{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,13]],"date-time":"2026-11-13T01:12:06Z","timestamp":1794532326991,"version":"4.9.1"},"reference-count":0,"publisher":"Biomedical Informatics","issue":"07","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bioinformation"],"published-print":{"date-parts":[[2025,7,31]]},"abstract":"<jats:p>The effect of Nucleic Acid Testing (NAT) and Chemiluminescent Immunoassay (CLIA) in detecting TTIs (HIV, HBV, HCV, Syphilis and Malaria by rapid card) among 30,335 blood donations, with a focus on 1,843 reactive units is of interest. NAT showed superior sensitivity (98.50% for HBV, 98% for HIV and 97.50% for HCV) compared to CLIA (94.44.0% for HIV, 79.09% for HBV, 64.20% for HCV), but both methods exhibited high false-positive rates (37.7% for NAT, up to 70.6% for CLIA-HCV). NAT had specificity for HIV (98.5%), HBV (98%) and HCV (98%). CLIA exhibited high false positives (HBV: 27.1%, HCV: 16.5%, HIV: 5.7%), while NAT yield identified 106 HBV (0.35%) and 63 HCV (0.2%) additional cases. NAT was cost-effective for HBV and HCV but less so for HIV. Thus, NAT\u2019s role as a highly sensitive screening tool and with CLIA requiring confirmatory testing to optimize blood supply efficiency is shown.<\/jats:p>","DOI":"10.6026\/973206300211947","type":"journal-article","created":{"date-parts":[[2025,8,11]],"date-time":"2025-08-11T03:37:46Z","timestamp":1754883466000},"page":"1947-1951","source":"Crossref","is-referenced-by-count":2,"title":["Comparative analysis of NAT reactivity and CLIA in detecting transfusion transmitted Infection among blood donors"],"prefix":"10.6026","volume":"21","author":[{"name":"Department of Transfusion Medicine, Uttar Pradesh University of Medical Sciences, Saifai, Etawah, Uttar Pradesh-206130, India","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jyoti Kala","family":"Bharati","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arvind Kumar","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yatendra","family":"Mohan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaditya","family":"Shivhare","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shweta","family":"Chaudhary","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nouratan","family":"Singh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Transfusion Medicine, Uttar Pradesh University of Medical Sciences, Saifai, Etawah, Uttar Pradesh-206130, India","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4070","published-online":{"date-parts":[[2025,7,31]]},"container-title":["Bioinformation"],"original-title":[],"deposited":{"date-parts":[[2025,8,11]],"date-time":"2025-08-11T03:37:47Z","timestamp":1754883467000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.bioinformation.net\/021\/973206300211947.htm"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,31]]},"references-count":0,"journal-issue":{"issue":"07","published-online":{"date-parts":[[2025,7,31]]},"published-print":{"date-parts":[[2025,7,31]]}},"URL":"https:\/\/doi.org\/10.6026\/973206300211947","relation":{},"ISSN":["0973-8894","0973-2063"],"issn-type":[{"value":"0973-8894","type":"print"},{"value":"0973-2063","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,31]]}}}