{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T04:55:24Z","timestamp":1783140924330,"version":"3.54.6"},"reference-count":0,"publisher":"Biomedical Informatics","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bioinformation"],"published-print":{"date-parts":[[2024,9,30]]},"abstract":"<jats:p>Oral submucous fibrosis (OSMF) is a condition that may be cancerous. The prognosis of OSMF is determined by a number of biomarkers, including 8-hydroxy 2\u2032 de-oxy-guanosine and 8-isoprostane. It is possible to assess the levels of 8-OHdG and 8-isoprostane in blood and saliva. Therefore, it is of interest to estimate salivary 8-OHdG and 8-isoprostane levels in order to diagnose oral submucous fibrosis. A sample size of 40 was divided into two groups with 20 samples in each, i.e., Group I - Healthy group (gutka consumers without any lesion) and Group II -Test (gutka consumers with OSMF). Samples of serum and saliva were taken from each group. Then samples were centrifuged for 15\u201320 minutes at 1000 RPM and 2-8\u00b0C. The resulting supernatant was pipetted out into labelled Eppendorf tubes in a volume of 1.5 ml, and it was then kept at 80\u00b0C. 8-OHdG and 8-isoprostane concentrations in various samples were determined using the ELISA technique. Serum's 8-OHdG content was considerably lower than saliva sample (P-value &lt;0.05). The test group exhibited increased concentrations of 8-OHdG and 8-isoprostane in both saliva and serum samples when compared to the control group. 8-OHdG and 8-isoprostane can be utilised to diagnoses of OSMF.<\/jats:p>","DOI":"10.6026\/9732063002001042","type":"journal-article","created":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T09:31:58Z","timestamp":1738402318000},"page":"1042-1045","source":"Crossref","is-referenced-by-count":5,"title":["Early diagnosis of oral submucous fibrosis using salivary 8-OHDG and 8-Isoprostane"],"prefix":"10.6026","volume":"20","author":[{"name":"Department of Oral Pathology and Microbiology, Sudha Rustagi College of Dental Sciences and Research, Faridabad, Haryana, India","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bireswar","family":"Roy","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaili","family":"Ghosh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Kalinga Institute of Dental Sciences, Bhubaneswar, Odisha, India","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"SS","family":"Mohamed Abdulcader Riyaz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Oral and Maxillofacial Diagnostic Sciences, College of Dentistry, Ar Rass Branch, Qassim University, Saudi Arabia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madhvika","family":"Patidar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Oral Pathology and Microbiology, Government College of Dentistry, Indore, MP, India","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Urmi","family":"Mehta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Dental Surgeon, Gujarat, India","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Priya","family":"Agarwal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Oral Medicine and Radiology, Modern Dental College and Research Centre, Indore, MP, India","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pushkar","family":"Gupta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Prosthodontics and Crown & Bridge, Hitkarini Dental College and Hospital, Jabalpur, MP, India","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4070","published-online":{"date-parts":[[2024,9,30]]},"container-title":["Bioinformation"],"original-title":[],"deposited":{"date-parts":[[2025,2,1]],"date-time":"2025-02-01T09:31:59Z","timestamp":1738402319000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.bioinformation.net\/020\/9732063002001042.htm"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,30]]},"references-count":0,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2024,9,30]]},"published-print":{"date-parts":[[2024,9,30]]}},"URL":"https:\/\/doi.org\/10.6026\/9732063002001042","relation":{},"ISSN":["0973-8894","0973-2063"],"issn-type":[{"value":"0973-8894","type":"print"},{"value":"0973-2063","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9,30]]}}}