{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T07:31:36Z","timestamp":1791617496045,"version":"4.3.4"},"reference-count":0,"publisher":"Biomedical Informatics","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bioinformation"],"published-print":{"date-parts":[[2024,11,30]]},"abstract":"<jats:p>This study investigates the application of Oil Red O and Sudan stains in identifying and quantifying lipid accumulation in adipose tissue, specifically focusing on its relevance to obesity-related oral diseases. Adipose tissue samples were collected from 50 obese patients (BMI &gt; 30) and 50 normal-weight controls. Samples were stained with Oil Red O and Sudan III, IV and Black. Lipid accumulation was quantified using digital image analysis. Oral health examinations assessed the prevalence of periodontal disease, dental caries, and oral candidiasis. Oil Red O staining show 2.8-fold higher lipid contents obese subjects than controls (p&lt;0.001). Sudan stains demonstrated similar trends, with Sudan Black being the most sensitive (3.2-fold increase, p&lt;0.001). Positive correlations were found between lipid accumulation and the severity of periodontal disease (r=0.72, p&lt;0.001), dental caries (r=0.58, p&lt;0.01), and oral candidiasis (r=0.63, p&lt;0.01) in obese subjects. Oil Red O and Sudan stains effectively identify and quantify lipid accumulation in adipose tissue. The study's findings underscore a robust link between increased lipid content and the prevalence of obesity-related oral diseases, highlighting the potential of these staining techniques in oral health research and clinical practice.<\/jats:p>","DOI":"10.6026\/9732063002001663","type":"journal-article","created":{"date-parts":[[2025,2,3]],"date-time":"2025-02-03T04:37:17Z","timestamp":1738557437000},"page":"1663-1666","source":"Crossref","is-referenced-by-count":4,"title":["Identification and quantification of lipid accumulation in adipose tissue using oil red O and Sudan stains"],"prefix":"10.6026","volume":"20","author":[{"name":"Department of Biochemistry, Government Medical College, Datia, Madhya Pradesh, India","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Swati","family":"Shrivastava","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aishwarya","family":"Srivastava","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Biochemistry, All India Institute of Medical Sciences, Gorakhpur, Uttar Pradesh, India","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruchi","family":"Jain","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Anatomy, MLB Medical College, Jhansi, Uttar Pradesh, India","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amit Kumar","family":"Srivastava","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Anatomy, Government Medical College Datia, Madhya Pradesh, India","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sourabh","family":"Shrivastava","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Anaesthesiology, GAJRA Raja Medical College, Gwalior, Madhya Pradesh, India","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rashmi","family":"Sathe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Oral Medicine and Radiology, People's College of Dental Sciences and Research Centre, Bhopal, Madhya Pradesh, India","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4070","published-online":{"date-parts":[[2024,11,30]]},"container-title":["Bioinformation"],"original-title":[],"deposited":{"date-parts":[[2025,2,3]],"date-time":"2025-02-03T04:37:22Z","timestamp":1738557442000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.bioinformation.net\/020\/9732063002001663.htm"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,30]]},"references-count":0,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2024,11,30]]},"published-print":{"date-parts":[[2024,11,30]]}},"URL":"https:\/\/doi.org\/10.6026\/9732063002001663","relation":{},"ISSN":["0973-8894","0973-2063"],"issn-type":[{"value":"0973-8894","type":"print"},{"value":"0973-2063","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11,30]]}}}