{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T01:29:59Z","timestamp":1773797399877,"version":"3.50.1"},"reference-count":21,"publisher":"Centre for Evaluation in Education and Science (CEON\/CEES)","issue":"3","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/BY\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Med Biochemistry"],"published-print":{"date-parts":[[2026]]},"abstract":"<jats:p>Background: This study aims to explore the regulatory effects of platelet-rich fibrin (PRF) on serum inflammatory response, bone metabolic balance and cell differentiation ability in patients with temporomandibular joint osteoarthritis (TMJ-OA) at the molecular level. Methods: Sixty patients were randomly divided into Group A and Group B (30 cases in each group). Group A was treated with meloxicam dispersible combined with hydrox-ychloroquine sulfate (HCQ); Group B was supplemented with autologous PRF intra-articular injection on the basis of Group A. Fasting venous blood was collected before treat-ment and 3 months after treatment. Inflammatory and bone metabolism markers such as IL-ip, IL-6, TNF-a, OPG, OPN, ALP and Runx2 were detected by e L iSA, and coagulation indicators such as A PTT and PT were deter-mined by an automatic coagulation analyzer. Results: After treatment, the inflammatory factors (IL-ip, IL-6, TNF-a) in both groups decreased significantly, but the decrease in group B was more significant (P&amp;lt; 0.05). Bone metabolism indicators showed that the improvement in group B was significantly better than that in group A (P&amp;lt; 0.05). Among the cell differentiation-related factors, the expression levels of Runx2, TGF-pi and Sox9 in group B were significantly increased compared with those before treatment (P&amp;lt; 0.05), and were higher than those in group A (P&amp;lt; 0.05). Conclusions: The use of PRF can optimize the therapeutic effect of TMJ-OA, improve inflammatory responses, promote cell differentiation and improve coagulation function parameters.<\/jats:p>","DOI":"10.5937\/jomb0-60052","type":"journal-article","created":{"date-parts":[[2025,8,26]],"date-time":"2025-08-26T08:01:37Z","timestamp":1756195297000},"page":"586-593","source":"Crossref","is-referenced-by-count":0,"title":["The influence of platelet-rich fibrin combined with hydroxychloroquine on the inflammatory response; bone metabolism; and cell differentiation ability in temporomandibular joint osteoarthritis"],"prefix":"10.5937","volume":"45","author":[{"given":"Yuhan","family":"Zhao","sequence":"first","affiliation":[{"name":"Chongqing Medical University, College of Stomatology, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongwei","family":"Dai","sequence":"additional","affiliation":[{"name":"Chongqing Medical University, College of Stomatology, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"3964","reference":[{"key":"ref1","doi-asserted-by":"crossref","unstructured":"Stoustrup P Twilt M, Herlin T Systemic Treatment for Temporomandibular Joint Arthritis in Juvenile Idiopathic Arthritis. J Rheumatol 2020; 47(6): 793-5;","DOI":"10.3899\/jrheum.191169"},{"key":"ref2","doi-asserted-by":"crossref","unstructured":"Ayyildiz V, Aydin Y, Ogul H. Aspergilloma secondary to pulmonary tuberculosis and tuberculous temporomandibular joint arthritis. Br J Hosp Med (Lond) 2020; 81(11): 1;","DOI":"10.12968\/hmed.2020.0261"},{"key":"ref3","doi-asserted-by":"crossref","unstructured":"Hechler BL, Shaun Matthews N. Re: The role of alloplastic reconstruction of the temporomandibular joint in the juvenile idiopathic arthritis population. Br J Oral Maxillofac Surg 2022; 60(5): 670-1;","DOI":"10.1016\/j.bjoms.2021.12.004"},{"key":"ref4","doi-asserted-by":"crossref","unstructured":"Hechler BL, Matthews NS. Role of alloplastic reconstruction of the temporomandibular joint in the juvenile idiopathic arthritis population. Br J Oral Maxillofac Surg 2021; 59(1): 21-7;","DOI":"10.1016\/j.bjoms.2020.06.039"},{"key":"ref5","doi-asserted-by":"crossref","unstructured":"Worede F Alsaikhan N, Chang CP Granquist EJ, Cahill AM. Preoperative Arterial Coil Embolization for Temporomandibular Joint (TMJ) Surgery in Children with TMJ Ankylosis. J Craniofac Surg 2022; 33(7): 2001-4;","DOI":"10.1097\/SCS.0000000000008629"},{"key":"ref6","doi-asserted-by":"crossref","unstructured":"Egierska D, Perszke M, Mazur M, Dus-Ilnicka I. Platelet-rich plasma and platelet-rich fibrin in oral surgery: A narrative review. Dent Med Probl 2023; 60(1): 177-86;","DOI":"10.17219\/dmp\/147298"},{"key":"ref7","doi-asserted-by":"crossref","unstructured":"Song P He D, Ren S, Fan L, Sun J. Platelet-rich fibrin in dentistry. J Appl Biomater Funct Mater 2024; 22: 22808000241299588;","DOI":"10.1177\/22808000241299588"},{"key":"ref8","doi-asserted-by":"crossref","unstructured":"Kargarpour Z, Nasirzade J, Panahipour L, Miron RJ, Gruber R. Platelet-Rich Fibrin Decreases the Inflammatory Response of Mesenchymal Cells. Int J Mol Sci 2021; 22(21);","DOI":"10.3390\/ijms222111333"},{"key":"ref9","doi-asserted-by":"crossref","unstructured":"Mohammadi S. Innate immunity and wound repair: The platelet-rich fibrin advantage. World J Biol Chem 2025; 16(2): 107195;","DOI":"10.4331\/wjbc.v16.i2.107195"},{"key":"ref10","doi-asserted-by":"crossref","unstructured":"Schiffman E, Ohrbach R, Truelove E, Look J, Anderson G, Goulet JP, List T, Svensson P , Gonzalez Y, Lobbezoo F , Michelotti A, Brooks SL, Ceusters W, Drangsholt M, Ettlin D, Gaul C, Goldberg LJ, Haythornthwaite JA, Hollender L, Jensen R, John MT, De Laat A, de Leeuw R, Maixner W, van der Meulen M, Murray GM, Nixdorf DR, Palla S, Petersson A, Pionchon P Smith B, Visscher CM, Zakrzewska J, Dworkin SF, International Rdc\/Tmd Consortium Network IafDR, Orofacial Pain Special Interest Group IAftSoP Diagnostic Criteria for Temporomandibular Disorders (DC\/TM D) for Clinical and Research Applications: recommendations of the International RDC\/TMD Consortium Network* and Orofacial Pain Special Interest Groupdagger. J Oral Facial Pain Headache 2014; 28(1): 6-27;","DOI":"10.11607\/jop.1151"},{"key":"ref11","doi-asserted-by":"crossref","unstructured":"Lu K, Ma F Yi D, Yu H, Tong L, Chen D. Molecular signaling in temporomandibular joint osteoarthritis. J Orthop Translat 2022; 32: 21-7;","DOI":"10.1016\/j.jot.2021.07.001"},{"key":"ref12","doi-asserted-by":"crossref","unstructured":"Coucke B, Dilissen E, Cremer J, Schrijvers R, Theys T Van Gerven L. Leukocyte-and Platelet-Rich Fibrin for enhanced tissue repair: an in vitro study characterizing cellular composition, growth factor kinetics and transcriptomic insights. Mol Biol Rep 2024; 51(1): 954;","DOI":"10.1007\/s11033-024-09890-y"},{"key":"ref13","doi-asserted-by":"crossref","unstructured":"In 't Veld AE, Jansen MAA, Ciere LCA, Moerland M. Hydroxychloroquine Effects on TLR Signalling: Underexposed but Unneglectable in COVID-19. J Immunol Res 2021; 2021: 6659410;","DOI":"10.1155\/2021\/9789246"},{"key":"ref14","doi-asserted-by":"crossref","unstructured":"Cafferata EA, Monasterio G, Castillo F , Carvajal P Flores G, Diaz W, Fuentes AD, Vernal R. Overexpression of MMPs, cytokines, and RANKL\/OPG in temporomandibular joint osteoarthritis and their association with joint pain, mouth opening, and bone degeneration: A preliminary report. Oral Dis 2021; 27(4): 970-80;","DOI":"10.1111\/odi.13623"},{"key":"ref15","doi-asserted-by":"crossref","unstructured":"Zheng S, Zhang X , Zhao Q, Chai J, Zhang Y Liquid platelet-rich fibrin promotes the regenerative potential of human periodontal ligament cells. Oral Dis 2020; 26(8): 1755-63;","DOI":"10.1111\/odi.13501"},{"key":"ref16","doi-asserted-by":"crossref","unstructured":"Qiu W, Han X , Yu T, Jiang L, Wang X , Feng R, Duan X, Teng Y Yin H, Bokarewa MI, Deng GM. Inhibitory effect of hydroxychloroquine on glucocorticoid-induced osteoporosis in lupus therapy. Clin Transl Immunology 2024; 13(10): e70010;","DOI":"10.1002\/cti2.70010"},{"key":"ref17","doi-asserted-by":"crossref","unstructured":"Bi R, Li Q, Li H, Wang P Fang H, Yang X , Wang Y, Hou Y, Ying B, Zhu S. Divergent chondro\/osteogenic transduction laws of fibrocartilage stem cell drive temporomandibular joint osteoarthritis in growing mice. Int J Oral Sci 2023; 15(1): 36;","DOI":"10.1038\/s41368-023-00240-5"},{"key":"ref18","doi-asserted-by":"crossref","unstructured":"Cheng B, Feng F Shi F Huang J, Zhang S, Quan Y Tu T, Liu Y, Wang J, Zhao Y Zhang M. Distinctive Roles of Wnt Signaling in Chondrogenic Differentiation of BMSCs under Coupling of Pressure and Platelet-Rich Fibrin. Tissue Eng Regen Med. 2022; 19(4): 823-37;","DOI":"10.1007\/s13770-022-00456-2"},{"key":"ref19","doi-asserted-by":"crossref","unstructured":"Fujii Y, Yoshida T Sato A, Ikehata M, Hatori A, Chikazu D, Ghanaati S, Kawase-Koga Y Platelet-rich fibrin-conditioned medium promotes osteogenesis of dental pulp stem cells through TGF-beta and PDGF signaling. Regen Ther 2025; 30: 100-6;","DOI":"10.1016\/j.reth.2025.05.006"},{"key":"ref20","doi-asserted-by":"crossref","unstructured":"Huang Z, Cai Y, Yang Y, Shi J, Zhao X , Mo H, Zeng Q. Effects of ultrasound-guided lumbar-sciatic nerve block and epidural anesthesia on the levels of IL-6, IL-8, TNF-alpha and coagulation factors in peripheral blood of elderly patients after hip arthroplasty. J Med Biochem 2022; 41(4): 433-40;","DOI":"10.5937\/jomb0-35847"},{"key":"ref21","doi-asserted-by":"crossref","unstructured":"Kovalenko TA, Giraud MN, Eckly A, Ribba AS, Proamer F Fraboulet S, Podoplelova NA, Valentin J, Panteleev MA, Gonelle-Gispert C, Cook S, Lafanechere L, Sveshnikova AN, Sadoul K. Asymmetrical Forces Dictate the Distribution and Morphology of Platelets in Blood Clots. Cells 2021; 10(3);","DOI":"10.3390\/cells10030584"}],"container-title":["Journal of Medical Biochemistry"],"original-title":["Uticaj fibrina bogatog trombocitima u kombinaciji sa hidroksihlorokinom na inflamatorni odgovor; metabolizam kostiju; i sposobnost \u0107elijske diferencijacije kod osteoartritisa temporomandibularnog zgloba"],"language":"en","link":[{"URL":"https:\/\/aseestant.ceon.rs\/index.php\/jomb\/article\/download\/60052\/28540","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/scindeks-clanci.ceon.rs\/data\/pdf\/1452-8258\/2026\/1452-82582603586Z.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T21:16:48Z","timestamp":1773782208000},"score":1,"resource":{"primary":{"URL":"https:\/\/scindeks.ceon.rs\/Article.aspx?artid=1452-82582603586Z"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"references-count":21,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026]]}},"URL":"https:\/\/doi.org\/10.5937\/jomb0-60052","relation":{},"ISSN":["1452-8258","1452-8266"],"issn-type":[{"value":"1452-8258","type":"print"},{"value":"1452-8266","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]}}}