{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T21:14:28Z","timestamp":1772226868340,"version":"3.50.1"},"reference-count":34,"publisher":"Centre for Evaluation in Education and Science (CEON\/CEES)","issue":"2","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/BY\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Med Biochemistry"],"published-print":{"date-parts":[[2026]]},"abstract":"<jats:p>Background: Vitamin B12 is an essential biomolecule involved in DNA synthesis, epigenetic regulation, and energy metabolism. Maternal vitamin B12 deficiency during pregnancy has been linked to oxidative stress and impaired DNA integrity. This study aimed to investigate oxidative stress and DNA damage biomarkers in term newborns with vitamin B12 deficiency and to assess their diagnostic value. Methods: A single-centre, prospective case-control study was conducted in 48 term newborns with vitamin B12 deficiency and 40 matched healthy controls. Cord venous blood samples were analysed for vitamin B12, oxidative stress parameters, and a DNA damage marker. Diagnostic performance was evaluated by receiver operating characteristic analysis. Results: Vitamin B12 levels were markedly lower in the patient group than in controls. Newborns with vitamin B12 deficiency had significantly higher levels of 8-hydroxy-2'-deoxyguanosine, total oxidant status, and oxidative stress index, whereas total antioxidant status did not differ. Receiver operating characteristic analysis demonstrated the highest diagnostic accuracy for total oxidant status, followed by 8-hydroxy-2'-deoxyguanosine and oxidative stress index. Conclusions: Oxidative stress and DNA damage were clearly increased in vitamin B12-deficient newborns. These findings suggest that maternal vitamin B12 deficiency leaves detectable biochemical imprints at birth. This prospective analysis highlights that total oxidant status, oxidative stress index, and 8-hydroxy-2'-deoxyguanosine may serve as valuable diagnostic biomarkers and, to our knowledge, represents one of the few studies linking vitamin B12 deficiency to oxidative damage in newborns.<\/jats:p>","DOI":"10.5937\/jomb0-60757","type":"journal-article","created":{"date-parts":[[2025,11,25]],"date-time":"2025-11-25T08:30:21Z","timestamp":1764059421000},"page":"376-385","source":"Crossref","is-referenced-by-count":0,"title":["Oxidative stress and DNA damage profile in cord blood of vitamin B12-deficient newborns"],"prefix":"10.5937","volume":"45","author":[{"given":"Dogan","family":"Kose","sequence":"first","affiliation":[{"name":"Harran University, Faculty of Medicine, Department of Paediatric Haematology and Oncology, Sanliurfa, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahmut","family":"Demir","sequence":"additional","affiliation":[{"name":"Harran University, Faculty of Medicine, Department of Paediatrics, Sanliurfa, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adnan","family":"Kirmit","sequence":"additional","affiliation":[{"name":"Harran University, Faculty of Medicine, Department of Biochemistry, Sanliurfa, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"3964","reference":[{"key":"ref1","doi-asserted-by":"crossref","unstructured":"Vickers MH. Early life nutrition, epigenetics and programming of later life disease. Nutrients 2014; 6: 2165-78;","DOI":"10.3390\/nu6062165"},{"key":"ref2","doi-asserted-by":"crossref","unstructured":"La Colla A, Camara CA, Campisano S, Chisari AN. Mitochondrial dysfunction and epigenetics underlying the link between early-life nutrition and non-alcoholic fatty liver disease. Nutr Res Rev 2023; 36: 281-94;","DOI":"10.1017\/S0954422422000038"},{"key":"ref3","doi-asserted-by":"crossref","unstructured":"Finkelstein JL, Layden AJ, Stover PJ. Vitamin B12 and perinatal health. Adv Nutr 2015; 6: 552-63;","DOI":"10.3945\/an.115.008201"},{"key":"ref4","doi-asserted-by":"crossref","unstructured":"Wirthensohn M, Wehrli S, Ljungblad UW, Huemer M. Biochemical, nutritional, and clinical parameters of vitamin B12 deficiency in infants: a systematic review and analysis of 292 cases published between 1962 and 2022. Nutrients 2023; 15: 4960;","DOI":"10.3390\/nu15234960"},{"key":"ref5","doi-asserted-by":"crossref","unstructured":"Tat L, Cannizzaro N, Schaaf Z, Racherla S, Bottiglieri T, Green R, et al. Prenatal folic acid and vitamin B12 imbalance alter neuronal morphology and synaptic density in the mouse neocortex. Commun Biol 2023; 6: 1133;","DOI":"10.1038\/s42003-023-05492-9"},{"key":"ref6","doi-asserted-by":"crossref","unstructured":"O'Leary F, Samman S. Vitamin B12 in health and disease. Nutrients 2010; 2: 299-316;","DOI":"10.3390\/nu2030299"},{"key":"ref7","doi-asserted-by":"crossref","unstructured":"van de Lagemaat EE, de Groot LCPGM, van den Heuvel EGHM. Vitamin B12 in relation to oxidative stress: a systematic review. Nutrients 2019; 11: 482;","DOI":"10.3390\/nu11020482"},{"key":"ref8","doi-asserted-by":"crossref","unstructured":"Cadet J, Wagner JR. DNA base damage by reactive oxygen species, oxidising agents, and UV radiation. Cold Spring Harb Perspect Biol 2013; 5: a012559;","DOI":"10.1101\/cshperspect.a012559"},{"key":"ref9","doi-asserted-by":"crossref","unstructured":"Tain YL, Hsu CN. Perinatal oxidative stress and kidney health: bridging the gap between animal models and clinical reality. Antioxidants (Basel) 2022; 12: 13;","DOI":"10.3390\/antiox12010013"},{"key":"ref10","doi-asserted-by":"crossref","unstructured":"Siddiqua TJ, Akhtar E, Haq MA, Shahab-Ferdows S, Hampel D, Islam S, et al. Effects of vitamin B12 supplementation on oxidative stress markers and proinflammatory cytokines during pregnancy and postpartum among Bangladeshi mother-child pairs. BMC Nutr 2024; 10: 3;","DOI":"10.1186\/s40795-023-00785-y"},{"key":"ref11","doi-asserted-by":"crossref","unstructured":"Erel O. A new automated colorimetric method for measuring total oxidant status. Clin Biochem 2005; 38: 1103-11;","DOI":"10.1016\/j.clinbiochem.2005.08.008"},{"key":"ref12","doi-asserted-by":"crossref","unstructured":"Erel O. A novel automated method to measure total antioxidant response against potent free radical reactions. Clin Biochem 2004; 37: 112-19;","DOI":"10.1016\/j.clinbiochem.2003.10.014"},{"key":"ref13","unstructured":"Cayman Chemical. DNA\/RNA Oxidative Damage (High Sensitivity) ELISA Kit - Item No. 589320 [Internet]. Ann Arbor (MI): Cayman Chemical; [cited 2025 Aug 2]. Available from: https:\/\/www.caymanchem.com\/product\/589320;"},{"key":"ref14","doi-asserted-by":"crossref","unstructured":"Friso S, Choi SW. Gene-nutrient interactions in one-carbon metabolism. Curr Drug Metab 2005; 6: 37-46;","DOI":"10.2174\/1389200052997339"},{"key":"ref15","doi-asserted-by":"crossref","unstructured":"Valavanidis A, Vlachogianni T, Fiotakis K. 8-hydroxy-2'-deoxyguanosine (8-OHdG): A critical biomarker of oxidative stress and carcinogenesis. J Environ Sci Health C Environ Carcinog Ecotoxicol Rev 2009; 27: 120-39;","DOI":"10.1080\/10590500902885684"},{"key":"ref16","doi-asserted-by":"crossref","unstructured":"Shigenaga MK, Ames BN. Assays for 8-hydroxy-2'-deoxyguanosine: a biomarker of in vivo oxidative DNA damage. Free Radic Biol Med 1991; 10: 211-16;","DOI":"10.1016\/0891-5849(91)90078-H"},{"key":"ref17","doi-asserted-by":"crossref","unstructured":"Elkabany ZA, El-Farrash RA, Shinkar DM, Ismail EA, Nada AS, Farag AS, et al. Oxidative stress markers in neonatal respiratory distress syndrome: advanced oxidation protein products and 8-hydroxy-2-deoxyguanosine in relation to disease severity. Pediatr Res 2020; 87: 74-80;","DOI":"10.1038\/s41390-019-0464-y"},{"key":"ref18","doi-asserted-by":"crossref","unstructured":"Shoji H, Ikeda N, Hosozawa M, Ohkawa N, Matsunaga N, Suganuma H, et al. Oxidative stress early in infancy and neurodevelopmental outcome in very low-birth-weight infants. Pediatr Int 2014; 56: 709-13;","DOI":"10.1111\/ped.12332"},{"key":"ref19","doi-asserted-by":"crossref","unstructured":"Firat CK, Ozkan BN, G\u00fcler EM. Beneficial effects of vitamin B12 treatment in pediatric patients diagnosed with vitamin B12 deficiency regarding total-native thiol, oxidative stress, and mononuclear leukocyte DNA damage. Free Radic Res 2022; 56: 631-39;","DOI":"10.1080\/10715762.2022.2162392"},{"key":"ref20","doi-asserted-by":"crossref","unstructured":"Lembo C, Buonocore G, Perrone S. Oxidative stress in preterm newborns. Antioxidants (Basel) 2021; 10: 1672;","DOI":"10.3390\/antiox10111672"},{"key":"ref21","doi-asserted-by":"crossref","unstructured":"G\u00fcney T, Ali\u00e7ik M, Akinci S, Ne\u00e7elioglu S, Dilek i, Erel O. Evaluation of oxidant and antioxidant status in patients with vitamin B12 deficiency. Turk J Med Sci 2015; 45: 1280-84;","DOI":"10.3906\/sag-1407-81"},{"key":"ref22","doi-asserted-by":"crossref","unstructured":"Sukumar N, Rafnsson SB, Kandala NB, Bhopal R, Yajnik CS, Saravanan P. Prevalence of vitamin B-12 insufficiency during pregnancy and its effect on offspring birth weight: a systematic review and meta-analysis. Am J Clin Nutr 2016; 103: 1232-51;","DOI":"10.3945\/ajcn.115.123083"},{"key":"ref23","doi-asserted-by":"crossref","unstructured":"Rogne T, Tielemans MJ, Chong MF-F, Yajnik CS, Krishnaveni GV, Poston L, et al. Associations of maternal vitamin B12 concentration in pregnancy with the risks of preterm birth and low birth weight: a systematic review and meta-analysis of individual participant data. Am J Epidemiol 2017; 185: 212-23;","DOI":"10.1093\/aje\/kww212"},{"key":"ref24","doi-asserted-by":"crossref","unstructured":"Roy S, Kale A, Dangat K, Sable P, Kulkarni A, Joshi S. Maternal micronutrients (folic acid and vitamin B12) and omega-3 fatty acids: implications for neurodevelopmental risk in the rat offspring. Brain Dev 2012; 34: 64-71;","DOI":"10.1016\/j.braindev.2011.01.002"},{"key":"ref25","doi-asserted-by":"crossref","unstructured":"McCullough LE, Miller EE, Mendez MA, Murtha AP, Murphy SK, Hoyo C. Maternal B vitamins: effects on offspring weight and DNA methylation at genomically imprinted domains. Clin Epigenetics 2016; 8: 8;","DOI":"10.1186\/s13148-016-0174-9"},{"key":"ref26","doi-asserted-by":"crossref","unstructured":"Monasso GS, Hoang TT, Mancano G, Fernandez-Barr\u00e9s S, Dou J, Jaddoe VWV, et al. A meta-analysis of epigenome-wide association studies on pregnancy vitamin B12 concentrations and offspring DNA methylation. Epigenetics 2023; 18: 2202835;","DOI":"10.1080\/15592294.2023.2202835"},{"key":"ref27","doi-asserted-by":"crossref","unstructured":"Dfaz-Garc\u00eda H, Vilchis-Gil J, Garc\u00eda-Roca P, Kl\u00fcnder-Kl\u00fcnder M, Gomez-Lopez J, Granados-Riveron JT, et al. Dietary and antioxidant vitamins limit the DNA damage mediated by oxidative stress in the mother-newborn binomial. Life (Basel) 2022; 12: 1012;","DOI":"10.3390\/life12071012"},{"key":"ref28","doi-asserted-by":"crossref","unstructured":"Atas N, Cakmak A. The Frequency of Vitamin B12 and Folic Acid Deficiency in Mothers and Their Newborn Infants in anliurfa Province (in Turkish). Harran Univ Med Fac J 2023; 20: 149-54;","DOI":"10.35440\/hutfd.1254961"},{"key":"ref29","doi-asserted-by":"crossref","unstructured":"Koc A, Kocyigit A, Soran M, Demir N, Sevinc E, Erel O, et al. High frequency of maternal vitamin B12 deficiency as an important cause of infantile vitamin B12 deficiency in Sanliurfa province of Turkey. Eur J Nutr 2006; 45: 291-7;","DOI":"10.1007\/s00394-006-0598-7"},{"key":"ref30","doi-asserted-by":"crossref","unstructured":"Zych B, Gorka A, Myszka A, Btoniarz D, Siekierzy ska A, Bta W. Status of oxidative stress during low-risk labour: preliminary data. Int J Environ Res Public Health 2023; 20: 157;","DOI":"10.3390\/ijerph20010157"},{"key":"ref31","doi-asserted-by":"crossref","unstructured":"Ibrahim A, Khoo MI, Ismail EHE, Hussain NHN, Zin AAM, Noordin L, et al. Oxidative stress biomarkers in pregnancy: a systematic review. Reprod Biol Endocrinol 2024; 22: 93;","DOI":"10.1186\/s12958-024-01259-x"},{"key":"ref32","doi-asserted-by":"crossref","unstructured":"Drejza MA, Rylewicz K, Majcherek E, Gross-Tyrkin K, Mizgier M, Plagens-Rotman K, et al. Markers of oxidative stress in obstetrics and gynaecology - a systematic literature review. Antioxidants (Basel) 2022; 11: 1477;","DOI":"10.3390\/antiox11081477"},{"key":"ref33","doi-asserted-by":"crossref","unstructured":"Guo Q, Jin Y, Chen X, Y X, Shen X, Lin M, et al. NF-k B in biology and targeted therapy: new insights and translational implications. Signal Transduct Target Ther 2024; 9: 53;","DOI":"10.1038\/s41392-024-01757-9"},{"key":"ref34","unstructured":"Ali AAH, Mohamed FHA, Hago S, Elgali IFES, Mohammed HES, Mirghani RG. The neurological sequelae of vitamin B12 deficiency: A systematic review and randomised controlled trial. Cureus 2025; 17: e83668;"}],"container-title":["Journal of Medical Biochemistry"],"original-title":["Oksidativni stres i profil o\u0161te\u0107enja DNK u pup\u010danoj krvi kod novoro\u0111en\u010dadi sa deficitom vitamina B12"],"language":"en","link":[{"URL":"https:\/\/scindeks-clanci.ceon.rs\/data\/pdf\/1452-8258\/2026\/1452-82582602376K.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T20:43:23Z","timestamp":1772225003000},"score":1,"resource":{"primary":{"URL":"https:\/\/scindeks.ceon.rs\/Article.aspx?artid=1452-82582602376K"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"references-count":34,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026]]}},"URL":"https:\/\/doi.org\/10.5937\/jomb0-60757","relation":{},"ISSN":["1452-8258","1452-8266"],"issn-type":[{"value":"1452-8258","type":"print"},{"value":"1452-8266","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]}}}