diagnosing infertility. Notably, DFI displayed the highest discriminative efficacy for oligoasthenozoospermia (AUC= 0.825). Finally, the sensitivity and specificity of DFI for predicting successful fertility in infertile men at 1 year were 83.08% and 62.96%, respectively (P&amp;lt; 0.001). Conclusions: DFI demonstrates high diagnostic accuracy on the fertility of infertile men and has a high clinical potential.<\/jats:p>","DOI":"10.5937\/jomb0-59605","type":"journal-article","created":{"date-parts":[[2025,7,18]],"date-time":"2025-07-18T07:52:40Z","timestamp":1752825160000},"page":"1976-1983","source":"Crossref","is-referenced-by-count":0,"title":["Diagnostic accuracy of sperm DNA fragmentation index in male infertility: A cohort study"],"prefix":"10.5937","volume":"44","author":[{"given":"Juan","family":"Zhang","sequence":"first","affiliation":[{"name":"Jiuquan Branch of Shanghai First People's Hospital, Department of Reproductive Medicine, Jiuquan, Gansu, 735000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"He","sequence":"additional","affiliation":[{"name":"Jiuquan Branch of Shanghai First People's Hospital, Department of Reproductive Medicine, Jiuquan, Gansu, 735000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"3964","reference":[{"key":"ref1","doi-asserted-by":"crossref","unstructured":"Eisenberg ML, Esteves SC, Lamb DJ, Hotaling JM, Giwercman A, Hwang K, et al. Male infertility. Nat Rev Dis Primers 2023; 9(1): 49;","DOI":"10.1038\/s41572-023-00459-w"},{"key":"ref2","doi-asserted-by":"crossref","unstructured":"Rama N, Lescay H, Raheem O. Male Factor Infertility: What Every OB\/GYN Should Know. Obstet Gynecol Clin North Am 2023; 50(4): 763-77;","DOI":"10.1016\/j.ogc.2023.08.001"},{"key":"ref3","unstructured":"Fedder J, Carlsen E, Jorgensen N, Jensen CFS. Treatment of male infertility. Ugeskr Laeger 2021; 183(48);"},{"key":"ref4","doi-asserted-by":"crossref","unstructured":"Andrabi SW, Ara A, Saharan A, Jaffar M, Gugnani N, Esteves SC. Sperm DNA Fragmentation: causes, evaluation and management in male infertility. JBRA Assist Reprod 2024; 28(2): 306-19;","DOI":"10.5935\/1518-0557.20230076"},{"key":"ref5","doi-asserted-by":"crossref","unstructured":"Birowo P Rahendra Wijaya J, Atmoko W, Rasyid N. The effects of varicocelectomy on the DNA fragmentation index and other sperm parameters: a meta-analysis. Basic Clin Androl 2020; 30: 15;","DOI":"10.1186\/s12610-020-00112-6"},{"key":"ref6","doi-asserted-by":"crossref","unstructured":"Pakpahan C, Utomo NS, Hartanto MC, Torra-Massana M, Rezano A. Meta-correlation of Sperm Morphology and DNA Fragmentation Index Reprod Sci. 2025;","DOI":"10.1007\/s43032-025-01867-x"},{"key":"ref7","doi-asserted-by":"crossref","unstructured":"Szabo A, Vancsa S, Hegyi P Varadi A, Forintos A, Filipov T, et al. Lifestyle-, environmental-, and additional health factors associated with an increased sperm DNA fragmentation: a systematic review and meta-analysis. Reprod Biol Endocrinol 2023; 21(1): 5;","DOI":"10.1186\/s12958-023-01054-0"},{"key":"ref8","doi-asserted-by":"crossref","unstructured":"Lin HT, Wu MH, Wu WL, Tsai LC, Chen YY, Hung KH, et al. Incorporating sperm DNA fragmentation index with computer-assisted semen morphokinematic parameters as a better window to male fertility. Chin J Physiol 2022; 65(3): 143-50;","DOI":"10.4103\/CJP.CJP_12_22"},{"key":"ref9","doi-asserted-by":"crossref","unstructured":"Akhavizadegan H, Yamini N, Musavi AM, Moradi M, Khatami F Sperm DNA Fragmentation Index in Abortion or in Vitro Fertilization Failure in Presence of Normal Semen Analysis. Prague Med Rep 2023; 124(2): 166-71;","DOI":"10.14712\/23362936.2023.12"},{"key":"ref10","doi-asserted-by":"crossref","unstructured":"Minhas S, Bettocchi C, Boeri L, Capogrosso P Carvalho J, Cilesiz NC, et al. European Association of Urology Guidelines on Male Sexual and Reproductive Health: 2021 Update on Male Infertility. Eur Urol 2021; 80(5): 603-20;","DOI":"10.1016\/j.eururo.2021.08.014"},{"key":"ref11","doi-asserted-by":"crossref","unstructured":"Feng J, He H, Wang Y Zhang X , Zhang X , Zhang T et al. The efficacy and mechanism of acupuncture in the treatment of male infertility: A literature review. Front Endocrinol (Lausanne) 2022; 13: 1009537;","DOI":"10.3389\/fendo.2022.1009537"},{"key":"ref12","doi-asserted-by":"crossref","unstructured":"Rajovski S, Matijasevic-Jokovic S, Milanovic N, Radovanovic N, Brkusanin M, Savic-Pavicevic D, et al. Association between genetic variants in hsa-miR-27a and hsa-miR-146a genes and male infertility. J Med Biochem 2024; 43(6): 936-45;","DOI":"10.5937\/jomb0-50876"},{"key":"ref13","doi-asserted-by":"crossref","unstructured":"Takalani NB, Monageng EM, Mohlala K, Monsees TK, Henkel R, Opuwari CS. Role of oxidative stress in male infertility. Reprod Fertil 2023; 4(3);","DOI":"10.1530\/RAF-23-0024"},{"key":"ref14","doi-asserted-by":"crossref","unstructured":"Chen YJ, Liu C, Tu ZZ , Lu Q, Messerlian C, Mustieles V, et al. Associations of Urinary Trichloroacetic Acid Concentrations with Spermatozoa Apoptosis and DNA Damage in a Chinese Population. Environ Sci Technol 2022; 56(10): 6491-9;","DOI":"10.1021\/acs.est.1c07725"},{"key":"ref15","doi-asserted-by":"crossref","unstructured":"Jiang W, Zhu L, Gao L, Wang J, Fang J, Chen L, et al. What sperm parameters effect blastocyst formation and quality during ICSI with severe male infertility. Syst Biol Reprod Med 2024; 70(1): 218-27;","DOI":"10.1080\/19396368.2024.2375710"},{"key":"ref16","doi-asserted-by":"crossref","unstructured":"Liu K, Mao X , Pan F Chen Y An R. Correlation analysis of sperm DNA fragmentation index with semen parameters and the effect of sperm DFI on outcomes of ART Sci Rep 2023; 13(1): 2717;","DOI":"10.1038\/s41598-023-28765-z"},{"key":"ref17","doi-asserted-by":"crossref","unstructured":"Aghazarian A, Huf W, Pfluger H, Klatte T Standard Semen Parameters vs. Sperm Kinematics to Predict Sperm DNA Damage. World J Mens Health 2021; 39(1): 116-22;","DOI":"10.5534\/wjmh.190095"},{"key":"ref18","doi-asserted-by":"crossref","unstructured":"Ferrigno A, Ruvolo G, Capra G, Serra N, Bosco L. Correlation between the DNA fragmentation index (DFI) and sperm morphology of infertile patients. J Assist Reprod Genet 2021; 38(4): 979-86;","DOI":"10.1007\/s10815-021-02080-w"},{"key":"ref19","doi-asserted-by":"crossref","unstructured":"Sallam HN, Gelbaya TA, Maldonado Rosas I, Anagnostopoulou C, Sallam N, Agarwal A. Clinical aspects of oocyte retrieval and embryo transfer: tips and tricks for the novice and the expert. Panminerva Med 2022; 64(2): 185-99;","DOI":"10.23736\/S0031-0808.22.04679-1"},{"key":"ref20","doi-asserted-by":"crossref","unstructured":"Chiti MC, Donnez J, Amorim CA, Dolmans MM. From isolation of human ovarian follicles to the artificial ovary: tips and tricks. Minerva Ginecol 2018; 70(4): 444-55;","DOI":"10.23736\/S0026-4784.18.04231-4"}],"container-title":["Journal of Medical Biochemistry"],"original-title":["Dijagnosti\u010dka ta\u010dnost indeksa fragmentacije DNK sperme kod neplodnosti mu\u0161karaca - kohortna studija"],"language":"en","link":[{"URL":"https:\/\/scindeks-clanci.ceon.rs\/data\/pdf\/1452-8258\/2025\/1452-82582509976Z.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T12:52:15Z","timestamp":1767703935000},"score":1,"resource":{"primary":{"URL":"https:\/\/scindeks.ceon.rs\/Article.aspx?artid=1452-82582509976Z"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025]]},"references-count":20,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2025]]}},"URL":"https:\/\/doi.org\/10.5937\/jomb0-59605","relation":{},"ISSN":["1452-8258","1452-8266"],"issn-type":[{"type":"print","value":"1452-8258"},{"type":"electronic","value":"1452-8266"}],"subject":[],"published":{"date-parts":[[2025]]}}}