{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T01:15:37Z","timestamp":1792804537553,"version":"4.5.0"},"reference-count":47,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2021,11,19]],"date-time":"2021-11-19T00:00:00Z","timestamp":1637280000000},"content-version":"vor","delay-in-days":1919,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Radiotherapy for head and neck cancer often has undesirable effects on salivary glands that lead to xerostomia or severe dry mouth, which can increase oral infections. Our goal is to engineer functional, three-dimensional (3D) salivary gland neotissue for autologous implantation to provide permanent relief. An immediate need exists to obtain autologous adult progenitor cells as the use of embryonic and induced pluripotent stem cells potentially pose serious risks such as teratogenicity and immunogenic rejection. Here, we report an expandable population of primary salivary human stem\/progenitor cells (hS\/PCs) that can be reproducibly and scalably isolated and propagated from tissue biopsies. These cells have increased expression of progenitor markers (K5, K14, MYC, ETV4, ETV5) compared with differentiation markers of the parotid gland (acinar: MIST1\/BHLHA15 and AMY1A; ductal: K19 and TFCP2L1). Isolated hS\/PCs grown in suspension formed primary and secondary spheres and could be maintained in long-term 3D hydrogel culture. When grown in a customized 3D modular hyaluronate-based hydrogel system modified with bioactive basement membrane-derived peptides, levels of progenitor markers, indices of proliferation, and viability of hS\/PCs were enhanced. When appropriate microenvironmental cues were provided in a controlled manner in 3D, such as stimulation with \u03b2-adrenergic and cholinergic agonists, hS\/PCs differentiated into an acinar-like lineage, needed for saliva production. We conclude that the stem\/progenitor potential of adult hS\/PCs isolated without antigenic sorting or clonal expansion in suspension, combined with their ability to differentiate into specialized salivary cell lineages in a human-compatible culture system, makes them ideal for use in 3D bioengineered salivary gland applications.<\/jats:p>","DOI":"10.5966\/sctm.2016-0083","type":"journal-article","created":{"date-parts":[[2016,8,19]],"date-time":"2016-08-19T02:08:40Z","timestamp":1471572520000},"page":"110-120","source":"Crossref","is-referenced-by-count":78,"title":["Primary Salivary Human Stem\/Progenitor Cells Undergo Microenvironment-Driven Acinar-Like Differentiation in Hyaluronate Hydrogel Culture"],"prefix":"10.1093","volume":"6","author":[{"given":"Padma Pradeepa","family":"Srinivasan","sequence":"first","affiliation":[{"name":"a Department of Biological Sciences, University of Delaware, Newark, Delaware, USA"},{"name":"b Center for Translational Cancer Research, Helen F. Graham Cancer Center & Research Institute, Newark, Delaware, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vaishali N.","family":"Patel","sequence":"additional","affiliation":[{"name":"c Matrix and Morphogenesis Section, National Institute of Dental and Craniofacial Research, NIH, Bethesda, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuang","family":"Liu","sequence":"additional","affiliation":[{"name":"d Department of Materials Sciences and Engineering, University of Delaware, Newark, Delaware, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel A.","family":"Harrington","sequence":"additional","affiliation":[{"name":"e Department of BioSciences, Rice University, Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew P.","family":"Hoffman","sequence":"additional","affiliation":[{"name":"c Matrix and Morphogenesis Section, National Institute of Dental and Craniofacial Research, NIH, Bethesda, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinqiao","family":"Jia","sequence":"additional","affiliation":[{"name":"a Department of Biological Sciences, University of Delaware, Newark, Delaware, USA"},{"name":"d Department of Materials Sciences and Engineering, University of Delaware, Newark, Delaware, USA"},{"name":"f Department of Biomedical Engineering, University of Delaware, Newark, Delaware, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert L.","family":"Witt","sequence":"additional","affiliation":[{"name":"a Department of Biological Sciences, University of Delaware, Newark, Delaware, USA"},{"name":"b Center for Translational Cancer Research, Helen F. Graham Cancer Center & Research Institute, Newark, Delaware, USA"},{"name":"g Department of Otolaryngology\u2013Head & Neck Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary C.","family":"Farach-Carson","sequence":"additional","affiliation":[{"name":"a Department of Biological Sciences, University of Delaware, Newark, Delaware, USA"},{"name":"e Department of BioSciences, Rice University, Houston, Texas, USA"},{"name":"h Department of Bioengineering, Rice University, Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Swati","family":"Pradhan-Bhatt","sequence":"additional","affiliation":[{"name":"a Department of Biological Sciences, University of Delaware, Newark, Delaware, USA"},{"name":"b Center for Translational Cancer Research, Helen F. Graham Cancer Center & Research Institute, Newark, Delaware, USA"},{"name":"f Department of Biomedical Engineering, University of Delaware, Newark, Delaware, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2016,8,18]]},"reference":[{"key":"2022022809205335500_R1","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1177\/154411130301400305","article-title":"Oral sequelae of head and neck radiotherapy","volume":"14","author":"Vissink","year":"2003","journal-title":"Crit Rev Oral Biol Med"},{"key":"2022022809205335500_R2","author":"Witt","year":"2005"},{"key":"2022022809205335500_R3","doi-asserted-by":"crossref","first-page":"1804","DOI":"10.1158\/1078-0432.CCR-05-2381","article-title":"Mobilization of bone marrow stem cells by granulocyte colony-stimulating factor ameliorates radiation-induced damage to salivary glands","volume":"12","author":"Lombaert","year":"2006","journal-title":"Clin Cancer 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