{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T08:14:16Z","timestamp":1791360856930,"version":"4.3.1"},"reference-count":25,"publisher":"Georg Thieme Verlag KG","issue":"03","license":[{"start":{"date-parts":[[2022,3,22]],"date-time":"2022-03-22T00:00:00Z","timestamp":1647907200000},"content-version":"vor","delay-in-days":690,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2020,5,15]],"date-time":"2020-05-15T00:00:00Z","timestamp":1589500800000},"content-version":"tdm","delay-in-days":14,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["e-aps.org"],"crossmark-restriction":true},"short-container-title":["Arch Plast Surg"],"published-print":{"date-parts":[[2020,5]]},"abstract":"<jats:p>\n          Background  Surgical reconstruction of chronic wounds is often infeasible due to infection, comorbidities, or poor viability of local tissues. The aim of this study was to describe the authors\u2019 technique for improving the regenerative and antimicrobial potential of a combination of modified nanofat and platelet-rich plasma (PRP) in nonhealing infected wounds.<\/jats:p><jats:p>\n          Methods  Fourteen patients met the inclusion criteria. Fat tissue was harvested from the lower abdomen following infiltration of a solution of 1,000 mL of NaCl solution, 225 mg of ropivacaine, and 1 mg of epinephrine. Aspiration was performed using a 3-mm cannula with 1-mm holes. The obtained solution was decanted and mechanically emulsified, but was not filtered. Non-activated leukocyte-rich PRP (naLR-PRP) was added to the solution before injection. Patients underwent three sessions of injection of 8-mL naLR-PRP performed at 2-week intervals.<\/jats:p><jats:p>\n          Results  Thirteen of 14 patients completed the follow-up. Complete healing was achieved in seven patients (53.8%). Four patients (30.8%) showed improvement, with a mean ulcer width reduction of 57.5%\u00b113.8%. Clinical improvements in perilesional skin quality were reported in all patients, with reduced erythema, increased thickness, and increased pliability. An overall wound depth reduction of 76.6%\u00b140.8% was found. Pain was fully alleviated in all patients who underwent re-epithelization. A mean pain reduction of 42%\u00b133.3% (as indicated by visual analog scale score) was found in non-re-epithelized patients at a 3-month follow-up.<\/jats:p><jats:p>\n          Conclusions  The discussed technique facilitated improvement of both the regenerative and the antimicrobial potential of fat grafting. It proved effective in surgically-untreatable infected chronic wounds unresponsive to conventional therapies.<\/jats:p>","DOI":"10.5999\/aps.2019.01571","type":"journal-article","created":{"date-parts":[[2020,5,18]],"date-time":"2020-05-18T23:57:15Z","timestamp":1589846235000},"page":"217-222","update-policy":"https:\/\/doi.org\/10.5999\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Use of platelet-rich plasma and modified nanofat grafting in infected ulcers: Technical refinements to improve regenerative and antimicrobial potential"],"prefix":"10.1055","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1573-1098","authenticated-orcid":false,"given":"Francesco","family":"Segreto","sequence":"additional","affiliation":[{"name":"Department of Plastic, Reconstructive and Aesthetic Surgery, Campus Bio-Medico of Rome University, Rome"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7179-3533","authenticated-orcid":false,"given":"Giovanni Francesco","family":"Marangi","sequence":"additional","affiliation":[{"name":"Department of Plastic, Reconstructive and Aesthetic Surgery, Campus Bio-Medico of Rome University, Rome"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5266-6379","authenticated-orcid":false,"given":"Carolina","family":"Nobile","sequence":"additional","affiliation":[{"name":"Department of Hematology, Stem Cell Transplantation, Transfusion Medicine and Cellular Therapy, Campus Bio-Medico of Rome University, Rome"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5506-8323","authenticated-orcid":false,"given":"Mario","family":"Alessandri-Bonetti","sequence":"additional","affiliation":[{"name":"Department of Plastic, Reconstructive and Aesthetic Surgery, Campus Bio-Medico of Rome University, Rome"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0789-779X","authenticated-orcid":false,"given":"Chiara","family":"Gregorj","sequence":"additional","affiliation":[{"name":"Department of Hematology, Stem Cell Transplantation, Transfusion Medicine and Cellular Therapy, Campus Bio-Medico of Rome University, Rome"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1346-0204","authenticated-orcid":false,"given":"Vincenzo","family":"Cerbone","sequence":"additional","affiliation":[{"name":"Department of Orthopedics and Traumatology, II University Clinic, G. Pini Orthopaedic Institute, Milan, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7375-1524","authenticated-orcid":false,"given":"Marco","family":"Gratteri","sequence":"additional","affiliation":[{"name":"Department of Plastic, Reconstructive and Aesthetic Surgery, Campus Bio-Medico of Rome University, Rome"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3882-4171","authenticated-orcid":false,"given":"Erika","family":"Caldaria","sequence":"additional","affiliation":[{"name":"Department of Plastic, Reconstructive and Aesthetic Surgery, Campus Bio-Medico of Rome University, Rome"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4645-4465","authenticated-orcid":false,"given":"Maria Cristina","family":"Tirindelli","sequence":"additional","affiliation":[{"name":"Department of Hematology, Stem Cell Transplantation, Transfusion Medicine and Cellular Therapy, Campus Bio-Medico of Rome University, Rome"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8857-6060","authenticated-orcid":false,"given":"Paolo","family":"Persichetti","sequence":"additional","affiliation":[{"name":"Department of Plastic, Reconstructive and Aesthetic Surgery, Campus Bio-Medico of Rome University, Rome"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"194","published-online":{"date-parts":[[2022,3,22]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","first-page":"457","DOI":"10.21037\/atm.2016.12.44","article-title":"An update and review of cell-based wound dressings and their integration into clinical practice","volume":"4","author":"A Pourmoussa","year":"2016","journal-title":"Ann Transl Med"},{"key":"ref2","doi-asserted-by":"crossref","first-page":"1607","DOI":"10.1097\/PRS.0000000000001292","article-title":"Differential contributions of graft-derived and host-derived cells in 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