{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T11:57:06Z","timestamp":1773403026454,"version":"3.50.1"},"reference-count":0,"publisher":"Biomedical Informatics","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bioinformation"],"published-print":{"date-parts":[[2021,5,31]]},"abstract":"<jats:p>Zizyphus spina-christi (Rhamnaceae family) is an edible plant used in folk medicine. Therefore, it is of interest to report the cytotoxic effects of Z. spina-christi bark crude extract on human cell lines. Crude ethanol extract of Z. spina-christi bark was fractionated with increasing polarity (diethyl ether, chloroform, ethyl acetate and butanol fractions). The fractions were examined for their cytotoxicity against human colon cancer (HCT-116 and CACO-2), cervical cancer (HeLa and HEp-2), lung carcinoma (A-549), hepatocellular carcinoma (HepG-2), breast cancer (MCF-7) and prostate cancer (PC-3) cell lines using viability assay. Diethyl ether fraction of Z. spina-christi showed the highest cytotoxic effects among the four extracts of Z. spina-christi. The IC50 of diethyl ether fraction was 7.14, 11.2, 11.6, 15.4, 39.8, 42.2, 84.2 and 153.8 \u0134g\/ml on HepG-2, A-549, CACO-2, HCT-116, MCF-7, PC-3, HeLa, and HEp-2 cell lines, respectively. Data shows that the diethyl ether fraction of Z. spina-christi showed effective cytotoxic effects in colon, lung and hepatocellular cancer cell lines.<\/jats:p>","DOI":"10.6026\/97320630017583","type":"journal-article","created":{"date-parts":[[2021,12,9]],"date-time":"2021-12-09T07:08:08Z","timestamp":1639033688000},"page":"583-592","source":"Crossref","is-referenced-by-count":2,"title":["In vitro cytotoxicity analysis of Zizyphus spina-christi stem bark extract on human cancer cell lines"],"prefix":"10.6026","volume":"17","author":[{"name":"Department of Chemistry, College of Sciences, University of Ha!il, Ha!il 55476, Kingdom of Saudi Arabia","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saravanan","family":"Rajendrasozhan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"4070","published-online":{"date-parts":[[2021,5,31]]},"container-title":["Bioinformation"],"original-title":[],"deposited":{"date-parts":[[2021,12,9]],"date-time":"2021-12-09T07:08:16Z","timestamp":1639033696000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.bioinformation.net\/017\/97320630017583.htm"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5,31]]},"references-count":0,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2021,5,31]]},"published-print":{"date-parts":[[2021,5,31]]}},"URL":"https:\/\/doi.org\/10.6026\/97320630017583","relation":{},"ISSN":["0973-8894","0973-2063"],"issn-type":[{"value":"0973-8894","type":"print"},{"value":"0973-2063","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,5,31]]}}}