{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T05:53:07Z","timestamp":1787032387124,"version":"build-2736575974"},"reference-count":0,"publisher":"Biomedical Informatics","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bioinformation"],"published-print":{"date-parts":[[2025,10,31]]},"abstract":"<jats:p>Oral submucous fibrosis (OSF), a potentially malignant disorder, is strongly linked to areca nut consumption, with arecoline inducing epithelial-mesenchymal transition (EMT) in fibroblasts. Therfeore, it is of interest to investigate EMT marker expression in OSF fibroblasts exposed to arecoline and assessed the inhibitory effects of curcumin and epigallocatechin-3-gallate (EGCG). Primary fibroblasts from OSF and normal mucosa were treated and EMT markers were analyzed by RT-qPCR, Western blotting and wound healing assays. Arecoline decreased E-cadherin and increased N-cadherin, vimentin, \u03b1-SMA, Snail, Twist and migration rate, whereas co-treatment with curcumin or EGCG significantly reversed these effects, with curcumin showing greater efficacy. Thus, we show that curcumin and EGCG inhibit arecoline-induced EMT and may serve as potential therapeutic agents in OSF management.<\/jats:p>","DOI":"10.6026\/973206300213871","type":"journal-article","created":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T06:52:38Z","timestamp":1762411958000},"page":"3871-3875","source":"Crossref","is-referenced-by-count":1,"title":["Gene expression analysis of epithelial-mesenchymal transition markers in oral submucous fibrosis fibroblasts treated with arecoline"],"prefix":"10.6026","volume":"21","author":[{"name":"Department of Dentistry, GMERS Medical College, Vadnagar, Gujarat, India","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jigar","family":"P Thakkar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gopal","family":"Srivastava","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Oral Medicine and Radiology, Government Dental College, Thiruvananthapuram, Kerala, India","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khushali","family":"Shah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Oral and maxillofacial Pathology, K.M. Shah Dental College and Hospital, Sumandeep Vidyapeeth Deemed to be university, Vadodara, Gujarat, India","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vipin","family":"Arora","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Restorative Dental Sciences, Faculty of Dentistry, Taif University, Taif, Kingdom of Saudi Arabia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mony","family":"Behera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Oral Medicine and Radiology, Kalinga Institute of Dental Sciences (KIDS), Kalinga Institute of Industrial Technology (KIIT) Deemed to be University, Bhubaneswar-751024, Odisha, India","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sameer","family":"Gupta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Oral & Maxillofacial Surgery, Inderprastha Dental College and Hospital, Sahibabad, Ghaziabad, Uttar Pradesh, India","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4070","published-online":{"date-parts":[[2025,10,31]]},"container-title":["Bioinformation"],"original-title":[],"deposited":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T06:52:39Z","timestamp":1762411959000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.bioinformation.net\/021\/973206300213871.htm"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,31]]},"references-count":0,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2025,10,31]]},"published-print":{"date-parts":[[2025,10,31]]}},"URL":"https:\/\/doi.org\/10.6026\/973206300213871","relation":{},"ISSN":["0973-8894","0973-2063"],"issn-type":[{"value":"0973-8894","type":"print"},{"value":"0973-2063","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,31]]}}}