{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T15:21:41Z","timestamp":1785424901358,"version":"3.56.0"},"reference-count":15,"publisher":"Centre for Evaluation in Education and Science (CEON\/CEES)","issue":"6","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/BY\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Med Biochemistry"],"published-print":{"date-parts":[[2026]]},"abstract":"<jats:p>Background: Low-density lipoproteins cholesterol (LDL-C) is a key parameter for assessing the risk of atherosclerotic cardiovascular disease. Direct measurement of LDL-C is not always possible due to practical or financial reasons, making the use of calculation formulas essential. The aim of this study was to evaluate the applicability of four different formulas proposed by Friedewald, Sampson, Anandaraja, and Martin-Hopkins for calculating LDL-C compared to direct method in patients with serum triglyceride levels from 4.5 to 9.0 mmol\/L in the population of Vojvodina. Methods: The retrospective study included 272 subjects whose lipid status parameters were measured between June 2022 and June 2023. LDL-C was determined by the direct method (d-LDL-C) on the Alinity c analyser (Abbott Laboratories, Illinois, USA). Calculated LDL-C values were obtained using the four selected formulas. Results: The average age of participants was 52 years, and the median concentration of triglycerides was 5.48 (4.94-6.58) mmol\/L. A statistically significant positive correlation was found between d-LDL-C and all calculated LDL-C (P&amp;lt; 0.001). Linear regression analysis of the data showed the best agreement between d-LDL-C and the Sampson formula (slope=0.97; 95% C I=0.91 - 1.04). The smallest mean difference (MD) was observed between d-LDL-C and the Sampson formula (M D=-0.032). When comparing d-LDL-C and LDL-C values obtained by calculation, only the formula of Sampson et al. did not show a statistically significant difference (P= 0.240). Conclusion: In the studied population of patients with hypertriglyceridemia, the Sampson et al. formula showed the best performance compared with the others tested.<\/jats:p>","DOI":"10.5937\/jomb0-61948","type":"journal-article","created":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T08:57:16Z","timestamp":1770281836000},"page":"1354-1363","source":"Crossref","is-referenced-by-count":0,"title":["Applicability of LDL-cholesterol calculation formulas in hypertriglyceridemia: Insights from the Vojvodina"],"prefix":"10.5937","volume":"45","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0114-2989","authenticated-orcid":false,"given":"Dragana","family":"\u017duvi\u0107","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00fpn0e94","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7859-0923","authenticated-orcid":false,"given":"Stanislava","family":"Nikoli\u0107","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00fpn0e94","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4034-1585","authenticated-orcid":false,"given":"Romana","family":"Mijovi\u0107","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00fpn0e94","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6160-8173","authenticated-orcid":false,"given":"Branislava","family":"Ilin\u010di\u0107","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00fpn0e94","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aneta","family":"Ran\u0111elovi\u0107-\u017divkovi\u0107","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00fpn0e94","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-2075-4754","authenticated-orcid":false,"given":"Du\u0161an","family":"Sedlarevi\u0107","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00fpn0e94","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7166-3379","authenticated-orcid":false,"given":"Velibor","family":"\u010cabarkapa","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00fpn0e94","id-type":"ROR","asserted-by":"publisher"}]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"3964","reference":[{"key":"ref1","doi-asserted-by":"crossref","unstructured":"\u0110eri\u0107 M, Koji\u0107-Damjanov S, Cabarkapa V, Eremi\u0107 N. Biochemical markers of atherosclerosis. J Med Biochem 2008; 27(2): 148-53;","DOI":"10.2478\/v10011-008-0008-1"},{"key":"ref2","doi-asserted-by":"crossref","unstructured":"Mach F, Baigent C, Catapano AL, Koskinas KC, Casula M, Badimon L, et al. 2019 ESC\/EAS guidelines for the management of dyslipidaemias: lipid modification to reduce cardiovascular risk. Eur Heart J 2020; 41(1): 111-88;","DOI":"10.15829\/1560-4071-2020-3826"},{"key":"ref3","doi-asserted-by":"crossref","unstructured":"Sirivelu B, Namilakonda M, Soma K, Thallapaneni S, Annavarapu DL, Kumar VS, et al. Assessing the validity of nine different formulae for LDL-C estimation in a Tertiary Care Centre, Hyderabad, India. J Clin and Diagn Res 2022; 16(1): 6-11;","DOI":"10.7860\/JCDR\/2022\/50590.15903"},{"key":"ref4","doi-asserted-by":"crossref","unstructured":"Maki K, Grant J, Orringer C. LDL-C Estimation: The perils of living with imperfection. J Am Coll Cardiol 2022; 79(6): 542-4;","DOI":"10.1016\/j.jacc.2021.12.005"},{"key":"ref5","doi-asserted-by":"crossref","unstructured":"Martins J, Steyn N, Rossouw HM, Pillay TS. Best practice for LDL-cholesterol: when and how to calculate. J Clin Pathol 2023; 76: 145-52;","DOI":"10.1136\/jcp-2022-208480"},{"key":"ref6","doi-asserted-by":"crossref","unstructured":"Piani F Cicero AFG, Ventura F Dormi A, Fogacci F Patrono D, et al. Evaluation of twelve formulas for LDL-C estimation in a large, blinded, random Italian population. Int J Cardiol 2021; 330: 221-7;","DOI":"10.1016\/j.ijcard.2021.02.009"},{"key":"ref7","unstructured":"Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 1972; 18(6): 499-502;"},{"key":"ref8","doi-asserted-by":"crossref","unstructured":"Sampson M, Ling C, Sun Q, Harb R, Ashmaig M, Warnick R, et al. 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Comparison of Friedewald, Martin\/Hopkins, and Sampson formulae with direct LDL measurement in hyperlipidaemic and normolipidaemic adults in a Turkish population. J Med Biochem 2024; 43: 671-80;","DOI":"10.5937\/jomb0-46549"},{"key":"ref15","doi-asserted-by":"crossref","unstructured":"Sampson M, Wolska A, Meeusen JW, Otvos J, Remaley AT. The Sampson-NIH equation is the preferred calculation method for LDL-C. Clin Chem 2024; 70(2): 399-402;","DOI":"10.1093\/clinchem\/hvad190"}],"container-title":["Journal of Medical Biochemistry"],"original-title":["Primenjivost formula za izra\u010dunavanje LDL-holesterola u hipertrigliceridemiji - uvidi iz Vojvodine"],"language":"en","link":[{"URL":"https:\/\/scindeks-clanci.ceon.rs\/data\/pdf\/1452-8258\/2026\/1452-82582606354Q.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T13:48:30Z","timestamp":1781617710000},"score":1,"resource":{"primary":{"URL":"https:\/\/scindeks.ceon.rs\/Article.aspx?artid=1452-82582606354Q"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"references-count":15,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026]]}},"URL":"https:\/\/doi.org\/10.5937\/jomb0-61948","relation":{},"ISSN":["1452-8258","1452-8266"],"issn-type":[{"value":"1452-8258","type":"print"},{"value":"1452-8266","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026]]}}}