{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T03:43:53Z","timestamp":1773287033929,"version":"3.50.1"},"reference-count":40,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2014,2,3]],"date-time":"2014-02-03T00:00:00Z","timestamp":1391385600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100003130","name":"Fonds Wetenschappelijk Onderzoek Vlaanderen","doi-asserted-by":"crossref","award":["G034613N"],"award-info":[{"award-number":["G034613N"]}],"id":[{"id":"10.13039\/501100003130","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004040","name":"KU Leuven","doi-asserted-by":"publisher","award":["EIW-B4855-EF\/05\/11"],"award-info":[{"award-number":["EIW-B4855-EF\/05\/11"]}],"id":[{"id":"10.13039\/501100004040","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004040","name":"KU Leuven","doi-asserted-by":"publisher","award":["ETH-C1900-PF"],"award-info":[{"award-number":["ETH-C1900-PF"]}],"id":[{"id":"10.13039\/501100004040","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004040","name":"KU Leuven","doi-asserted-by":"publisher","award":["EME-C2161-GOA\/11\/012"],"award-info":[{"award-number":["EME-C2161-GOA\/11\/012"]}],"id":[{"id":"10.13039\/501100004040","id-type":"DOI","asserted-by":"publisher"}]},{"name":"FWO Odysseus award, FWO","award":["G.0601.07"],"award-info":[{"award-number":["G.0601.07"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Pancreatic endocrine progenitors obtained from human embryonic stem cells (hESCs) represent a promising source to develop cell-based therapies for diabetes. Although endocrine pancreas progenitor cells have been isolated from mouse pancreata on the basis of Ngn3 expression, human endocrine progenitors have not been isolated yet. As substantial differences exist between human and murine pancreas biology, we investigated whether it is possible to isolate pancreatic endocrine progenitors from differentiating hESC cultures by lineage tracing of NGN3. We targeted the 3\u2032 end of NGN3 using zinc finger nuclease-mediated homologous recombination to allow selection of NGN3eGFP+ cells without disrupting the coding sequence of the gene. Isolated NGN3eGFP+ cells express PDX1, NKX6.1, and chromogranin A and differentiate in vivo toward insulin, glucagon, and somatostatin single hormone-expressing cells but not to ductal or exocrine pancreatic cells or other endodermal, mesodermal, or ectodermal lineages. This confirms that NGN3+ cells represent pancreatic endocrine progenitors in humans. In addition, this hESC reporter line constitutes a unique tool that may aid in gaining insight into the developmental mechanisms underlying fate choices in human pancreas and in developing cell-based therapies.<\/jats:p>","DOI":"10.5966\/sctm.2013-0078","type":"journal-article","created":{"date-parts":[[2014,2,4]],"date-time":"2014-02-04T05:43:25Z","timestamp":1391492605000},"page":"489-499","source":"Crossref","is-referenced-by-count":22,"title":["Prospectively Isolated NGN3-Expressing Progenitors From Human Embryonic Stem Cells Give Rise to Pancreatic Endocrine Cells"],"prefix":"10.1093","volume":"3","author":[{"given":"Qing","family":"Cai","sequence":"first","affiliation":[{"name":"Stamcelinstituut Leuven, University Hospitals Leuven, KU Leuven, Leuven, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paola","family":"Bonfanti","sequence":"additional","affiliation":[{"name":"Department of Human Genetics, University Hospitals Leuven, KU Leuven, Leuven, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rangarajan","family":"Sambathkumar","sequence":"additional","affiliation":[{"name":"Stamcelinstituut Leuven, University Hospitals Leuven, KU Leuven, Leuven, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kim","family":"Vanuytsel","sequence":"additional","affiliation":[{"name":"Stamcelinstituut Leuven, University Hospitals Leuven, KU Leuven, Leuven, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jolien","family":"Vanhove","sequence":"additional","affiliation":[{"name":"Stamcelinstituut Leuven, University Hospitals Leuven, KU Leuven, Leuven, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Conny","family":"Gysemans","sequence":"additional","affiliation":[{"name":"Laboratory for Experimental Medicine and Endocrinology, University Hospitals Leuven, KU Leuven, Leuven, 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