{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,4]],"date-time":"2026-04-04T11:48:56Z","timestamp":1775303336095,"version":"3.50.1"},"reference-count":0,"publisher":"Biomedical Informatics","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Bioinformation"],"published-print":{"date-parts":[[2026,3,31]]},"abstract":"<jats:p>Klebsiella pneumoniae is a major opportunistic pathogen and an important cause of multidrug-resistant gram-negative infections, driven largely by the spread of ESBL and carbapenemase enzymes. Therefore, it is of interest to analyse the phenotypic resistance patterns and molecular resistome of 100 non-duplicate clinical isolates collected from a tertiary care hospital in North India using CLSI-guided susceptibility testing, mCIM\/eCIM assays and PCR detection of resistance genes. Overall, 51% of isolates were MDR, with high resistance to cephalosporins, fluoroquinolones and penicillins and the highest carbapenem resistance was observed for ertapenem (57%). Carbapenem-resistant isolates comprised 40%, of which 70% produced metallo-\u03b2-lactamases and 10% serine \u03b2-lactamases, while ESBL genes (blaCTX-M-1 54%, blaSHV 50%, blaTEM 28%) and carbapenemases (blaNDM 22%, blaOXA-48 23%) were frequently detected, including ESBL\u2013carbapenemase co-harbouring in 35% and a \u2015super-resistome\u2016 in 2%. Thus, we show the urgent need for sustained molecular surveillance, strict infection control and strengthened antimicrobial stewardship to curb the dissemination of highly resistant K. pneumoniae<\/jats:p>","DOI":"10.6026\/973206300221295","type":"journal-article","created":{"date-parts":[[2026,4,4]],"date-time":"2026-04-04T10:23:36Z","timestamp":1775298216000},"page":"1295-1301","source":"Crossref","is-referenced-by-count":0,"title":["Molecular resistome profiling of multidrug-resistant klebsiella pneumoniae from a tertiary-care hospital in North India"],"prefix":"10.6026","volume":"22","author":[{"given":"Rashmi","family":"Rashmi","sequence":"first","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine, King George\u2019s Medical University, Lucknow, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheetal","family":"Verma","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine, King George\u2019s Medical University, Lucknow, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vimala","family":"Venkatesh","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine, King George\u2019s Medical University, Lucknow, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Upma","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine, King George\u2019s Medical University, Lucknow, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"4070","published-online":{"date-parts":[[2026,3,31]]},"container-title":["Bioinformation"],"original-title":[],"deposited":{"date-parts":[[2026,4,4]],"date-time":"2026-04-04T10:50:52Z","timestamp":1775299852000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.bioinformation.net\/022\/973206300221295.htm"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,31]]},"references-count":0,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2026,3,31]]},"published-print":{"date-parts":[[2026,3,31]]}},"URL":"https:\/\/doi.org\/10.6026\/973206300221295","relation":{},"ISSN":["0973-8894","0973-2063"],"issn-type":[{"value":"0973-8894","type":"print"},{"value":"0973-2063","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,31]]}}}