{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T03:14:10Z","timestamp":1774581250695,"version":"3.50.1"},"reference-count":64,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2016,3,29]],"date-time":"2016-03-29T00:00:00Z","timestamp":1459209600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"European Union Project Cardio Repair European Multidisciplinary Initiative","award":["HEALTH-2009_242038"],"award-info":[{"award-number":["HEALTH-2009_242038"]}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["MITPTB\/ECE\/0013\/2013"],"award-info":[{"award-number":["MITPTB\/ECE\/0013\/2013"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["SFRH\/BD\/51573\/2011"],"award-info":[{"award-number":["SFRH\/BD\/51573\/2011"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"name":"CardioRegen","award":["HMSP-ICT\/0039\/2013"],"award-info":[{"award-number":["HMSP-ICT\/0039\/2013"]}]},{"name":"CardioRecept","award":["PTDC\/BBB-BIO\/1414"],"award-info":[{"award-number":["PTDC\/BBB-BIO\/1414"]}]},{"name":"iNOVA4Health","award":["UID\/Multi\/04462\/2013"],"award-info":[{"award-number":["UID\/Multi\/04462\/2013"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title\/>\n                  <jats:p>To fully explore the potential of human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs), efficient methods for storage and shipment of these cells are required. Here, we evaluated the feasibility to cold store monolayers and aggregates of functional CMs obtained from different PSC lines using a fully defined clinical-compatible preservation formulation and investigated the time frame that hPSC-CMs could be subjected to hypothermic storage. We showed that two-dimensional (2D) monolayers of hPSC-CMs can be efficiently stored at 4\u00b0C for 3 days without compromising cell viability. However, cell viability decreased when the cold storage interval was extended to 7 days. We demonstrated that hPSC-CMs are more resistant to prolonged hypothermic storage-induced cell injury in three-dimensional aggregates than in 2D monolayers, showing high cell recoveries (&amp;gt;70%) after 7 days of storage. Importantly, hPSC-CMs maintained their typical (ultra)structure, gene and protein expression profile, electrophysiological profiles, and drug responsiveness.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Significance<\/jats:title>\n                  <jats:p>The applicability of human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) in the clinic\/industry is highly dependent on the development of efficient methods for worldwide shipment of these cells. This study established effective clinically compatible strategies for cold (4\u00b0C) storage of hPSC-CMs cultured as two-dimensional (2D) monolayers and three-dimensional (3D) aggregates. Cell recovery of 2D monolayers of hPSC-CMs was found to be dependent on the time of storage, and 3D cell aggregates were more resistant to prolonged cold storage than 2D monolayers. Of note, it was demonstrated that 7 days of cold storage did not affect hPSC-CM ultrastructure, phenotype, or function. This study provides important insights into the cold preservation of PSC-CMs that could be valuable in improving global commercial distribution of hPSC-CMs.<\/jats:p>\n               <\/jats:sec>","DOI":"10.5966\/sctm.2015-0238","type":"journal-article","created":{"date-parts":[[2016,3,30]],"date-time":"2016-03-30T03:08:51Z","timestamp":1459307331000},"page":"658-669","source":"Crossref","is-referenced-by-count":43,"title":["Effective Hypothermic Storage of Human Pluripotent Stem Cell-Derived Cardiomyocytes Compatible With Global Distribution of Cells for Clinical Applications and Toxicology Testing"],"prefix":"10.1093","volume":"5","author":[{"given":"Cl\u00e1udia","family":"Correia","sequence":"first","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, Oeiras, Portugal"},{"name":"iBET, Instituto de Biologia Experimental e Tecnol\u00f3gica, Oeiras, Portugal"}]},{"given":"Alexey","family":"Koshkin","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, Oeiras, Portugal"},{"name":"iBET, Instituto de Biologia Experimental e Tecnol\u00f3gica, Oeiras, Portugal"}]},{"given":"Madalena","family":"Carido","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, Oeiras, Portugal"},{"name":"iBET, Instituto de Biologia Experimental e Tecnol\u00f3gica, Oeiras, Portugal"}]},{"given":"Nuno","family":"Espinha","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, Oeiras, Portugal"},{"name":"iBET, Instituto de Biologia Experimental e Tecnol\u00f3gica, Oeiras, Portugal"}]},{"given":"Tomo","family":"\u0160ari\u0107","sequence":"additional","affiliation":[{"name":"Center for Physiology and Pathophysiology, Institute for Neurophysiology, Medical Faculty, University of Cologne, Cologne, Germany"}]},{"given":"Pedro A.","family":"Lima","sequence":"additional","affiliation":[{"name":"Nova Medical School, Universidade Nova de Lisboa, Lisboa, Portugal"}]},{"given":"Margarida","family":"Serra","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, Oeiras, Portugal"},{"name":"iBET, Instituto de Biologia Experimental e Tecnol\u00f3gica, Oeiras, Portugal"}]},{"given":"Paula M.","family":"Alves","sequence":"additional","affiliation":[{"name":"Instituto de Tecnologia Qu\u00edmica e Biol\u00f3gica Ant\u00f3nio Xavier, Universidade Nova de Lisboa, Oeiras, Portugal"},{"name":"iBET, Instituto de Biologia Experimental e Tecnol\u00f3gica, Oeiras, Portugal"}]}],"member":"286","published-online":{"date-parts":[[2016,3,29]]},"reference":[{"key":"2021122705023593000_R1","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1016\/j.pharmthera.2014.03.004","article-title":"Induced pluripotent stem cell-derived cardiomyocytes for drug development and toxicity testing","volume":"143","author":"Sinnecker","year":"2014","journal-title":"Pharmacol Ther"},{"key":"2021122705023593000_R2","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1007\/s11936-014-0319-0","article-title":"Pluripotent stem cell derived cardiomyocytes for cardiac repair","volume":"16","author":"Lundy","year":"2014","journal-title":"Curr Treat Options Cardiovasc Med"},{"key":"2021122705023593000_R3","doi-asserted-by":"crossref","first-page":"1357","DOI":"10.1007\/s10439-013-0921-4","article-title":"Efficient and scalable expansion of human pluripotent stem cells under clinically compliant settings: A view in 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