{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,4]],"date-time":"2024-06-04T02:41:27Z","timestamp":1717468887919},"reference-count":45,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2013,5,21]],"date-time":"2013-05-21T00:00:00Z","timestamp":1369094400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Since induced pluripotent stem (iPS) cells have differentiation potential into all three germ layer-derived tissues, efficient purification of target cells is required in many fields of iPS research. One useful strategy is isolation of desired cells from differentiated iPS cells by lineage-specific expression of a drug-resistance gene, followed by drug selection. With this strategy, we purified neural stem\/progenitor cells (NSCs), a good candidate source for regenerative therapy, from differentiated mouse iPS cells. We constructed a bicistronic expression vector simultaneously expressing blasticidin S resistance gene and DsRed under the control of tandem enhancer of a 257-base pair region of nestin second intron, an NSC-specific enhancer. This construct was efficiently inserted into the iPS genome by piggyBac transposon-mediated gene transfer, and the established subclone was differentiated into NSCs in the presence or absence of blasticidin S. Consequently, incubation with blasticidin S led to purification of NSCs from differentiated iPS cells. Our results suggest that a lineage-specific drug selection strategy is useful for purification of NSCs from differentiated iPS cells and that this strategy can be applied for the purification of other cell types.<\/jats:p>","DOI":"10.5966\/sctm.2012-0139","type":"journal-article","created":{"date-parts":[[2013,5,22]],"date-time":"2013-05-22T04:38:43Z","timestamp":1369197523000},"page":"420-433","source":"Crossref","is-referenced-by-count":4,"title":["Lineage-Specific Purification of Neural Stem\/Progenitor Cells From Differentiated Mouse Induced Pluripotent Stem Cells"],"prefix":"10.1093","volume":"2","author":[{"given":"Masato","family":"Maruyama","sequence":"first","affiliation":[{"name":"Department of Anatomy and Brain Science, Kansai Medical University, Hirakata, Osaka, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuji","family":"Yamashita","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Brain Science, Kansai Medical University, Hirakata, Osaka, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiko","family":"Kase","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Brain Science, Kansai Medical University, Hirakata, Osaka, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"Trifonov","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Brain Science, Kansai Medical University, Hirakata, Osaka, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsuo","family":"Sugimoto","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Brain Science, Kansai Medical University, Hirakata, Osaka, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2013,5,21]]},"reference":[{"key":"2021122707320959400_ref1","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1016\/j.cell.2006.07.024","article-title":"Induction of 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